Invitrogen

Arginase 1 Recombinant Polyclonal Antibody (24HCLC)

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. 查看细节

查看其他31个Arginase 1抗体

说明书

Protocols

技术支持

说明书

Protocols

技术支持

Product Image Gallery

Arginase 1 Antibody in Immunocytochemistry (ICC/IF) — For immunofluorescence analysis, HepG2 cells were fixed and permeabilized for detection of endogenous ARG1 using Anti- ARG1 Recombinant Rabbit Polyclonal Antibody (Product # 711765, 5 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of ARG1 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating cytoplasmic localization of ARG1. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Arginase 1 Antibody in Western Blot (WB) — Western blot analysis was performed on Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), K-562 (Lane 2) and tissue extracts of Mouse Liver (Lane 3). The blots were probed with Anti-ARG1 Recombinant Rabbit Polyclonal Antibody (Product # 711765, 2.5 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 37 kDa band corresponding to ARG1 was observed across the cell lines and tissues tested. Known quantity of protein samples were electrophoresed using Novex®NuPAGE®4-12% Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).

Arginase 1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissue models owing to their inherent genetic constitution. Expression of ARG1 was observed specifically in Mouse Liver in comparison to Mouse Kidney using Anti-ARG1 Recombinant Rabbit Polyclonal Antibody (Product # 711765) in Western blot. {RE}

产品细节

Applications

Tested Dilution

Publications

免疫印迹 (WB)

2.5 µg/mL

免疫细胞化学 (ICC/IF)

5 µg/mL

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Recombinant Polyclonal

类型

Antibody

克隆号

24HCLC

抗原

Peptides corresponding to human Arginase 1 [1) aa304-aa322 2) aa52-aa71]

偶联物

Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2722873

产品详细信息

This antibody is predicted to react with Monkey, Cat, Horse.

Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.

靶标信息

Arginase-1 (Arg1) is a 35 kDa enzyme converting L-arginine to urea and L-ornithine, which is the final step in the urea cycle. The resulting polyamines are important for cell proliferation and removal of toxins that arise from protein degradation. By degrading arginine, Arginase 1 deprives NO synthase of its substrate and down-regulates nitric oxide production. In both human and mouse, Arginase 1 is expressed in the liver, neutrophils, myeloid derived suppressor cells (MDSC) and neural stem cells. In human, expression in blood neutrophils but not in CCR3+ granulocytes has been reported. In mice, expression of Arginase 1 is one of the hallmarks of alternatively activated macrophages (M2a). Arginase-1 may be expressed in the myeloid cells infiltrating tumors, and is typically found in the majority of hepatocellular carcinomas. Defects in Arginase 1 are the cause of argininemia, an autosomal recessive disorder characterized by hyperammonemia.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: A-I; arginase 1 liver; arginase 1, liver; arginase I; arginase, liver; Arginase-1; Arginase1; HGNC:663; Liver Arginase; Liver-type arginase; Type 1 Arginase; Type I arginase

基因别名: AI; AI256583; Arg-1; ARG1; PGIF

UniProt ID: (Human) P05089, (Mouse) Q61176

Entrez Gene ID: (Human) 383, (Mouse) 11846

分子生物学功能: hydrolase