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Invitrogen
Human SARS-CoV-2 Spike (Trimer) Ig Total ELISA Kit
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产品规格
检测灵敏度
<62.5 U/mL
测定范围
62.5-4,000 U/mL
检测样本类型/体积
{Serum=5 µL, Plasma=5 µL}
手工操作时间
30 min
总时间
1 hr 15 min
均相（无需洗涤）
No
批间差异
5%
批内差异
4.3%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
High Control (standard), Calibrator (medium control), Low Control
Anti-Ig Total - HRP conjugate
Assay Buffer concentrate
Wash Buffer concentrate
Substrate solution
Stop Solution
Adhesive Plate Covers
High Control (standard), Calibrator (medium control), Low Control
Anti-Ig Total - HRP conjugate
Assay Buffer concentrate
Wash Buffer concentrate
Substrate solution
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
2-8°C
蛋白名称
SARS-CoV-2 Spike (trimer) Protein
均相（无需洗涤）
Human
检测试剂盒格式
Direct ELISA
检测抗体结合物
HRP
标签或染料
HRP
关于本试剂盒
The Human SARS-CoV-2 Spike (Trimer) Ig Total ELISA is a serology assay that measures and quantitates immunoglobulin (Ig) antibodies against SARS-CoV-2 Spike (Trimer) in human serum or plasma.
原理方法
The Human SARS-CoV-2 Spike (Trimer) Ig Total ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of Ig antibodies bound to SARS-CoV-2 Spike (Trimer). A trimerized Spike protein is pre-coated in the wells of the supplied microplate. Samples, controls, including a high control that can be used as a standard are then added into these wells and bind to the immobilized (capture) Spike protein. The wells are washed and Ig antibodies conjugated to HRP are added and will bind to any captured antibodies. The Ig Total antibody recognizes IgG, IgM and IgA. The wells are washed and a substrate solution is added that reacts with the enzyme complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of antibody present in the original specimen.
靶标信息
It has recently been shown that SARS (severe acute respiratory syndrome) is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness, such as the common cold, in humans. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The glycosylated spike protein (as well as the nucleocapsid protein) can be detected in infected cell culture supernatants with antisera from SARS patients.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名 : Coronavirus, COVID, Covid 19, COVID19, COVID-19, SARS, SARS Coronavirus, SARS CoV, SARS CoV2, SARS CoV-2, SARS Spike Protein, SARS-CoV-2
