Before an antimicrobial susceptibility test guides a clinician's therapy or a culture medium reveals a clinically significant pathogen, each method must first demonstrate that it performs consistently, accurately, and reliably within the unique environment of the laboratory. Validation and verification are therefore far more than regulatory requirements — they are fundamental quality processes that safeguard patient outcomes, ensure compliance with international standards, and reinforce confidence in every diagnostic decision.
For Quality Control professionals and Technical Assistants, understanding these principles is essential to maintaining laboratory excellence. This guide provides a practical, science-based framework for planning, executing, documenting, and troubleshooting the verification of antimicrobial susceptibility testing (AST) systems and culture media, equipping laboratory teams with the knowledge needed to implement robust, compliant, and reproducible quality practices.
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