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Human Cu/ZnSOD Matched Antibody Pair
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产品规格
检测灵敏度
0.08 ng/mL
测定范围
0.08-5 ng/mL
检测样本类型/体积
{Plasma=10 µL, Serum=10 µL, Supernatant=10 µL}
手工操作时间
1 hr 10 min
总时间
24 hr
均相（无需洗涤）
No
检测仪器
Absorbance Microplate Reader
产品规格
10 x 96 Tests
产品成分
Capture (coating) Antibody
Detection Antibody
Standard
Detection Antibody
Standard
运输条件
Wet ice
储存
-20°C
蛋白名称
SOD1
均相（无需洗涤）
Human
检测试剂盒格式
Matched Antibody Pair
检测抗体结合物
HRP
标签或染料
HRP
关于本试剂盒
The Human Cu/ZnSOD Matched Antibody Pair Kit comes with pre-matched antibody pairs and standards to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human Cu/ZnSOD. Buffer reagents and microplates needed to complete the ELISA reaction are sold separately.
原理方法
ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
靶标信息
SOD1 (superoxide dismutase) binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. This isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : ALS, ALS1, HEL-S-44, homodimer, hSod1, IPOA, SOD, SOD1
基因ID : (Human) 6647
基因符号 : SOD1
蛋白别名 : Cu/Zn superoxide dismutase, epididymis secretory protein Li 44, hSod1, indophenoloxidase A, SOD, soluble, Superoxide dismutase 1, Amyotrophic lateral sclerosis 1 (ALS1), Cu/Zn SOD, HGNC:11179, Indophenoloxidase A (IPOA), superoxide dismutase, Superoxide Dismutase 1 (SOD1), Superoxide dismutase [Cu-Zn], cystolic
UniProt ID (Human) P00441
