Invitrogen

CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH5), Functional Grade, eBioscience™

Product Image Gallery

CD274 (PD-L1, B7-H1) Antibody in Flow Cytometry (Flow) — Staining of mouse splenocytes with Anti-Mouse CD274 (B7-H1) PE Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

CD274 (PD-L1, B7-H1) Antibody in Immunocytochemistry (ICC/IF) — Figure 6 LCMV induces the strongest tumour regression compared with oncolytic viruses and PD1 blockade. ( a , b ) Tumour diameter and survival of MOPC-tumour-bearing C57BL/6 mice (day -10) treated intratumourally ( a ) or intravenously ( b ) with LCMV, VSV-GP, rVACV or vehicle ( n =5 per group). ( c ) Tumour diameter and survival of Sw480-tumour-bearing NOD/SCID mice treated i.t. with 2 x 10 6 PFU of LCMV, rVACV, VSV-GP or MOCK control ( n =5 per group). ( d ) Immunofluorescence of tumours from MOPC-tumour-bearing mice (day -10) treated with or without 2 x 10 4 PFU LCMV peritumourally on day 0 (LCMV, green; red PD-L1; n =3 per group). ( e ) Tumour diameter and survival of MOPC-tumour-bearing C57BL/6 and Pdcd1 -/- mice (day -10) treated with or without 2 x 10 4 PFU LCMV peritumourally on day 0 ( n =5 per group). Data are shown as mean+-s.e.m. and analysed by unpaired Student's t -test. Survival is shown in Kaplan-Meier method and analysed by log-rank test. NS, nonsignificant; * P <0.05, ** P <0.01 and *** P <0.001; scale bar, 200 mum.

CD274 (PD-L1, B7-H1) Antibody in Immunohistochemistry (IHC) — Figure 5. Downregulation of PD-L1 and JAK expression inhibits migration and EMT of C4-2-Sc and CWR22Rv1-Sc cells. C4-2-Sc and CWR22Rv1-Sc cells were treated with PD-L1 antibodies and JAK inhibitor 1. (A) Migration of tumor cells was significantly decreased following incubation with PD-L1 antibodies and JAK inhibitor 1. Magnification, x40. (B) Expression of EMT-associated markers was reversed, and EMT of the tumor cells was inhibited. One-way analysis of variance followed by Fisher's least significant difference post hoc test was used for comparison among groups. ***P<0.001. ATM, ataxia telangiectasia mutated kinase; E-Cad, E-cadherin; EMT, epithelial-mesenchymal transition; JAK, Janus kinase; N-Cad, N-cadherin; PD-L1, programmed cell death 1 ligand 1; Sc, scramble; si, small interfering RNA; VEGF, vascular endothelial growth factor.

CD274 (PD-L1, B7-H1) Antibody in Western Blot (WB) — Figure 4 Generation of FoxA1 + T reg cells requires neuronal FoxA1 and PDL1. ( a ) Percentage of FoxA1 + T regs upon co-culture of T encs with CGNs, with or without transwell. Graphs are mean+-s.e.m., n =3. One-way analysis of variance (ANOVA) test was used, *** P <0.001. ( b ) Fold change of Foxa1 mRNA and ( c ) Pdl1 mRNA in CGNs treated with or without rIFNbeta (100 U ml -1 ). Graphs are mean+-s.e.m., n =3, two-way ANOVA test was used, * P <0.05 and ** P <0.01. ( d ) Foxa1 and ( e ) Pdl1 mRNA in CGNs (left) and quantification (right). Graphs are mean+-s.e.m., n =3, Student's t -test was used, *** P <0.001. ( f ) Cytosolic (Cyto) and nucleus (Nu) fractions from CN (left). Graph shows normalized optical density (IOD) ratios between cytosolic/nucleus fractions of quantified FoxA1 protein bands (right). Graphs are mean+-s.e.m., n =2-4. Non-parametric Mann-Whitney test was used for comparison of treated groups with control group. * P <0.05 and ** P <0.01. ( g ) CGNs with or without treatment with rIFNbeta. NF200 (green), FoxA1 (red) and DAPI (blue). Scale bars, 5 mum. ( h ) WB of FoxA1, PDL1 and vinculin in CNs extracts with or without treatment with rIFNbeta. ( i ) Representative FACS histogram of neuronal FoxA1 and PDL1 after Foxa1 siRNAKD in CGNs and ( j ) WB of FoxA1, PDL1 and vinculin after Foxa1 siRNAKD in CGNs. ( k ) Percentage of FoxA1 + T regs after co-culture of T encs with CGNs after Foxa1 or control siRNAKD in CGNs. Graphs are mean+-s.e.m., n =3, Student's

