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Invitrogen
PTGES2 Competitive ELISA Kit
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产品规格
检测灵敏度
18.75 pg/mL
测定范围
31.25-2,000 pg/mL
检测样本类型/体积
{Plasma=50 µL, Serum=50 µL}
手工操作时间
1 hr 20 min
总时间
2 hr 30 min
均相（无需洗涤）
No
批间差异
<10%
批内差异
<10%
检测仪器
Absorbance Microplate Reader
产品规格
96 Tests
产品成分
Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
运输条件
Wet ice
储存
Refer to product documentation for component specific storage temperature.
蛋白名称
PTGES2
蛋白家族
Prostaglandin
均相（无需洗涤）
All
检测试剂盒格式
Competitive ELISA
检测抗体结合物
Biotin
标签或染料
HRP
关于本试剂盒
The multispecies Prostaglandin E2 (PGE2) ELISA quantitates PGE2 in serum, plasma, and other biological fluids
原理方法:
The Competitive multispecies ELISA research-use-only kit is designed to quantitatively measure Prostaglandin E2 (PGE2) independent of species. A PGE2 standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to PGE2 is added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of PGE2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
靶标信息
The PTGES2 gene encodes for the enzyme prostaglandin E synthase 2 (PTGES2), which is crucial for the biosynthesis of prostaglandin E2 (PGE2) from arachidonic acid. This enzyme operates downstream of the cyclooxygenase (COX) pathway, particularly interacting with COX-1 and COX-2 enzymes. PTGES2 is involved in various physiological and pathological processes by generating PGE2, which functions in inflammation, fever generation, and pain. Notably, PTGES2 is a membrane-associated protein and is distinct in its role compared to other prostaglandin synthases due to its specific functional and regulatory properties. Its activity is essential in fine-tuning the levels of PGE2 in tissues, impacting numerous signaling cascades, including those involved in immune responses and vascular functions.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名 : FLJ14038, MGC11289, RP11-395P17.2
