Invitrogen

CD4 Monoclonal Antibody (RM4-5), Super Bright™ 645, eBioscience™

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

63 Published Figures

53 References

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CD4 Antibody (64-0042-82) in Flow

Staining of C57BL/6 splenocytes with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and Rat IgG2a K Isotype Control (Product # 64-4321-82) (left) or 0.125 µg of Anti-Mouse CD4 Super Bright 645 (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

CD4 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow) — Figure 1. MHC II expression on IECs is required for DP IEL differentiation. (A) Representative plots (left) and MHC expression (right; mean +- SEM) on IECs (CD45.2 - EpCAM + gated) in each small intestine segment, duodenum (d), jejunum (j), and ileum (i) of C57BL/6 mice ( n = 6). Expression level is shown as mean fluorescence intensity (MFI). The data shown are representative of six independent experiments. (B) Significantly enriched GO terms of DEGs among IECs sorted from d, j, and i. (C) Heatmap of MHC II-related gene expression in IECs from d, j, and i of the small intestine. The same numbers under d, j, and i correspond to the replicates from the same mouse. (D) GSEA using gene sets of MHC class II biosynthesis (GO:0045342) and antigen processing and presentation via MHC class II (GO:0002504) for duodenal and ileal IECs. ES, enrichment score; NES, normalized enrichment score. (E) Representative plots (left) and DP IEL frequency (right; TCRbeta + CD4 + gated; mean +- SEM) in each small intestine segment of C57BL/6 mice ( n = 6). The gating strategy for DP IELs is shown in Fig. S1 F . The data shown are representative of six independent experiments. (F) Correlation analysis between epithelial MHC II expression and DP IEL frequency by linear regression fit in each intestinal segment ( n = 29). Analysis was performed on pooled data of six independent experiments. R, correlation coefficient; P, significance of the slope. (G) DP IEL frequency in MHC II fl/fl and MHC II ^IEC mic

CD4 Antibody in Immunocytochemistry (ICC/IF) — Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Immunocytochemistry

CD4 Antibody in Immunocytochemistry, Immunohistochemistry (PFA fixed) (ICC/IF, IHC (PFA)) — Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Immunocytochemistry and Immunohistochemistry (PFA fixed)

CD4 Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Figure 4 Expression of IL-16 by infiltrating immune cells in EAE mice. Spinal cord and brains were harvested from EAE and PBS control mice. Tissue was co-stained with IL-16 (green) and CD45, CD4, or F4/80 (all red). Representative images of spinal cord ( A - C ), cerebellum ( D ) and hippocampus ( E ) tissues are shown. Dotted white lines indicate spinal cord section edges. White arrows indicate areas of colocalization. Percentage of double-positive cells in each group within the spinal cord ( A - C ), hippocampus ( F , G ) and cerebellum ( H , I ) were quantified with results expressed as the mean +- SEM. N = 5. Statistical significance was determined by one-way ANOVA with Bonferroni post hoc tests. * p < 0.05, ** p < 0.01, *** p < 0.001 versus PBS or comparison groups are indicated by the bar lines.

CD4 Antibody in Immunohistochemistry (IHC) — Regulatory T-cell recruitment to HNSCC tumor sites is inhibited by dietary black raspberry extract administration. (A) Multiplex confocal microscopy images of representative tongues taken from each mouse group. For each image, individual stains and composite images are shown. Each tongue was stained for DAPI (blue), CD4 (red), and FoxP3 (green). FoxP3 + CD4 + cells were quantified using ImageJ. (B) Western blot demonstrating FoxP3, and GAPDH protein content of tongue lysates isolated from sentinel, 4NQO, and 4NQO+BRB-E groups. Average protein intensity of Western blot bands was measured for several samples using ImageJ. (C) RT-qPCR data of tongue homogenate taken from sentinel or cancer-bearing mice receiving the control diet or the black raspberry extract supplemented feed for Foxp3, Il2r , and Ctla4 . Data are represented as fold change relative to the Bactin control. * p -value < 0.05; ** p -value < 0.01; *** p -value < 0.001; # p -value < 0.1 for comparisons of BRB-E treatment groups to the 4NQO-exposed control diet group using Student's t -test.