CD274 (PD-L1, B7-H1) Antibody in Neutralization (Neu) — Figure 3 Evaluation of the effects of N-cad on the expression of PD-L1 and IDO-1 (five mice). (A) EMT and neuroendocrine (NE) phenotypes in N-cad-overexpressing cells and N-cad knockout (N-cad KO) cells. We used the LNCap, PC3 and PC3 vector (scrambled smallinterfering RNA) cells as the control. (B) PD-L1 and IDO-1 expression in N-cad-overexpressing cells and N-cad KO cells after treatment with IFN-gamma. PC3 cells were used as a positive control. (C) Changes in the levels of N-cad in cell lines after treatment with IFN-gamma. (D) Changes in the expression of PD-L1, IDO-1 and EMT markers after treatment with kynurenine. (E) Changes in the levels of EMT markers after treatment with the PD-L1 neutralizing antibody (""-"" means PD-L1 block, blank means use PBS as blank control). (F) IHC staining for PD-L1 in different mice (n=5) tumors; the black bars in the IHC image are 200 um. (G) IHC staining for IDO-1 in different mouse (n=5) tumors; the black bars in the IHC image are 200 um. (H) H&E staining in mouse tissues; the black bars in the IHC image are 200 um. AR, androgen receptor; Cg-A, Chromogranin-A; EMT, epithelial-mesenchymaltransition; IDO-1, indole amine 2,3-dioxygenase; IFN-gamma, interferon gamma; IHC, immunohistochemistry; IL-8, interleukin-8; N-cad, N-cadherin; NSE, neuron-specific enolase; PBS, phosphate-bufferedsaline; PD-L1, programmed death ligand-1.

CD274 (PD-L1, B7-H1) Antibody in Functional assay (FN) — Fig. 3 CD273 and CD274 enhance contact between Treg and macrophages. (A) Purified Treg and macrophages were co-cultured in the absence (1) or presence of isotype control (2), anti-CD273 (3), or anti-CD273 and anti-CD274 antibodies (4). Macrophages are generally bigger in size under a microscope than Treg. Treg are fluorescence labeled with Cell Trace FR shown in pseudo green to distinguish from macrophages. Each black dot represents one interaction from a randomly chosen Treg and each cell may encounter one or multiple interactions. The contact time between Treg and macrophages was analyzed using video recordings with fluorescence and phase contrast microscopy. Contact time above 400 s above the dotted red line was regarded as a meaningful interaction and graphed against total contact time as a percentage. Scale bars = 10 um. The data shown are representative of six independent experiments. (B) Collated data of percentage of cells with long interactions (> 400 s) are shown with the mean +- SD (error bars). (C) Collated data of the contact time from (A) with the mean +- SD (error bars). Student's t -test was performed and and P < 0.05 was considered statistically significant (ns, not significant; *P < 0.05, ** P < 0.01).

产品信息

16-5982-82

应用	建议稀释比	已发表文章
免疫印迹 (WB)	-	查看1篇已发表文章 1篇已发表文章
免疫组化 (IHC)	-	查看2篇已发表文章 2篇已发表文章
免疫细胞化学 (ICC/IF)	-	查看1篇已发表文章 1篇已发表文章
流式细胞分析 (Flow)	0.5 µg/test	查看15篇已发表文章 15篇已发表文章
中和实验 (Neu)	Assay-Dependent	查看9篇已发表文章 9篇已发表文章
功能检测 (FN)	Assay-Dependent	查看3篇已发表文章 3篇已发表文章
抑制试剂 (IA)	-	查看3篇已发表文章 3篇已发表文章
体外检测 (IV)	-	查看1篇已发表文章 1篇已发表文章

