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Invitrogen
Human IL-23 ELISA Kit
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产品规格
检测灵敏度
4.0 pg/mL
测定范围
15.6-2,000 pg/mL
检测样本类型/体积
{Plasma=50 µL, Serum=50 µL, Supernatant=50 µL}
手工操作时间
1 hr 20 min
总时间
3 hr 45 min
均相（无需洗涤）
No
批间差异
6.3%
批内差异
5.9%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Sample Diluent
Assay Buffer concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
2-8°C
蛋白名称
IL-23
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Human Interleukin-23 (Hu IL-23) ELISA quantitates Hu IL-23 in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-23.
原理方法
The Human IL-23 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
IL-23 is a heterodimeric cytokine composed of the p40 subunit of IL-12 that is disulfide-linked with to two subunits, a p19 subunit unique to IL-23, and a p40 subunit that is shared with IL-12. IL-23 interacts with IL-12Rbeta1 and a beta2-like receptor subunit with STAT4 binding domain, IL-23R. IL-23 is secreted by activated mouse and human dendritic cells and is involved in the inflammation process and associated with autoimmune diseases. Mouse IL-23 was found not to induce significant amounts of IFN-gamma, induces strong proliferation of memory T cells (but not naive T cells), and activate mouse memory T cells to produce the proinflammatory cytokine IL-17. On the other hand, human IL-23 induces proliferation of memory T cells, induces moderate levels of IFN-gamma, and is produced by naive and memory T cells. IL-23 plays an important role in stimulating memory T-cells, and is required for the induction, expansion, maintenance, and downstream effector functions of Th17 cells, which play a vital role in upregulating neutrophil chemokines and various pro-inflammatory cytokines. IL-23 interacts with the receptors IL-12R beta1 and IL-23R to activate the Jak-Stat signaling cascade. IL-23 has been shown to possess potent anti-tumor and anti-metastatic activity in mouse models of cancer, suggesting a potential role for IL-23 in therapeutic treatment of cancer.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
基因别名 : IL-23, IL-23A, IL23A, IL23P19, P19, SGRF, UNQ2498/PRO5798
基因ID : (Human) 51561
基因符号 : IL23A
蛋白别名 : IL-23 subunit alpha, IL-23-A, Interleukin-23 subunit p19, IL-23p19, IL23, ILN, Interleukin, interleukin 23 p19 subunit, interleukin 23, alpha subunit p19, Interleukin-23 subunit alpha, interleukin-six, G-CSF related factor, JKA3 induced upon T-cell activation
UniProt ID (Human) Q9NPF7