CD4 Antibody — Staining of mouse splenocytes and bone marrow cells. Right: As expected based on known relative expression patterns, CD4 clone RM4-5 stains a subset of splenocytes and does not stain any bone marrow cells. Details: Balb/c bone marrow cells (left) and splenocytes (middle) were surface stained with CD4 (clone RM4-5) followed by staining with 7-AAD. Viable bone marrow cells in the lymphoid (blue histogram) and myeloid (purple histogram) gates and viable splenocytes (orange histogram) were used for analysis. {RE}

Product Details

64-0042-82

Applications

Tested Dilution

Publications

免疫组化 (IHC)

查看6篇已发表文章 6篇已发表文章

免疫组织化学（多聚甲醛固定） (IHC (PFA))

查看1篇已发表文章 1篇已发表文章

免疫组化（冰冻） (IHC (F))

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免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

0.25 µg/test

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产品规格

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 645, eBioscience™

分类

Monoclonal

类型

Antibody

克隆号

RM4-5

Conjugate

Super Bright™ 645

Excitation/Emission Max

414/645 nm View spectra

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

RRID

AB_2662401

Product Specific Information

Description: The RM4-5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of RM4-5 is blocked by GK1.5.

Applications Reported: The RM4-5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The RM4-5 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm. We recommend using a 660/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 405 nm; Emission: 645 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Flow Cytometry

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Product References

Impact of broad-spectrum antibiotics on the gut-microbiota-spleen-brain axis.

Brain, behavior, & immunity - health

Wan X,Eguchi A,Sakamoto A,Fujita Y,Yang Y,Qu Y,Hatano M,Mori C,Hashimoto K

Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2023

Klebsiella pneumoniae Induces Inflammatory Bowel Disease Through Caspase-11-Mediated IL18 in the Gut Epithelial Cells.

Cellular and molecular gastroenterology and hepatology

Zhang Q,Su X,Zhang C,Chen W,Wang Y,Yang X,Liu D,Zhang Y,Yang R

Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Flow Cytometry

Thu Feb 23 00:00:00 EST 2023

Aryl hydrocarbon receptor activation ameliorates experimental colitis by modulating the tolerogenic dendritic and regulatory T cell formation.

Cell & bioscience

Cui X,Ye Z,Wang D,Yang Y,Jiao C,Ma J,Tang N,Zhang H

Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Flow Cytometry

Sat Apr 23 00:00:00 EDT 2022

IL-33 induces thymic involution-associated naive T cell aging and impairs host control of severe infection.

Nature communications

Xu L,Wei C,Chen Y,Wu Y,Shou X,Chen W,Lu D,Sun H,Li W,Yu B,Wang X,Zhang X,Yu Y,Lei Z,Tang R,Zhu J,Li Y,Lu L,Zhou H,Zhou S,Su C,Chen X

Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Flow Cytometry

Sat Nov 12 00:00:00 EST 2022

Fringe GlcNAc-transferases differentially extend O-fucose on endogenous NOTCH1 in mouse activated T cells.

The Journal of biological chemistry

Matsumoto K,Kumar V,Varshney S,Nairn AV,Ito A,Pennarubia F,Moremen KW,Stanley P,Haltiwanger RS

Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Flow Cytometry

Fri Jul 01 00:00:00 EDT 2022

Breast cancer cell-derived extracellular vesicles promote CD8⁺ T cell exhaustion via TGF-β type II receptor signaling.

Nature communications

Xie F,Zhou X,Su P,Li H,Tu Y,Du J,Pan C,Wei X,Zheng M,Jin K,Miao L,Wang C,Meng X,van Dam H,Ten Dijke P,Zhang L,Zhou F

Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2022

Black raspberry extract inhibits regulatory T-cell activity in a murine model of head and neck squamous cell carcinoma chemoprevention.

