GeneMapper PG Software

Mixtures meet their match with a simpler, smarter, all-in-one mixture interpretation solution

Advancements in DNA extraction, STR chemistry, and capillary electrophoresis have dramatically increased the sensitivity of forensic testing, enabling the recovery of usable DNA from more samples than ever before. With increased sensitivity and the high demand for DNA evidence, complex mixture interpretation continues to pose one of the greatest challenges in forensic DNA analysis.

GeneMapper PG Software extends the trusted GeneMapper ID-X Software with a seamless, intuitive suite of software designed to bring clarity, deeper insight, and confidence to your mixture analysis. With GeneMapper PG Software, transparency isn’t just a feature—it’s the foundation. With visualizations and clear explanations, you can track the logic, compare models, and be confident in your results.

Step-by-step logic and visualization tools provide rationale for each decision, streamlining validation and strengthening expertise
Multiple models for data-driven number of contributor (NoC) estimations
A fully continuous probabilistic model for precise, reproducible mixture deconvolution
Likelihood ratio calculation tools for scenarios ranging from simple to complex
Robustness analysis test simulated profiles to determine the strength of the person of interest (POI) likelihood ratio

Video: Explore GeneMapper PG Software and see how transparent, deterministic probabilistic genotyping enhances DNA mixture interpretation, streamlines implementation, and ensures court-ready confidence

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Profile review

Start your interpretation with a big-picture view of your entire project. In the Profiles screen, manage your data for single or multiple cases within one project. Here you will find an evidence and reference list, allele table, built-in electropherogram views, and sample quality information. Within this screen, you can evaluate sample quality using built-in flags such as degradation and low peak height, group samples via custom tags, combine replicate amplifications into a single sample for analysis, and set initial (analyst evaluated) and final NoC results (post-Number of Contributors analysis) for each sample.

Number of contributors

Estimating the NoC is foundational to interpretation, informing every subsequent step. GeneMapper PG Software offers a variety of algorithms to determine the number of contributors in a DNA mixture. These algorithms range from basic methods like maximum allele count (MAC) to machine learning-based decision trees. Each method provides complete transparency, allowing you to scrutinize the estimation process in detail.

Deconvolution

Modeling biological aspects of forensic DNA profiles with customizable settings for locus, laboratory, STR kit, and instrument-specific values, the mixture deconvolution step employs the biological context optimized sampling (BCOS) algorithm. This deterministic algorithm follows a framework and fixed set of rules, for efficient and consistent analysis.

The software's powerful visualization tools and real-time explanations enable detailed analysis and exploration. Instantly view electropherograms to compare observed DNA profiles with expected solutions, extract major contributors, and export CODIS-compatible files for database searches.

Compare profiles

Whether examining pattern cases or tracking QC trends, profile comparison tools streamline data analysis across projects and cases. Heat maps highlight similarities and discrepancies across profiles, while a Match Table presents a list of best matches filtered by percentage to quickly identify the most relevant profiles.

Likelihood ratio calculations

Calculate even the most complex likelihood ratio (LR) scenarios. Build the scenario, visualize the result, and drill down into the calculation details. The powerful LR Robustness tool simulates thousands of profiles, configurable to be either unrelated or a specific family relationship to the POI, to produce an empirical cumulative distribution plot of likelihood ratios. By positioning your POI’s LR against these simulations, determine whether your LR result is significant or is possibly the result of a fortuitous match.

Human Identification Professional Services

HID professional services enable agencies to overcome critical implementation challenges through a collaborative partnership with Thermo Fisher, a worldwide leader in validated human identification solutions.

Professional services are available to support:

New laboratory development, implementation, and validation
Instrumentation and software integration
New protocol validation and testing
Workflow, software, and DNA theory training and educational services

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Validation

Thermo Fisher Scientific validated GeneMapper PG Software v1.0 following the FBI Quality Assurance Standards (July 1, 2025) and guidelines from the Scientific Working Group on DNA Analysis Methods (July 1, 2025). Each laboratory using GeneMapper PG Software v1.0 should perform its own internal verification or validation studies to establish interpretation criteria and demonstrate that the software is appropriate and fit for its own human identification uses.

Resources

Technical notes—developmental validation and model creation

Explore how GeneMapper PG Software delivers transparent, reproducible results for complex DNA mixtures. The Developmental Validation Report confirms performance under SWGDAM guidelines, demonstrating sensitivity, specificity, precision, and accuracy across one- to four-person mixtures on 3500 and SeqStudio Flex instruments.

The Creating a New Model note guides users through configuring a new STR kit models within the software—ensuring reliable likelihood ratio (LR) calculations and seamless integration with GeneMapper ID-X.

Together, these resources highlight GeneMapper PG’s deterministic, court-ready approach to probabilistic genotyping, empowering forensic labs to build, validate, and defend every interpretation with confidence.

GeneMapper PG Developmental Validation Report

Creating a New STR kit GeneMapper PG Model

Getting started with GeneMapper PG Software

Accelerate your laboratory’s transition to probabilistic genotyping and build confidence in your laboratory’s PG workflows with the GeneMapper PG Software v1.0 Getting Started Guide. From installation to interpretation this comprehensive reference walks through system requirments, configuration, and integration with GeneMapper ID-X Software and explains how to perform Number of Contributors (NOC) estimations, execute deterministic deconvolution, and calculate likelihood ratios (LR).

GeneMapper PG Software v1.0 Getting Started Guide