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Invitrogen
Human IL1RA Matched Antibody Pair
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产品规格
检测灵敏度
<31.2 pg/mL
测定范围
31.2-2,000 pg/mL
检测样本类型/体积
{Plasma=100 µL, Serum=100 µL, Supernatant=100 µL}
手工操作时间
1 hr 30 min
总时间
24 hr
均相（无需洗涤）
No
检测仪器
Absorbance Microplate Reader
产品规格
10 x 96 Tests
产品成分
Capture (coating) Antibody
Detection Antibody
Standard
Streptavidin-HRP
Detection Antibody
Standard
Streptavidin-HRP
运输条件
Wet ice
储存
2-8°C
蛋白名称
IL1RA
均相（无需洗涤）
Human
检测试剂盒格式
Matched Antibody Pair
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Human IL-1RA; Antibody Pair Kit comes with pre-matched antibody pairs, standards, and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-1RA. Buffer reagents needed to complete the ELISA reaction are sold separate as a Buffer Kit for Antibody Pairs (Catalog No. CNB0011). The Assay Buffer included in this Buffer Kit can be used as a blocking reagent for ELISA plates as well as a diluent for ELISA standards and samples, detection antibody, and HRP conjugate.
原理方法
ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
靶标信息
The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein is a receptor for interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA). It is an important mediator involved in many cytokine induced immune and inflammatory responses. This gene along with interleukin 1 receptor, type II (IL1R2), interleukin 1 receptor-like 2 (IL1RL2), and interleukin 1 receptor-like 1 (IL1RL1) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
基因别名 : DIRA, ICIL-1RA, IL-1ra, IL-1ra3, IL-1RN, IL1F3, IL1RA, IL1RN, IRAP, MVCD4
基因ID : (Human) 3557
基因符号 : IL1RN
蛋白别名 : ICIL-1RA, IL-1RN, IL-1ra, IRAP, IL1 inhibitor, CD121A, D2S1473, IL 1 receptor antagonist, il1 receptor, il-1 receptor, interleukin-1 receptor, MGC10430, P80, IL1 receptor antagonist, IL1R, il-1r, IL1-RA, IL-1R-alpha, IL1RN (IL1F3), Interleukin-1 receptor antagonist protein, intracellular IL-1 receptor antagonist type II, intracellular interleukin-1 receptor antagonist (icIL-1ra), type II interleukin-1 receptor antagonist
UniProt ID (Human) P18510
