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TEM tomography software for everyday 3D data acquisition
Thermo Scientific Tomography 5 Software supports tomographic data acquisition across diverse life sciences and materials science samples, including resin-embedded and cryogenically prepared samples. From investigating cellular structures and macromolecular complexes to characterizing nanoscale structures in 3D with full chemical and structural context, it offers the versatility and ease of use needed for advanced research.
Tomography 5 Software is compatible with the Thermo Scientific Talos 12 TEM, Thermo Scientific Spectra TEM, and other transmission electron microscopes without an Autoloader, offering flexible integration across your existing instruments.
Tomography 5 Software is also part of the Thermo Scientific CryoTomo Software Suite, which adds advanced automation capabilities and tilt series analysis to support higher-throughput cryo-electron tomography on Thermo Scientific Glacios or Thermo Scientific Krios Cryo-TEMs.
Tomography 5 Software benefits
Features of Tomography 5 Software for TEM 3D data acquisition
The Atlas is a low-magnification map of each grid, which makes it easy to assess grid quality, screen grids, and select sites for high-magnification imaging.
Search Maps are sets of tiles with medium magnification that allow you to map entire grid squares. They help increase data collection throughput and improve navigation accuracy.
Multisite batch tomography for automated data acquisition increases TEM productivity. Just navigate to several regions of interest and set up the tilt series. The fully automated data acquisition runs as soon as all settings are defined.
Off-axis multi-shot acquisition significantly enhances throughput. It records several tomogram positions with only one focus and tracking area, streamlining data acquisition, and increasing efficiency.
The dose symmetric acquisition scheme optimally distributes the electron dose for high-resolution sub-tomogram averaging. During a tomographic tilt series, the acquisition scheme exposes the projections in the lower negative and positive angular tilt range uniformly, preserving high-resolution information. This collection scheme optimizes resolution (i.e., the sub-nanometer range) and provides considerably better results than continuous or bi-directional tilt schemes.
Multiple cameras and detectors enable a wide range of use cases and increase versatility. Tomography 5 Software is fully integrated with Thermo Scientific Ceta CMOS Cameras, Thermo Scientific Falcon 4i Direct Electron Detectors, Panther STEM Detectors, and Ultra-X EDX Detectors.
The Thermo Scientific Selectris X Imaging Filter greatly improves the signal-to-noise ratio in tomography, boosting contrast and resolution through zero-loss filtering (i.e. the removal of inelastically scattered electrons). The imaging filter is fully integrated with Tomography 5 Software.
Tomography 5 Software resources
CryoFlow Software updates
For Research Use Only. Not for use in diagnostic procedures.