Frontiers in immunology

Ryan NM,Lamenza FF,Upadhaya P,Pracha H,Springer A,Swingler M,Siddiqui A,Oghumu S

Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Immunohistochemistry

Mon Aug 29 00:00:00 EDT 2022

Niche-specific MHC II and PD-L1 regulate CD4+CD8αα+ intraepithelial lymphocyte differentiation.

The Journal of experimental medicine

Moon S,Park Y,Hyeon S,Kim YM,Kim JH,Kim H,Park S,Lee KJ,Koo BK,Ha SJ,Lee SW

Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Flow Cytometry

Mon Apr 05 00:00:00 EDT 2021

Niche-specific MHC II and PD-L1 regulate CD4+CD8αα+ intraepithelial lymphocyte differentiation.

The Journal of experimental medicine

Moon S,Park Y,Hyeon S,Kim YM,Kim JH,Kim H,Park S,Lee KJ,Koo BK,Ha SJ,Lee SW

Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Flow Cytometry

Mon Apr 05 00:00:00 EDT 2021

Romidepsin (FK228) regulates the expression of the immune checkpoint ligand PD-L1 and suppresses cellular immune functions in colon cancer.

Cancer immunology, immunotherapy : CII

Shi Y,Fu Y,Zhang X,Zhao G,Yao Y,Guo Y,Ma G,Bai S,Li H

Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

Potential therapeutic effects of interleukin-35 on the differentiation of naïve T cells into Helios⁺Foxp3⁺ Tregs in clinical and experimental acute respiratory distress syndrome.

Molecular immunology

Wang C,Xie K,Li K,Lin S,Xu F

Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

Feline adipose tissue-derived mesenchymal stem cells pretreated with IFN-γ enhance immunomodulatory effects through the PGE₂ pathway.

Journal of veterinary science

Park SG,An JH,Li Q,Chae HK,Park SM,Lee JH,Ahn JO,Song WJ,Youn HY

Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Flow Cytometry

Mon Mar 01 00:00:00 EST 2021

Imbalance between T helper 1 and regulatory T cells plays a detrimental role in experimental Parkinson's disease in mice.

The Journal of international medical research

Li W,Luo Y,Xu H,Ma Q,Yao Q

Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

Intratumoral expression of interleukin 23 variants using oncolytic vaccinia virus elicit potent antitumor effects on multiple tumor models via tumor microenvironment modulation.

Theranostics

Chen L,Chen H,Ye J,Ge Y,Wang H,Dai E,Ren J,Liu W,Ma C,Ju S,Guo ZS,Liu Z,Bartlett DL

Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Flow Cytometry

Wed Aug 04 00:00:00 EDT 2021

Tissue plasminogen activator worsens experimental autoimmune encephalomyelitis by complementary actions on lymphoid and myeloid cell responses.

Journal of neuroinflammation

Hélie P,Camacho-Toledano C,Lesec L,Seillier C,Miralles AJ,Ortega MC,Guérit S,Lebas H,Bardou I,Vila-Del Sol V,Vivien D,Le Mauff B,Clemente D,Docagne F,Toutirais O

Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Immunohistochemistry

Sat Feb 20 00:00:00 EST 2021

Increased Levels of IL-16 in the Central Nervous System during Neuroinflammation Are Associated with Infiltrating Immune Cells and Resident Glial Cells.

Biology

Hridi SU,Barbour M,Wilson C,Franssen AJ,Harte T,Bushell TJ,Jiang HR

Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Immunohistochemistry (Frozen)

Thu May 27 00:00:00 EDT 2021

Epithelial Mutant p53 Promotes Resistance to Anti-PD-1-Mediated Oral Cancer Immunoprevention in Carcinogen-Induced Mouse Models.

Cancers

Wang J,Hu Y,Escamilla-Rivera V,Gonzalez CL,Tang L,Wang B,El-Naggar AK,Myers JN,Caulin C

Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Immunohistochemistry

Tue Mar 23 00:00:00 EDT 2021

Histological, behavioural and flow cytometric datasets relating to acute ischaemic stroke in young, aged and ApoE^-/- mice in the presence and absence of immunomodulation with fingolimod.

