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| 应用 | 建议稀释比 | 已发表文章 |
|---|---|---|
免疫组化 (IHC) |
- | 查看1篇已发表文章 1篇已发表文章 |
免疫组化（石蜡） (IHC (P)) |
1:100 | - |
| 产品规格 | |
|---|---|
种属反应 |
Human |
已发表种属 |
Human |
宿主/亚型 |
Rabbit / IgG |
分类 |
Monoclonal |
类型 |
Antibody |
克隆号 |
SP8 |
抗原 |
Recombinant protein corresponding to a region which spans the tyrosine kinase catalytic domain and part of the C-terminus of the NPM-ALK transcript. |
偶联物 |
Unconjugated |
形式 |
Liquid |
浓度 |
Conc. Not Determined |
保存液 |
tissue culture supernatant diluted in TBS, pH 7.5, with 1% BSA |
内含物 |
<0.1% sodium azide |
保存条件 |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID |
AB_2537839 |
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is anaplastic lymphoma.
The 2;5 chromosomal translocation is frequently associated with anaplastic large cell lymphomas. The translocation creates a fusion gene consisting of the ALK gene and the nucleophosmin gene: the 3' half of ALK, derived from chromosome 2, is fused to the 5' portion of NPM from chromosome 5. A recent study shows that the product of the NPM-ALK fusion gene is oncogenic. The deduced amino acid sequences reveal that ALK is a novel receptor protein-tyrosine kinase having a putative transmembrane domain and an extracellular domain. These sequences are absent in the product of the transforming NPM-ALK gene. ALK shows the greatest sequence similarity to LTK. ALK plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: ALK tyrosine kinase receptor; ALK/NPM1 fusion gene; Anaplastic lymphoma kinase; Anaplastic lymphoma kinase Ki1; Anaplastic Lymphoma Kinase p80; CD246; CD246 antigen; EC 2.7.10.1; kinase ALK; mutant anaplastic lymphoma kinase; npm-alk; TFG/ALK
基因别名: ALK; CD246; NBLST3
UniProt ID: (Human) Q9UM73
Entrez Gene ID: (Human) 238
分子生物学功能:
transmembrane signal receptor
