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Invitrogen
CD146 Monoclonal Antibody (P1H12), Alexa Fluor™ 488
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CD146 Antibody (MA562202) in Flow
Flow cytometry (surface staining) of CD146 in HUVEC cells. Samples were stained with CD146 monoclonal antibody (Product # MA562202) using a dilution of 5 µg/mL. Separation of stained HUVEC cells (red-filled) from HUVEC cells stained using mouse IgG1 isotype control (MOPC-21) Alexa Fluor® 488 antibody (5 µg/mL, black-dashed).
产品信息
MA562202
应用
建议稀释比
已发表文章
产品规格
种属反应
Dog,
Human,
Mouse,
Rabbit
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
P1H12
抗原
Cultured human umbilical cells.
偶联物
激发/发射光谱
499/520 nm
查看光谱
形式
Liquid
浓度
0.13 mg/mL
纯化类型
Size-exclusion chromatography
保存液
PBS, pH 7.4, with 0.2% BSA
内含物
15mM sodium azide
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
靶标信息
CD146, also known as MCAM (Melanoma Cell Adhesion Molecule), is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. It is heavily glycosylated, with more than 50% of its mass derived from carbohydrates. CD146 is primarily expressed on endothelial cells at cell-cell junctions, playing a crucial role in cell adhesion and the cohesion of the endothelial monolayer in vascular tissue. In addition to endothelial cells, CD146 is expressed on bone marrow fibroblasts, mesenchymal stromal cells, endometrial stromal cells, and some tumors, particularly melanoma. It has also been observed on a subset of circulating T cells and activated lymphocytes, but not on circulating endothelial cells, as indicated by the absence of other endothelial markers such as CD31 and CD51/61. CD146 functions as a calcium-independent cell adhesion molecule and may also act as a signal transduction molecule. It is involved in the recruitment of Fyn kinase and the subsequent tyrosine phosphorylation of intracellular proteins, which are important for actin cytoskeleton assembly. This signaling role includes triggering tyrosine phosphorylation of FYN and PTK2 and causing a transient increase in intracellular calcium concentration. In the context of cancer, CD146 expression may facilitate the interaction of melanoma cells with the vascular system, enhancing hematogenous tumor spread. Additionally, CD146 could serve as an adhesion molecule active in neural crest cells during embryonic development, highlighting its diverse roles in both normal physiological processes and disease states.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CD146; Cell surface glycoprotein MUC18; Cell surface glycoprotein P1H12; Gicerin; l-gicerin protein; melanoma adhesion molecule; Melanoma cell adhesion molecule; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; S-endo 1 endothelial-associated antigen
基因别名: 1-gicerin; AV025631; CD146; CD149; MCAM; MUC18; s-endo; s-gicerin
UniProt ID: (Human) P43121, (Mouse) Q8R2Y2
Entrez Gene ID: (Rabbit) 100342848, (Human) 4162, (Dog) 489368, (Mouse) 84004
