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Invitrogen
Human C1q ELISA Kit
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产品规格
检测灵敏度
0.08 ng/mL
测定范围
0.9-60 ng/mL
检测样本类型/体积
{Plasma=10 µL, Serum=10 µL, Supernatant=10 µL}
手工操作时间
1 hr 20 min
总时间
4 hr 30 min
均相（无需洗涤）
No
批间差异
4.2%
批内差异
4.7%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Lysis Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Lysis Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
2-8°C
蛋白名称
C1q
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Human C1q ELISA quantitates Hu C1q in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu C1q.
原理方法
The Human C1q solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
C1q is a sub-component of complement C1. C1q associates with complement components C1r and C1s, which are required for the progression of the proteolytic complement cascade. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. The A-, B-, and C-chains are arranged in the order A-C-B on chromosome 1.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
基因别名 : C1Q-C, C1QA, C1QB, C1QC, C1QG
基因ID : (Human) 712, (Human) 713, (Human) 714
基因符号 : C1QA, C1QB, C1QC
蛋白别名 : complement C1q chain A, complement C1q chain B, Complement C1q subcomponent subunit A, Complement C1q subcomponent subunit B, Complement C1q subcomponent subunit C, complement component 1, q subcomponent, A chain, alpha polypeptide, B chain, beta polypeptide, C chain, gamma polypeptide, complement component C1q, complement subcomponent C1q chain B
UniProt ID (Human) P02745, (Human) P02746, (Human) P02747
