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Invitrogen
Human CD40L (Soluble) Instant ELISA™ Kit
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产品规格
检测灵敏度
0.062 ng/mL
测定范围
0.16-10.0 ng/mL
检测样本类型/体积
{Plasma=15 µL, Serum=15 µL, Supernatant=15 µL}
手工操作时间
15 min
总时间
2 hr 10 min
均相（无需洗涤）
No
批间差异
14.2%
批内差异
6.8%
检测仪器
Absorbance Microplate Reader
产品规格
128 Tests
产品成分
Pre-coated 96 well Microplate
Detection Antibody
Anti-IgG-HRP
Sample Diluent, lyophilized
Standard (8-well strips x 4)
Wash Buffer Concentrate
Substrate Solution
Sample Diluent
Stop Solution
Lysis Buffer 10x
Adhesive Plate Covers
Detection Antibody
Anti-IgG-HRP
Sample Diluent, lyophilized
Standard (8-well strips x 4)
Wash Buffer Concentrate
Substrate Solution
Sample Diluent
Stop Solution
Lysis Buffer 10x
Adhesive Plate Covers
运输条件
Wet ice
储存
-20°C
蛋白名称
CD154 (CD40 Ligand)
Target sub-specificity
Soluble
均相（无需洗涤）
Human
检测试剂盒格式
Instant ELISA Kit
检测抗体结合物
HRP
标签或染料
HRP
关于本试剂盒
The Human Soluble CD40 Ligand(Hu sCD40L ELISA quantitates Hu sCD40L in human serum, plasma, cell culture supernatants, or other body fluids. The assay will exclusively recognize both natural and recombinant Hu sICD40L.
原理方法
The Human sCD40L solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
基因别名 : CD154, CD40L, CD40LG, gp39, hCD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP
基因ID : (Human) 959
基因符号 : CD40LG
蛋白别名 : CD154, CD40 antigen ligand, CD40 ligand, CD40-L, H-CD-40-L, M-CD-40-L, RP23-153G22.3, T-B cell-activating molecule, T-cell antigen Gp39, TNF-related activation protein, TRAP, tumor necrosis factor (ligand) superfamily member 5, Tumor necrosis factor ligand superfamily member 5
UniProt ID (Human) P29965