产品规格
种属反应	Mouse
已发表种属	Human, Mouse
宿主/亚型	Rat / IgG2a, lambda
推荐的同型对照	Rat IgG2a kappa Isotype Control (eBR2a), Functional Grade, eBioscience™
分类	Monoclonal
类型	Antibody
克隆号	MIH5
偶联物	Functional Grade Alexa Fluor 488 Alexa Fluor 660 APC Biotin Brilliant UV 737 Brilliant UV 805 PE PE-Cyanine7 PerCP-eFluor 710 Super Bright 436 Super Bright 600 Super Bright 645 Super Bright 780 Unconjugated
形式	Liquid
浓度	1 mg/mL
纯化类型	Affinity chromatography
保存液	PBS, pH 7.2
内含物	no preservative
保存条件	4° C
RRID	AB_469178

产品详细信息

Description: The MIH5 monoclonal antibody reacts with mouse B7-H1, also known as PD-L1. B7-H1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily. B7-H1 is expressed on a majority of leukocytes including T, B, NK and DC. B7-H1 is a ligand for PD-1. Interaction of PD-1 with either PD-L1 (B7-H1) or PD-L2 (B7-DC) results in inhibition of T and B cell responses. MIH5 is reported to be a blocking antibody.

Applications Reported: The MIH5 antibody has been reported for use in flow cytometric analysis. It has also been reported in blocking in in vitro and in vivo functional assays.

Applications Tested: The MIH5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

靶标信息

Programmed death receptor ligand 1 (PD-L1, also called B7-H1) is a recently described B7 family member. To date, one specific receptor has been identified that can be ligated by PD-L1. This receptor, programmed death receptor 1 (PD-1), has been shown to negatively regulate T-cell receptor (TCR) signaling. Upon ligating its receptor, PD-L1 has been reported to decrease TCR-mediated proliferation and cytokine production. PD-L1 expression was found to be abundant on many murine and human cancers and could be further up-regulated upon IFN-gamma stimulation. Thus, PD-L1 might play an important role in tumor immune evasion.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

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Mon Mar 01 00:00:00 EST 2021

Progranulin induces immune escape in breast cancer via up-regulating PD-L1 expression on tumor-associated macrophages (TAMs) and promoting CD8⁺ T cell exclusion.

Journal of experimental & clinical cancer research : CR

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16-5982-82 was used in Functional assay to provide new ideas and targets for clinical breast cancer immunotherapy.

Mon Jan 04 00:00:00 EST 2021

Progranulin induces immune escape in breast cancer via up-regulating PD-L1 expression on tumor-associated macrophages (TAMs) and promoting CD8⁺ T cell exclusion.

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Fang W,Zhou T,Shi H,Yao M,Zhang D,Qian H,Zeng Q,Wang Y,Jin F,Chai C,Chen T

16-5982-82 was used in Functional assay to provide new ideas and targets for clinical breast cancer immunotherapy.

Mon Jan 04 00:00:00 EST 2021

CD279 mediates the homeostasis and survival of regulatory T cells by enhancing T cell and macrophage interactions.

FEBS open bio

Liu Y,Gao Y,Hao H,Hou T

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Mon Jun 01 00:00:00 EDT 2020

Downregulation of PD-L1 via amide analogues of brefelamide: Alternatives to antibody-based cancer immunotherapy.

Experimental and therapeutic medicine

Zhang J,Yamada O,Kida S,Murase S,Hattori T,Oshima Y,Kikuchi H

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 16-5982-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2020

Cancer cell-intrinsic expression of MHC II in lung cancer cell lines is actively restricted by MEK/ERK signaling and epigenetic mechanisms.

Journal for immunotherapy of cancer

Neuwelt AJ,Kimball AK,Johnson AM,Arnold BW,Bullock BL,Kaspar RE,Kleczko EK,Kwak JW,Wu MH,Heasley LE,Doebele RC,Li HY,Nemenoff RA,Clambey ET

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Wed Apr 01 00:00:00 EDT 2020

MicroRNA-200a induces immunosuppression by promoting PTEN-mediated PD-L1 upregulation in osteosarcoma.

Aging

Liu Z,Wen J,Wu C,Hu C,Wang J,Bao Q,Wang H,Wang J,Zhou Q,Wei L,Shen Y,Zhang W

16-5982 was used in Flow cytometry/Cell sorting to provide important and novel insight into a regulatory axis involving microRNA-200a/PTEN/ PD-L1 axis, which determines osteosarcoma growth and the efficacy of PD-L1-targeted immunotherapy.

Fri Jan 24 00:00:00 EST 2020

Tumor-intrinsic response to IFNγ shapes the tumor microenvironment and anti-PD-1 response in NSCLC.

