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One Lambda™ LinkSēq™ technology combines sequence-specific primers and fluorescent SYBR Green DNA-binding dye to generate low-resolution typing results in as little as 90 minutes with DNA extracted from whole blood. LinkSēq kits are simple and efficient for HLA Class I and Class II genotyping, without the need for gel electrophoresis.
LinkSeq kits are available in single sample or multi-sample formats on a number of qPCR platforms for easy integration into any lab.
Data generated with LinkSēq kits are analyzed with SureTyper™ software. This bidirectional analysis software manages the entire data typing process and includes full reporting, auditing, and security features.
Workflow
LinkSeq Workflow is a fast, flexible, easy and effective typing solution with results in as little as 90 minutes.
LinkSēq HLA Typing Kits
LinkSēq HLA Typing Kits provide the most complete solution for laboratories interested in fast and easy typing of all 11 loci. This product provides low resolution typing for HLA A, B, C, DRB1, DRB345, DQA1, DQB1, DPA1, and DPB1, as well as common null alleles.
Features
- Molecular detection using SYBR Green Real-Time PCR
- Genotyping of Class I and II loci
- Single antigen bead coverage for all 11 loci
- Kits for targeting genotyping of specific loci or genetic specific markers
- Automated analysis with SureTyper for HLA
LinkSēq KIR Typing Kits
LinkSēq KIR typing kits are a fast and effective real-time PCR genotyping solution for KIR alleles.
Killer cell immunoglobulin-like receptors (KIR) are a family of cell surface receptors found on human Natural Killer (NK) cells. Certain KIR types interact with HLA Class I molecules to inhibit the activity of NK cells while other KIR types can stimulate NK activity.
Features
- Molecular detection using SYBR Green Real-Time PCR
- Genotyping of all 15 KIR genes
- Variable throughput with single sample or multi-sample kits
- Automated analysis with SureTyper for HLA in RUO mode
LinkSēq ABO genotyping and subgrouping
ABO alleles encode the enzyme Glycosyltransferase. Many A subgroups involve a weakly expressed A antigen, which can cause discordant serological reactions in forward and reverse typing. Our ABO products offer a better solution to research these alleles by separating A1 from non-A1, B from B3, Bweak and Bel, and much more.
Coverage includes
- A, B, AB, cisAB, O
- A subgroups: A1 or non-A1, i.e., A2, A3, Aweak, Ael or Am
- B subgroups: B, B3, Bweak or Bel
- O subgroups: O1, O2
- Possible Bombay phenotype
LinkSēq HPA Typing Kits
LinkSēq™ HPA typing kits are a fast and effective real-time PCR genotyping solution for evaluation of Human Platelet Antigens (HPA) glycoproteins expressed on platelet membranes. Research on HPA compatibility may help further aid in the understanding of transplant related immune complications.
Features
- Molecular detection using SYBR Green Real-Time PCR
- Genotyping of HPA-1 through HPA-11 and HPA-15
- Designed to be analyzed using SureTyper Blood software (RUO) to automatically call genotypes and predicts phenotypes
LinkSēq ancillary
Our accessories support the use of the core LinkSēq products and a key ancillary product is the LinkSēq Wipe Test. According to widely accepted Standards and Guidelines (e.g. ASHI and EFI), laboratories must routinely monitor for contamination of pre-amplification areas by the most common amplification products that are produced in the laboratory.
The LinkSēq Wipe Test was developed to detect both genomic DNA contamination as well as potential LinkSēq amplicon contamination. Additional products include accessories to ensure that your needs are covered.
Not all products are CE-marked in the EU or 510(k)-cleared in the US for in vitro diagnostic use. Availability of products in each country depends on local regulatory marketing authorization status.