Invitrogen

Androgen Receptor Monoclonal Antibody (AN1-15)

18 篇参考文献
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产品信息

MA1-150

应用
建议稀释比
已发表文章

免疫印迹 (WB)

1:50-1:500
查看3篇已发表文章 3篇已发表文章

免疫组化 (IHC)

-
查看11篇已发表文章 11篇已发表文章

免疫组化（石蜡） (IHC (P))

2-4 µg/mL
查看1篇已发表文章 1篇已发表文章

免疫组化（冰冻） (IHC (F))

2-4 µg/mL
-

免疫细胞化学 (ICC/IF)

5 µg/mL
查看1篇已发表文章 1篇已发表文章

免疫沉淀 (IP)

Assay-dependent
-

凝胶阻滞分析 (GS)

Assay-dependent
查看2篇已发表文章 2篇已发表文章
产品规格

种属反应

Human, Mouse, Non-human primate, Rat

已发表种属

Human, Mouse, Non-human primate, Rat

宿主/亚型

Rat / IgG

分类

Monoclonal

类型

Antibody

克隆号

AN1-15

抗原

A fusion protein corresponding to residues 331-572 of human androgen receptor.
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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.05% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_325419

产品详细信息

MA1-150 detects androgen receptor from human, Rhesus monkey, rat and mouse tissues. This product does not cross-react with estrogen, progesterone, or glucocorticoid receptors.

MA1-150 has been successfully used in Western blot, immunocytochemistry, immunohistochemistry, gel shift, and immunoprecipitation procedures. By Western blot, this antibody detects an ~110 kDa protein representing androgen receptor in rat tissues. Immunohistochemical staining of androgen receptor in primate hypothalamus with MA1-150 results in intense nuclear staining.

The MA1-150 immunogen is a fusion protein corresponding to residues 331-572 of human androgen receptor.


Reconstitute with PBS.

靶标信息

The androgen receptor (AR) is a member of the steroid-hormone receptor superfamily of nuclear receptors. The receptor is more than 90 kDa and has three major functional domains: the N-terminal domain, DNA-binding domain, and the androgen-binding domain. The androgen receptor is a ligand-activated transcription factor that binds active testosterone (T) and dihydrotestosterone (DHT). Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. The AR signaling pathway plays a key role in development and function of male reproductive organs, including the prostate and epididymis. AR also plays a role in nonreproductive organs, such as muscle, hair follicles, and brain. Androgen Receptor is a phosphoprotein, and also regulates mitogen-activated protein kinase (MAP kinase). The inhibition of the MEK1/2 pathway correlates directly with a change in phosphorylation state of the androgen receptor. Abnormalities in the AR signaling pathway have been linked to a number of diseases, including prostate cancer, Kennedy's disease, and male infertility. Mutations in this gene are associated with complete androgen insensitivity (CAIS).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: androgen nuclear receptor variant 2; Androgen receptor; androgen receptor (Testicular feminization), same as Tfm; androgen receptor splice variant 4b; AR-A; Dihydrotestosterone receptor; Nuclear receptor subfamily 3 group C member 4; RP11-383C12.1; Variant AR45

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基因别名: AIS; Andr; AR; AR8; AW320017; DHTR; HUMARA; HYSP1; KD; NR3C4; SBMA; SMAX1; TFM

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UniProt ID: (Human) P10275, (Rat) P15207, (Mouse) P19091

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Entrez Gene ID: (Human) 367, (Rat) 24208, (Mouse) 11835

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功能
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription factor binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding androgen receptor activity receptor binding steroid binding androgen binding protein binding beta-catenin binding transcription factor binding zinc ion binding enzyme binding transcription regulatory region DNA binding protein dimerization activity ATPase binding protein domain specific binding sequence-specific DNA binding androgen receptor binding POU domain binding
参与通路
transcription, DNA-templated transcription initiation from RNA polymerase II promoter transport signal transduction cell-cell signaling sex differentiation cell proliferation positive regulation of cell proliferation negative regulation of cell proliferation positive regulation of gene expression cell growth androgen receptor signaling pathway intracellular receptor signaling pathway prostate gland development positive regulation of phosphorylation positive regulation of cell differentiation negative regulation of integrin biosynthetic process positive regulation of integrin biosynthetic process positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of transcription from RNA polymerase III promoter positive regulation of NF-kappaB transcription factor activity protein oligomerization regulation of establishment of protein localization to plasma membrane negative regulation of extrinsic apoptotic signaling pathway in utero embryonic development regulation of systemic arterial blood pressure epithelial cell morphogenesis regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter spermatogenesis single fertilization male gonad development fertilization cellular process regulation of gene expression male somatic sex determination response to insulin positive regulation of intracellular estrogen receptor signaling pathway Leydig cell differentiation multicellular organism growth steroid hormone mediated signaling pathway positive regulation of MAPK cascade positive regulation of insulin-like growth factor receptor signaling pathway male sex differentiation reproductive structure development regulation of developmental growth organ formation male genitalia morphogenesis negative regulation of epithelial cell proliferation regulation of catalytic activity activation of prostate induction by androgen receptor signaling pathway morphogenesis of an epithelial fold lateral sprouting involved in mammary gland duct morphogenesis regulation of prostatic bud formation prostate gland growth prostate gland epithelium morphogenesis epithelial cell differentiation involved in prostate gland development tertiary branching involved in mammary gland duct morphogenesis mammary gland alveolus development reproductive system development cellular response to steroid hormone stimulus seminiferous tubule development
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