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Invitrogen
Cyclic GMP Competitive ELISA Kit
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产品规格
检测灵敏度
0.059 pmol/mL
测定范围
0.8-5000 pmol/mL
检测样本类型/体积
{Cell Lysate=100 µL, Supernatant=100 µL, Tissue Homogenate=100 µL}
手工操作时间
1 hr 20 min
总时间
3 hr
均相（无需洗涤）
No
批间差异
7.5%
批内差异
5.5%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96-well plate
Detection Antibody
AP Conjugate
Neutralizing Reagent
Triethylamine
Acetic Anhydride
Standard
20X Wash Buffer
pNpp Substrate Solution
Stop Solution
Plate Sealer
Detection Antibody
AP Conjugate
Neutralizing Reagent
Triethylamine
Acetic Anhydride
Standard
20X Wash Buffer
pNpp Substrate Solution
Stop Solution
Plate Sealer
运输条件
Dry ice
储存
-20°C
蛋白名称
cGMP
Target site-specificity
蛋白家族
Other Proteins
检测试剂盒格式
Competitive ELISA
标签或染料
AP
关于本试剂盒
The cyclic GMP Competitive ELISA quantitates cGMP in cell or tissue lysates. The assay will exclusively recognize both natural and recombinant cyclic GMP.
原理方法
The cyclic GMP solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is inversely proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
Guanosine cyclic 3',5'-(hydrogen phosphate) is a guanine nucleotide containing one phosphate group which is esterified to the sugar moiety in both the 3'- and 5'-positions. Cyclic gmp is a cellular regulatory agent and has been described as a second messenger. Its levels increase in response to a variety of hormones, including acetylcholine, insulin, and oxytocin and it has been found to activate specific protein kinases. [National Center for Biotechnology Information. PubChem Compound Database; CID=24316].
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名 : Cyclic GMP, Cyclic guanosine monophosphate, C10H12N5O7P, 3', 5'-cyclic GMP