Data in brief

Diaz Diaz AC,Malone K,Shearer JA,Moore AC,Waeber C

Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Flow Cytometry

Tue Jun 01 00:00:00 EDT 2021

Near-infrared photoimmunotherapy targeting human-EGFR in a mouse tumor model simulating current and future clinical trials.

EBioMedicine

Okada R,Furusawa A,Vermeer DW,Inagaki F,Wakiyama H,Kato T,Nagaya T,Choyke PL,Spanos WC,Allen CT,Kobayashi H

Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Flow Cytometry

Sat May 01 00:00:00 EDT 2021

Activation of Bone Marrow Adaptive Immunity in Type 2 Diabetes: Rescue by Co-stimulation Modulator Abatacept.

Frontiers in immunology

Santopaolo M,Sullivan N,Thomas AC,Alvino VV,Nicholson LB,Gu Y,Spinetti G,Kallikourdis M,Blom A,Madeddu P

Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Flow Cytometry

Fri Jul 02 00:00:00 EDT 2021

Evaluation of DNA-Launched Virus-Like Particle Vaccines in an Immune Competent Mouse Model of Chikungunya Virus Infection.

Vaccines

Rayner JO,Kim JH,Roberts RW,Wood RR,Fouty B,Solodushko V

Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Flow Cytometry

Fri Apr 02 00:00:00 EDT 2021

Engineered macrophages as near-infrared light activated drug vectors for chemo-photodynamic therapy of primary and bone metastatic breast cancer.

Nature communications

Huang Y,Guan Z,Dai X,Shen Y,Wei Q,Ren L,Jiang J,Xiao Z,Jiang Y,Liu D,Huang Z,Xu X,Luo Y,Zhao C

Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Flow Cytometry

Wed Jul 14 00:00:00 EDT 2021

Gut Microbiota Dysbiosis Induced by Intracerebral Hemorrhage Aggravates Neuroinflammation in Mice.

Frontiers in microbiology

Yu X,Zhou G,Shao B,Zhou H,Xu C,Yan F,Wang L,Chen G,Li J,Fu X

Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Flow Cytometry

Wed May 26 00:00:00 EDT 2021

Antitumor efficacy of a neoantigen cancer vaccine delivered by electroporation is influenced by microbiota composition.

Oncoimmunology

Lione L,Salvatori E,Petrazzuolo A,Massacci A,Maggio R,Confroti A,Compagnone M,Aurisicchio L,Ciliberto G,Palombo F

Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Flow Cytometry

Tue Mar 23 00:00:00 EDT 2021

Local Sustained GM-CSF Delivery by Genetically Engineered Encapsulated Cells Enhanced Both Cellular and Humoral SARS-CoV-2 Spike-Specific Immune Response in an Experimental Murine Spike DNA Vaccination Model.

Vaccines

Vernet R,Charrier E,Cosset E,Fièvre S,Tomasello U,Grogg J,Mach N

64-0042-82 was used in Flow Cytometry to investigate whether murine Granulocyte-macrophage colony-stimulating factor (GM-CSF) represents a suitable adjuvant for SARS-CoV-2 immunization.

Mon May 10 00:00:00 EDT 2021

Serum Amyloid A Proteins Induce Pathogenic Th17 Cells and Promote Inflammatory Disease.

Cell

Lee JY,Hall JA,Kroehling L,Wu L,Najar T,Nguyen HH,Lin WY,Yeung ST,Silva HM,Li D,Hine A,Loke P,Hudesman D,Martin JC,Kenigsberg E,Merad M,Khanna KM,Littman DR

Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Immunocytochemistry

Thu Jan 09 00:00:00 EST 2020

Protein arginine methyltransferase 5 promotes cholesterol biosynthesis-mediated Th17 responses and autoimmunity.