Life science alliance

Bullock BL,Kimball AK,Poczobutt JM,Neuwelt AJ,Li HY,Johnson AM,Kwak JW,Kleczko EK,Kaspar RE,Wagner EK,Hopp K,Schenk EL,Weiser-Evans MC,Clambey ET,Nemenoff RA

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 16-5982-82) in Flow Cytometry

Sat Jun 01 00:00:00 EDT 2019

Inhibition of ATM reverses EMT and decreases metastatic potential of cisplatin-resistant lung cancer cells through JAK/STAT3/PD-L1 pathway.

Journal of experimental & clinical cancer research : CR

Shen M,Xu Z,Xu W,Jiang K,Zhang F,Ding Q,Xu Z,Chen Y

16-5982-82 was used in Inhibition Assays to investigate the effects of Ataxia Telangiectasia Mutated (ATM) on lung cancer metastasis.

Mon Apr 08 00:00:00 EDT 2019

Inhibition of ATM reverses EMT and decreases metastatic potential of cisplatin-resistant lung cancer cells through JAK/STAT3/PD-L1 pathway.

Journal of experimental & clinical cancer research : CR

Shen M,Xu Z,Xu W,Jiang K,Zhang F,Ding Q,Xu Z,Chen Y

16-5982-82 was used in Inhibition Assays to investigate the effects of Ataxia Telangiectasia Mutated (ATM) on lung cancer metastasis.

Mon Apr 08 00:00:00 EDT 2019

The antitumor effects of entinostat in ovarian cancer require adaptive immunity.

Cancer

Smith HJ,McCaw TR,Londono AI,Katre AA,Meza-Perez S,Yang ES,Forero A,Buchsbaum DJ,Randall TD,Straughn JM,Norian LA,Arend RC

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Sat Dec 15 00:00:00 EST 2018

ATM‑JAK‑PD‑L1 signaling pathway inhibition decreases EMT and metastasis of androgen‑independent prostate cancer.

Molecular medicine reports

Zhang L,Xu LJ,Zhu J,Li J,Xue BX,Gao J,Sun CY,Zang YC,Zhou YB,Yang DR,Shan YX

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Tue May 01 00:00:00 EDT 2018

PD-L1 in tumor microenvironment mediates resistance to oncolytic immunotherapy.

The Journal of clinical investigation

Zamarin D,Ricca JM,Sadekova S,Oseledchyk A,Yu Y,Blumenschein WM,Wong J,Gigoux M,Merghoub T,Wolchok JD

Published figure using CD274 (PD-L1, B7-H1) monoclonal antibody (Product # 16-5982-82) in Flow Cytometry

Mon Apr 02 00:00:00 EDT 2018

Oncofetal gene SALL4 reactivation by hepatitis B virus counteracts miR-200c in PD-L1-induced T cell exhaustion.

Nature communications

Sun C,Lan P,Han Q,Huang M,Zhang Z,Xu G,Song J,Wang J,Wei H,Zhang J,Sun R,Zhang C,Tian Z

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Wed Mar 28 00:00:00 EDT 2018

Oncogenic RAS Signaling Promotes Tumor Immunoresistance by Stabilizing PD-L1 mRNA.

Immunity

Coelho MA,de Carné Trécesson S,Rana S,Zecchin D,Moore C,Molina-Arcas M,East P,Spencer-Dene B,Nye E,Barnouin K,Snijders AP,Lai WS,Blackshear PJ,Downward J

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Tue Dec 19 00:00:00 EST 2017

Homeostatic control of metabolic and functional fitness of T_reg cells by LKB1 signalling.

Nature

Yang K,Blanco DB,Neale G,Vogel P,Avila J,Clish CB,Wu C,Shrestha S,Rankin S,Long L,Kc A,Chi H

16-5982 was used in Inhibition experiments to show that liver kinase B1 programs the metabolic and functional fitness of Treg cells in the control of immune tolerance and homeostasis.

Thu Aug 31 00:00:00 EDT 2017

Preclinical Development of Ipilimumab and Nivolumab Combination Immunotherapy: Mouse Tumor Models, In Vitro Functional Studies, and Cynomolgus Macaque Toxicology.

