Invitrogen

CD126 Monoclonal Antibody (B-R6), eBioscience™

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CD126 Antibody (BMS135) in Neu

Cell death & disease 2021 - Fig. 6 IL-6 promotes KIAA1199 expression through the PI3K/Akt/NF-kappaB signaling pathway in RA FLS. A - D Relative mRNA expression of KIAA1199 in FLS from healthy subjects ( n = 9) stimulated without (Control) or with 1 and 5 ng/ml TNF-alpha ( A ), IL-1beta ( B ), IL-6 ( C ), and TGF-beta1 ( D ), respectively. E - G Inhibitory effect of IL-6R blocking mAb on the expression of KIAA1199 mRNA ( E ), protein (... View More

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产品信息

BMS135

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

Assay-Dependent

酶联免疫吸附实验 (ELISA)

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中和实验 (Neu)

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功能检测 (Functional)

Assay-Dependent

抑制试剂 (IA)

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

B-R6

抗原

Rec. Human IL-6R (CD126)

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

AB_10598676

产品详细信息

Description: Recognizes soluble and membranous human IL-6R. This Antibody has been shown to inhibit IL-6 mediated proliferation of XG-1 cells, and partially blocks binding of IL-6 to its receptor. BMS135 is applicable for FACS analysis

IL-6 is a multifunctional cytokine involved in the regulation of the immune response, hematopoiesis and acute phase response. It has been recognized to be a member of the alpha-helical cytokine family. IL-6 exerts its action via a cell surface receptor which consists of two subunits, an 80 kDa ligand binding subunit (gp80) of 468 amino acids and a 130 kDa signal transducing protein (gp130) of 896 amino acid residues. Characterisation of the extracellular portion of the 80 kDa IL-6 receptor revealed the existence of a single immunoglobulin-like domain in the NH2-terminal of the extracellular region, which does not contribute to ligand binding. The remainder of the extracellular domain however is essential for low affinity ligand binding, which consecutively triggers the association of the receptor and gp130 thus forming a high affinity binding site for IL-6.

A soluble form of the human gp80 protein has been detected in serum and urine samples. This 55 kDa protein representing the extracellular portion of gp80 is generated by shedding, a process that seems to be controlled by protein kinase C. It is still functional, indicating that soluble gp80 plays a biological role in promoting IL-6 activity. So far, the soluble IL-6 receptor is unique in acting as an agonist together with its ligand.

Applications Tested: Flow Cytometry, Functional Studies (Blocking).

Purity: >95%.

靶标信息

Interleukin 6 receptor (IL6R), also known as CD126 (Cluster of Differentiation 126), is a type I cytokine receptor. This gene encodes a subunit of the interleukin 6 (IL6) receptor complex. Interleukin 6 is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in the immune response. The IL6 receptor is a protein complex consisting of this protein and interleukin 6 signal transducer (IL6ST/GP130/IL6-beta), a receptor subunit also shared by many other cytokines. Dysregulated production of IL6 and this receptor are implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer. Alternatively spliced transcript variants encoding distinct isoforms have been reported. A pseudogene of this gene is found on chromosome 9.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Secreted KIAA1199 promotes the progression of rheumatoid arthritis by mediating hyaluronic acid degradation in an ANXA1-dependent manner.

Cell death & disease

Zhang W,Yin G,Zhao H,Ling H,Xie Z,Xiao C,Chen Y,Lin Y,Jiang T,Jin S,Wang J,Yang X

BMS135 was used in Inhibition assays to elucidate the role and mechanism of sKIAA1199 in extracellular HA degradation and provide in vivo evidence for sKIAA1199’s contribution to RA progression.

Wed Jan 20 00:00:00 UTC 2021

Secreted KIAA1199 promotes the progression of rheumatoid arthritis by mediating hyaluronic acid degradation in an ANXA1-dependent manner.

Cell death & disease

Zhang W,Yin G,Zhao H,Ling H,Xie Z,Xiao C,Chen Y,Lin Y,Jiang T,Jin S,Wang J,Yang X

BMS135 was used in Inhibition assays to elucidate the role and mechanism of sKIAA1199 in extracellular HA degradation and provide in vivo evidence for sKIAA1199’s contribution to RA progression.

Wed Jan 20 00:00:00 UTC 2021

Crohn's Disease Fibroblasts Overproduce the Novel Protein KIAA1199 to Create Proinflammatory Hyaluronan Fragments.

Cellular and molecular gastroenterology and hepatology

Soroosh A,Albeiroti S,West GA,Willard B,Fiocchi C,de la Motte CA

BMS135 was used in Blocking experiments to test the hypothesis that KIAA1199 protein is increased in Crohn''s Disease colon fibroblasts, and generates hyaluronan fragments that foster inflammation and fibrosis.

Sun May 01 00:00:00 UTC 2016

A new strategy to deliver synthetic protein drugs: self-reproducible biologics using minicircles.

Scientific reports

Yi H,Kim Y,Kim J,Jung H,Rim YA,Jung SM,Park SH,Ju JH

BMS135 was used in an ELISA assay to develop a novel strategy to allow the production of synthetic drugs in vivo by the host itself.

Tue Aug 05 00:00:00 UTC 2014

Legionella pneumophila type II secretion dampens the cytokine response of infected macrophages and epithelia.

Infection and immunity

McCoy-Simandle K,Stewart CR,Dao J,DebRoy S,Rossier O,Bryce PJ,Cianciotto NP

BMS135 was used in Blocking experiments to investigate how type II secretion promotes the intracellular infection of both multiple types of macrophages and lung epithelia by Legionella pneumophila.

Sun May 01 00:00:00 UTC 2011

生物信息学

蛋白别名: CD126; CD126 antigen; gp80; IL-6 Receptor; il-6 receptor alpha; IL-6 receptor subunit alpha; IL-6R 1; ILN; Interleukin; Interleukin-6 receptor subunit alpha; Membrane glycoprotein 80; MGC104991

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基因别名: CD126; gp80; IL-6R-1; IL-6RA; IL6Q; IL6R; IL6RA; IL6RQ

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UniProt ID: (Human) P08887

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Entrez Gene ID: (Human) 3570

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功能

ciliary neurotrophic factor receptor activity interleukin-6 receptor activity interleukin-6 receptor binding protein binding enzyme binding interleukin-6 binding protein homodimerization activity ciliary neurotrophic factor binding transmembrane signal receptor

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