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Invitrogen
Human TACE/ADAM17 ELISA Kit
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产品规格
检测灵敏度
70 pg/mL
测定范围
70-5,000 pg/mL
检测样本类型/体积
{Plasma=50 µL, Serum=50 µL, Supernatant=100 µL}
手工操作时间
1 hr 20 min
总时间
4 hr 45 min
均相（无需洗涤）
No
批间差异
<12%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
Standard
Assay Diluent concentrate
Biotinylated Detection Antibody
SAV-HRP
Wash Buffer
Chromogen
Stop Solution
Adhesive Plate Covers
运输条件
Wet ice
储存
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
蛋白名称
ADAM17
蛋白家族
Proteases Peptidases & Inhibitors
均相（无需洗涤）
Human
检测试剂盒格式
Sandwich ELISA Kit
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The Human TACE/ADAM17 ELISA quantitates Hu TACE in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu TACE.
原理方法
The Human TACE solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
严格验证
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
ADAM17 (disintegrin and metalloproteinase domain-containing protein 17) cleaves the membrane bound precusor of TNF-alpha to its mature soluble form. ADAM 17 is responsbile for the proteolytical release of soluble JAM3 from endothelial cells surface and the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1, and the amyloid precursor protein. It acts as an activator of the Notch pathway by mediating cleavage of Notch and generating the membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). Mutations in the gene can result in inflammatory skin and bowel disease, neonatal 1.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
基因别名 : ADAM17, ADAM18, CD156B, CSVP, NISBD, NISBD1, TACE
基因ID : (Human) 6868
基因符号 : ADAM17
蛋白别名 : ADAM 17, Snake venom-like protease, TNF-alpha convertase, TNF-alpha-converting enzyme, a disintegrin and metalloproteinase, ADAM, ADAMs, CD156b antigen, metalloendopeptidases, ADAM metallopeptidase domain 18, CD156b, Disintegrin and metalloproteinase domain-containing protein 17, TNF-alpha converting enzyme, tumor necrosis factor, alpha, converting enzyme
UniProt ID (Human) P78536
