Search
Invitrogen
Cyclic AMP Competitive ELISA Kit
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
产品规格
检测灵敏度
0.037 pmol/mL
测定范围
0.078-20 pmol/mL
检测样本类型/体积
{Cell Lysate=100 µL, Tissue Homogenate=100 µL}
手工操作时间
1 hr 20 min
总时间
3 hr
均相（无需洗涤）
No
批间差异
7.1%
批内差异
6.5%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96-well plate
Detection Antibody
AP Conjugate
Neutralizing Reagent
Triethylamine
Acetic Anhydride
Standard
20X Wash Buffer
pNpp Substrate Solution
Stop Solution
Plate Sealer
Detection Antibody
AP Conjugate
Neutralizing Reagent
Triethylamine
Acetic Anhydride
Standard
20X Wash Buffer
pNpp Substrate Solution
Stop Solution
Plate Sealer
运输条件
Dry ice
储存
-20°C
蛋白名称
cAMP
Target site-specificity
蛋白家族
Other Proteins
检测试剂盒格式
Competitive ELISA
标签或染料
AP
关于本试剂盒
The cyclic AMP Competitive ELISA quantitates cAMP in cell or tissue lysates. The assay will exclusively recognize both natural and recombinant cyclic AMP.
原理方法
The cyclic AMP solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, binding to the target on a different epitope from the capture antibody. A conjugated enzyme has been incorporated into the assay. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is inversely proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
Cyclic adenosine monophosphate (cAMP) plays a key role as an intracellular second messenger for transduction events that follow a number of extracellular signals. The G-Protein Coupled Receptors (GPCR) is the largest family of cell surface receptors. They can be activated by different ligands, such as neurotransmitters, hormones, ions, small molecules, peptides, and other physiological signaling molecules. Typically, the binding of the ligands to its receptor resulting in the activation of G-proteins, in return, activates the effector adenylyl cyclase evoking the production of cAMP. The activation of a protein kinase by cAMP results in the phosphorylation of substrate proteins. Currently successful drugs in marketing have been developed to target these receptors. Among the GPCRs, ~367 receptors are potential drug development targets, but only about 20 have been used to generate therapeutically and commercially successful drugs so far. Because the involvement of cAMP can amplify the response of the ligand binding, the second messenger cAMP has been largely employed to monitor the activation of the GPCR to facilitate the therapeutic drug discovery.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名 : Cyclic AMP
