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          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • CD14 Antibodies

          Invitrogen

          CD14 Monoclonal Antibody (Sa2-8), Super Bright™ 600, eBioscience™

          9 Published Figures
          4 References
          View all (131) CD14 antibodies
          Datasheet
          Protocols
          Tech Support

          Cite CD14 Monoclonal Antibody (Sa2-8), Super Bright™ 600, eBioscience™

          Datasheet
          Protocols
          Tech Support
          • Antibody Testing Data (1)
          • Published Figures (9)
          CD14 Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 10

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          CD14 Antibody (63-0141-82) in Flow

          BALB/c mouse thioglycolate-elicited peritoneal exudate cells were stained with 0.25 µg of Rat IgG2a kappa Isotype Control, Super Bright 600 (Product # 63-4321-82) (blue histogram) or 0.25 µg of CD14 Monoclonal Antibody, Super Bright 600 (purple histogram). Total viable cells were used for analysis, as determined by 7-AAD (Product # 00-6993-50). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          Product Details

          63-0141-82

          Applications
          Tested Dilution
          Publications

          免疫细胞化学 (ICC/IF)

          -
          查看1篇已发表文章 1篇已发表文章

          流式细胞分析 (Flow)

          0.5 µg/test
          查看3篇已发表文章 3篇已发表文章
          产品规格

          Species Reactivity

          Mouse

          Published species

          Not Applicable

          Host/Isotype

          Rat / IgG2a, kappa

          Recommended Isotype Control

          Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 600, eBioscience™

          分类

          Monoclonal

          类型

          Antibody

          克隆号

          Sa2-8

          Conjugate

          Super Bright™ 600
          • APC 
          • Biotin 
          • FITC
          • PE 
          • PE-Cyanine7
          • PerCP-Cyanine5.5
          • PerCP-eFluor 710
          • Super Bright 436
          • Super Bright 645

          Excitation/Emission Max

          414/601 nm View spectra spectra

          形式

          Liquid

          浓度

          0.2 mg/mL

          纯化类型

          Affinity chromatography

          保存液

          PBS, pH 7.2, with BSA

          内含物

          0.09% sodium azide

          保存条件

          4° C, store in dark, DO NOT FREEZE!

          RRID

          AB_2762769

          Product Specific Information

          Description: The Sa2-8 monoclonal antibody reacts with mouse CD14, a 53-55 kDa GPI-linked glycoprotein. CD14 is a receptor for the complexes of LPS and LBP (LPS-Binding Protein) and is shown to associate with Toll-Like Receptor 4 (TLR4) and participate in the signaling and cellular response to bacterial LPS. In mouse, CD14 is expressed on the surface of macrophages and under certain conditions is also found in the serum in a secreted form. Sa2-8 has weak antagonistic activity (in NF-kappaB activation or TNF alpha production with LPS stimulation).

          Applications Reported: This Sa2-8 antibody has been reported for use in flow cytometric analysis.

          Applications Tested: This Sa2-8 antibody has been tested by flow cytometric analysis of thioglycolate-elicited peritoneal macrophages. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

          When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

          Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

          Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

          Excitation: 405 nm; Emission: 600 nm; Laser: Violet Laser

          Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

          Target Information

          CD14 is a 55 kDa GPI-anchored glycoprotein that is constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils. CD14 also serves as a multifunctional lipopolysaccharide receptor, and is released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 can discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. Further, CD14 has been shown to bind apoptotic cells, and can affect allergic, inflammatory and infectious processes. Alternative splicing results in multiple transcript variants encoding the same CD14 isoform. Diseases associated with CD14 dysfunction include mycobacterium chelonae infection and Croup.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          Flow Cytometry
          Panel Builder

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          Bioinformatics

          Protein Aliases: CD 14; CD14; cd14 monocyte; LPSR antibody; Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; sCD14; soluble CD14

          View more View less

          Gene Aliases: Cd14

          View more View less

          UniProt ID: (Mouse) P10810

          View more View less

          Entrez Gene ID: (Mouse) 12475

          View more View less

          Molecular Function: transmembrane signal receptor

          Function(s)
          response to molecule of bacterial origin immune system process inflammatory response positive regulation of tumor necrosis factor production response to tumor necrosis factor innate immune response positive regulation of endocytosis positive regulation of cytokine secretion cellular response to lipopolysaccharide cellular response to lipoteichoic acid
          Process(es)
          lipopolysaccharide binding lipoteichoic acid binding
          It has to be done as per old AB suggested Products section.
          guarantee_icon

          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

          Learn more
          help_icon

          We're here to help

          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

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