The Journal of clinical investigation

Webb LM,Sengupta S,Edell C,Piedra-Quintero ZL,Amici SA,Miranda JN,Bevins M,Kennemer A,Laliotis G,Tsichlis PN,Guerau-de-Arellano M

Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2020

DKK2 blockage-mediated immunotherapy enhances anti-angiogenic therapy of Kras mutated colorectal cancer.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Hu J,Wang Z,Chen Z,Li A,Sun J,Zheng M,Wu J,Shen T,Qiao J,Li L,Li B,Wu D,Xiao Q

Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2020

Comparison of rapamycin and methylprednisolone for treating inflammatory muscle disease in a murine model of experimental autoimmune myositis.

Experimental and therapeutic medicine

Kang J,Feng D,Yang F,Tian X,Han W,Jia H

Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2020

Effects of CD4⁺ T lymphocytes from ovariectomized mice on bone marrow mesenchymal stem cell proliferation and osteogenic differentiation.

Experimental and therapeutic medicine

Shao BY,Wang L,Yu Y,Chen L,Gan N,Huang WM

Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Flow Cytometry

Sun Nov 01 00:00:00 EDT 2020

Dietary Glucose Consumption Promotes RALDH Activity in Small Intestinal CD103⁺CD11b⁺ Dendritic Cells.

Frontiers in immunology

Ko HJ,Hong SW,Verma R,Jung J,Lee M,Kim N,Kim D,Surh CD,Kim KS,Rudra D,Im SH

Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Flow Cytometry

Mon Apr 19 00:00:00 EDT 2021

The lysophospholipase D enzyme Gdpd3 is required to maintain chronic myelogenous leukaemia stem cells.

Nature communications

Naka K,Ochiai R,Matsubara E,Kondo C,Yang KM,Hoshii T,Araki M,Araki K,Sotomaru Y,Sasaki K,Mitani K,Kim DW,Ooshima A,Kim SJ

Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Flow Cytometry

Thu Sep 17 00:00:00 EDT 2020

Oncolytic vaccinia virus delivering tethered IL-12 enhances antitumor effects with improved safety.

Journal for immunotherapy of cancer

Ge Y,Wang H,Ren J,Liu W,Chen L,Chen H,Ye J,Dai E,Ma C,Ju S,Guo ZS,Liu Z,Bartlett DL

Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Flow Cytometry

Sun Mar 01 00:00:00 EST 2020

A novel antibody targeting TIM-3 resulting in receptor internalization for cancer immunotherapy.

Antibody therapeutics

Kuang Z,Li L,Zhang P,Chen B,Wu M,Ni H,Yi S,Zou J,Liu J

Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2020

Angioimmunoblastic T-cell lymphoma-like lymphadenopathy in mice transgenic for human RHOA with p.Gly17Val mutation.

Oncoimmunology

Lee GJ,Jun Y,Yoo HY,Jeon YK,Lee D,Lee S,Kim J

Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Immunohistochemistry

Wed May 13 00:00:00 EDT 2020

Mechanisms of interleukin 4 mediated increase in efficacy of vaccines against opioid use disorders.

NPJ vaccines

Crouse B,Robinson C,Huseby Kelcher A,Laudenbach M,Abrahante JE,Pravetoni M

Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Flow Cytometry

Thu Oct 29 00:00:00 EDT 2020

Distinct actions of the fermented beverage kefir on host behaviour, immunity and microbiome gut-brain modules in the mouse.

Microbiome

van de Wouw M,Walsh AM,Crispie F,van Leuven L,Lyte JM,Boehme M,Clarke G,Dinan TG,Cotter PD,Cryan JF

Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Flow Cytometry

Mon May 18 00:00:00 EDT 2020

Senolytics prevent mt-DNA-induced inflammation and promote the survival of aged organs following transplantation.

Nature communications

Iske J,Seyda M,Heinbokel T,Maenosono R,Minami K,Nian Y,Quante M,Falk CS,Azuma H,Martin F,Passos JF,Niemann CU,Tchkonia T,Kirkland JL,Elkhal A,Tullius SG

Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Flow Cytometry

Thu Aug 27 00:00:00 EDT 2020

Loss of testosterone impairs anti-tumor neutrophil function.

