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Invitrogen
BAK1 Monoclonal Antibody (4C2)
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图: 1 / 17
BAK1 Antibody (MA5-36225) in ICC/IF
Immunofluorescence analysis of BAK1 was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.01% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with BAK1 Monoclonal Antibody (4C2) (Product # MA5-36225) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Pr... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
MA5-36225
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Human
宿主/亚型
Mouse
/ IgG2b
分类
Monoclonal
类型
Antibody
克隆号
4C2
抗原
E.coli-derived human BAK/BAK1 recombinant protein (Position: A22-S211).
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
浓度
500 µg/mL
规格
100 µg
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
0.05mg sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human 293T whole cell, human A431 whole cell, human THP-1 whole cell, human MCF-7 whole cell, human PC-3 whole cell. IHC: human intestinal cancer tissue, human mammary cancer tissue, human tonsil tissue, human lung cancer tissue, mouse intestine tissue, rat intestine tissue. ICC/IF: A431 cell. Flow: PC-3 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Apoptosis regulator BAK; ATBAK1; ATSERK3; Bcl-2 homologous antagonist/killer; Bcl-2-like protein 7; Bcl2 homologous antagonist/killer; Bcl2-L-7; BCL2-like 7protein; BRI1-associated receptor kinase; ELG; ELONGATED; F17M5.190; F17M5_190; MGC117255; MGC3887; RECEPTOR KINASES LIKE SERK 10; RKS10; SERK3; SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE 3
基因别名: BAK; BAK1; BCL2L7; CDN1; N-Bak; N-BAK1
UniProt ID: (Mouse) O08734
Entrez Gene ID: (Mouse) 12018, (Rat) 116502
leukocyte homeostasis
B cell homeostasis
B cell apoptotic process
release of cytochrome c from mitochondria
blood vessel remodeling
myeloid cell homeostasis
B cell negative selection
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
mitochondrial fusion
cell proliferation
negative regulation of cell proliferation
intrinsic apoptotic signaling pathway in response to DNA damage
activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c
response to fungus
response to mycotoxin
establishment or maintenance of transmembrane electrochemical gradient
response to gamma radiation
positive regulation of calcium ion transport into cytosol
negative regulation of gene expression
cytolysis
negative regulation of endoplasmic reticulum calcium ion concentration
negative regulation of peptidyl-serine phosphorylation
cellular response to UV
limb morphogenesis
regulation of apoptotic process
positive regulation of apoptotic process
regulation of protein homodimerization activity
regulation of protein heterodimerization activity
fibroblast apoptotic process
positive regulation of proteolysis
regulation of mitochondrial membrane permeability
post-embryonic camera-type eye morphogenesis
homeostasis of number of cells
regulation of cell cycle
regulation of mitochondrial membrane potential
vagina development
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
thymocyte apoptotic process
positive regulation of release of cytochrome c from mitochondria
apoptotic signaling pathway
extrinsic apoptotic signaling pathway in absence of ligand
activation of cysteine-type endopeptidase activity
positive regulation of endoplasmic reticulum unfolded protein response
apoptotic process involved in patterning of blood vessels
brain development
aging
response to UV-C
response to organic cyclic compound
endocrine pancreas development
organ regeneration
response to drug
response to hydrogen peroxide
response to ethanol
cellular response to mechanical stimulus
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