PloS one

Selby MJ,Engelhardt JJ,Johnston RJ,Lu LS,Han M,Thudium K,Yao D,Quigley M,Valle J,Wang C,Chen B,Cardarelli PM,Blanset D,Korman AJ

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Fri Aug 25 00:00:00 EDT 2017

Neuronal IFN-beta-induced PI3K/Akt-FoxA1 signalling is essential for generation of FoxA1⁺T_reg cells.

Nature communications

Liu Y,Marin A,Ejlerskov P,Rasmussen LM,Prinz M,Issazadeh-Navikas S

16-5982 was used in Neutralization experiments to investigate the role of IFN in generation of FoxA1(+)Treg cells.

Mon Apr 24 00:00:00 EDT 2017

Neuronal IFN-beta-induced PI3K/Akt-FoxA1 signalling is essential for generation of FoxA1⁺T_reg cells.

Nature communications

Liu Y,Marin A,Ejlerskov P,Rasmussen LM,Prinz M,Issazadeh-Navikas S

16-5982 was used in Neutralization experiments to investigate the role of IFN in generation of FoxA1(+)Treg cells.

Mon Apr 24 00:00:00 EDT 2017

Spatiotemporally restricted arenavirus replication induces immune surveillance and type I interferon-dependent tumour regression.

Nature communications

Kalkavan H,Sharma P,Kasper S,Helfrich I,Pandyra AA,Gassa A,Virchow I,Flatz L,Brandenburg T,Namineni S,Heikenwalder M,Höchst B,Knolle PA,Wollmann G,von Laer D,Drexler I,Rathbun J,Cannon PM,Scheu S,Bauer J,Chauhan J,Häussinger D,Willimsky G,Löhning M,Schadendorf D,Brandau S,Schuler M,Lang PA,Lang KS

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Wed Mar 01 00:00:00 EST 2017

Exploring the induction of preproinsulin-specific Foxp3(+) CD4(+) Treg cells that inhibit CD8(+) T cell-mediated autoimmune diabetes by DNA vaccination.

Scientific reports

Stifter K,Schuster C,Schlosser M,Boehm BO,Schirmbeck R

16-5982 was used in Blocking experiments to demonstrate that vaccine-induced Treg cells play a role in control of autoreactive effector CD8(+) T-cells in a diabetes model.

Mon Jul 11 00:00:00 EDT 2016

Exploring the induction of preproinsulin-specific Foxp3(+) CD4(+) Treg cells that inhibit CD8(+) T cell-mediated autoimmune diabetes by DNA vaccination.

Scientific reports

Stifter K,Schuster C,Schlosser M,Boehm BO,Schirmbeck R

16-5982 was used in Blocking experiments to demonstrate that vaccine-induced Treg cells play a role in control of autoreactive effector CD8(+) T-cells in a diabetes model.

Mon Jul 11 00:00:00 EDT 2016

Maternal-Derived Hepatitis B Virus e Antigen Alters Macrophage Function in Offspring to Drive Viral Persistence after Vertical Transmission.

Immunity

Tian Y,Kuo CF,Akbari O,Ou JH

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Tue May 17 00:00:00 EDT 2016

Recruited monocytes modulate malaria-induced lung injury through CD36-mediated clearance of sequestered infected erythrocytes.

Journal of leukocyte biology

Lagassé HA,Anidi IU,Craig JM,Limjunyawong N,Poupore AK,Mitzner W,Scott AL

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Targeting immune co-stimulatory effects of PD-L1 and PD-L2 might represent an effective therapeutic strategy in stroke.

Frontiers in cellular neuroscience

Bodhankar S,Chen Y,Lapato A,Vandenbark AA,Murphy SJ,Offner H

16-5982 was used in Flow cytometry/Cell sorting to investigate PD-L1 and PD-L2 expressing cell types that affect T-cell activation post stroke, and how these could be targeted therapeutically.

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PD-L1 is a novel direct target of HIF-1α, and its blockade under hypoxia enhanced MDSC-mediated T cell activation.

The Journal of experimental medicine

Noman MZ,Desantis G,Janji B,Hasmim M,Karray S,Dessen P,Bronte V,Chouaib S

16-5982 was used in Neutralization experiments to determine that blockage of the HRE within the proximal promoter of PD-1 ligand causes preferential anticancer MDSC determination.

Mon May 05 00:00:00 EDT 2014

A mechanism of hypoxia-mediated escape from adaptive immunity in cancer cells.

