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Gibco
Mouse PDGF-BB Recombinant Protein
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产品信息
PMG0043
产品规格
Expression System
E. coli
分类
Recombinant
类型
Protein
偶联物
形式
Lyophilized
保存条件
4°C
运输条件
Wet ice
产品详细信息
Carrier-Free
Reconstitution: Centrifuge the vial briefly, before opening to bring the contents to the bottom. Reconstitute the lyophilized protein in 100 mM acetic acid containing 0.1% BSA to 0.1-1.0 mg/mL to regain full activity. Apportion the reconstituted protein into working aliquots and store at =-20°C. Make any further dilutions of the reconstituted protein in low endotoxin medium or buffered solution with FBS or tissue culture grade BSA.
Storage: Store the lyophilized protein at 2-8°C or -20°C for long term storage, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at =-20°C (not in a frost-free freezer). Avoid repeated freeze-thaw cycles.
靶标信息
The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two alternatively spliced transcript variants encoding different isoforms have been identified for this gene.
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