Cancer research

Barsoum IB,Smallwood CA,Siemens DR,Graham CH

16-5982 was used in Blocking experiments to investigate whether exposure of mouse and human tumour cells to hypoxia increases their resistance to T cell-mediated immunity.

Sat Feb 01 00:00:00 EST 2014

Farnesyltransferase inhibitor FTI-277 reduces mortality of septic mice along with improved bacterial clearance.

The Journal of pharmacology and experimental therapeutics

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Ex vivo expanded hematopoietic stem cells overcome the MHC barrier in allogeneic transplantation.

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Zheng J,Umikawa M,Zhang S,Huynh H,Silvany R,Chen BP,Chen L,Zhang CC

Fri Aug 05 00:00:00 EDT 2011

Ex vivo expanded hematopoietic stem cells overcome the MHC barrier in allogeneic transplantation.

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Zheng J,Umikawa M,Zhang S,Huynh H,Silvany R,Chen BP,Chen L,Zhang CC

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Control of established melanoma by CD27 stimulation is associated with enhanced effector function and persistence, and reduced PD-1 expression of tumor infiltrating CD8(+) T cells.

Journal of immunotherapy (Hagerstown, Md. : 1997)

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Fri Oct 01 00:00:00 EDT 2010

Control of established melanoma by CD27 stimulation is associated with enhanced effector function and persistence, and reduced PD-1 expression of tumor infiltrating CD8(+) T cells.

Journal of immunotherapy (Hagerstown, Md. : 1997)

Roberts DJ,Franklin NA,Kingeter LM,Yagita H,Tutt AL,Glennie MJ,Bullock TN

16-5982 was used in in vitro experiments to examine the efficacy of agonistic anti-CD27 antibodies as monotherapies for established melanoma in a murine model.

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Antigen-specific immunity and cross-priming by epithelial ovarian carcinoma-induced CD11b(+)Gr-1(+) cells.

Journal of immunology (Baltimore, Md. : 1950)

Tomihara K,Guo M,Shin T,Sun X,Ludwig SM,Brumlik MJ,Zhang B,Curiel TJ,Shin T

16-5982 was used in Blocking experiments to investigate CD11b(+)Gr-1(+) cells found in epithelial ovarian cancer-bearing mice, which are induced by ovarian cancer cells and undergo antigen-specific immunity and cross-priming.

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A critical role of IFNgamma in priming MSC-mediated suppression of T cell proliferation through up-regulation of B7-H1.

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Sheng H,Wang Y,Jin Y,Zhang Q,Zhang Y,Wang L,Shen B,Yin S,Liu W,Cui L,Li N

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Murine isolated lymphoid follicles contain follicular B lymphocytes with a mucosal phenotype.

American journal of physiology. Gastrointestinal and liver physiology

Wang C,McDonald KG,McDonough JS,Newberry RD

16-5982 was used in Flow cytometry/Cell sorting to indicate that murine ILFs contain a polyclonal population of follicular B-2 B lymphocytes with a phenotype similar to PP B lymphocytes.

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生物信息学

蛋白别名: B7 homolog 1; B7-H1; CD274; PD-L1; PDCD1 ligand 1; pdl-1; Programmed cell death 1 ligand 1; programmed death ligand 1; sCD274; soluble CD274; soluble PD L1; sPD L1

基因别名: A530045L16Rik; B7h1; Cd274; Pdcd1l1; Pdcd1lg1; Pdl1

UniProt ID: (Mouse) Q9EP73

Entrez Gene ID: (Mouse) 60533

分子生物学功能: immunoglobulin receptor superfamily

功能

immune response signal transduction cell surface receptor signaling pathway positive regulation of cell migration T cell costimulation negative regulation of interferon-gamma production negative regulation of interleukin-10 production positive regulation of T cell proliferation negative regulation of T cell proliferation negative regulation of activated T cell proliferation toxin transport negative regulation of tumor necrosis factor superfamily cytokine production positive regulation of interleukin-10 secretion

参与通路

protein binding

CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH5), Functional Grade, eBioscience™

产品信息

16-5982-82

免疫印迹 (WB)

免疫组化 (IHC)

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

中和实验 (Neu)

功能检测 (FN)

抑制试剂 (IA)

体外检测 (IV)

种属反应

已发表种属

宿主/亚型

推荐的同型对照

分类

类型

克隆号

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

RRID

产品详细信息

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生物信息学

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