Nature communications

Markman JL,Porritt RA,Wakita D,Lane ME,Martinon D,Noval Rivas M,Luu M,Posadas EM,Crother TR,Arditi M

Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Flow Cytometry

Tue Mar 31 00:00:00 EDT 2020

Positive Effects of Preventive Nutrition Supplement on Anticancer Radiotherapy in Lung Cancer Bearing Mice.

Cancers

Liu YM,Wu TH,Chiu YH,Wang H,Li TL,Hsia S,Chan YL,Wu CJ

Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Immunohistochemistry

Fri Aug 28 00:00:00 EDT 2020

Prodrug-Loaded Zirconium Carbide Nanosheets as a Novel Biophotonic Nanoplatform for Effective Treatment of Cancer.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Liu Q,Xie Z,Qiu M,Shim I,Yang Y,Xie S,Yang Q,Wang D,Chen S,Fan T,Ding B,Guo Z,Adah D,Yao X,Zhang Y,Wu H,Wu Z,Wei C,Wang H,Kim HS,Zou Q,Yan Q,Cai Z,Kim JS,Liu LP,Zhang H,Cao Y

Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Immunohistochemistry

Tue Dec 01 00:00:00 EST 2020

T Cell Recruitment to the Intestinal Stem Cell Compartment Drives Immune-Mediated Intestinal Damage after Allogeneic Transplantation.

Immunity

Fu YY,Egorova A,Sobieski C,Kuttiyara J,Calafiore M,Takashima S,Clevers H,Hanash AM

Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Immunocytochemistry

Tue Jul 16 00:00:00 EDT 2019

T Cell Recruitment to the Intestinal Stem Cell Compartment Drives Immune-Mediated Intestinal Damage after Allogeneic Transplantation.

Immunity

Fu YY,Egorova A,Sobieski C,Kuttiyara J,Calafiore M,Takashima S,Clevers H,Hanash AM

Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Immunocytochemistry

Tue Jul 16 00:00:00 EDT 2019

Chloroquine modulates inflammatory autoimmune responses through Nurr1 in autoimmune diseases.

Scientific reports

Park TY,Jang Y,Kim W,Shin J,Toh HT,Kim CH,Yoon HS,Leblanc P,Kim KS

Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Flow Cytometry

Tue Oct 29 00:00:00 EDT 2019

Innate Sensing through Mesenchymal TLR4/MyD88 Signals Promotes Spontaneous Intestinal Tumorigenesis.

Cell reports

Koliaraki V,Chalkidi N,Henriques A,Tzaferis C,Polykratis A,Waisman A,Muller W,Hackam DJ,Pasparakis M,Kollias G

Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Flow Cytometry

Tue Jan 15 00:00:00 EST 2019

Mitophagy in Intestinal Epithelial Cells Triggers Adaptive Immunity during Tumorigenesis.

Cell

Ziegler PK,Bollrath J,Pallangyo CK,Matsutani T,Canli Ö,De Oliveira T,Diamanti MA,Müller N,Gamrekelashvili J,Putoczki T,Horst D,Mankan AK,Öner MG,Müller S,Müller-Höcker J,Kirchner T,Slotta-Huspenina J,Taketo MM,Reinheckel T,Dröse S,Larner AC,Wels WS,Ernst M,Greten TF,Arkan MC,Korn T,Wirth D,Greten FR

Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Flow Cytometry

Thu Jun 28 00:00:00 EDT 2018

TCF-1 and HEB cooperate to establish the epigenetic and transcription profiles of CD4⁺CD8⁺ thymocytes.

Nature immunology

Emmanuel AO,Arnovitz S,Haghi L,Mathur PS,Mondal S,Quandt J,Okoreeh MK,Maienschein-Cline M,Khazaie K,Dose M,Gounari F

Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Flow Cytometry

Sat Dec 01 00:00:00 EST 2018

Macrophage-specific MHCII expression is regulated by a remote Ciita enhancer controlled by NFAT5.

The Journal of experimental medicine

Buxadé M,Huerga Encabo H,Riera-Borrull M,Quintana-Gallardo L,López-Cotarelo P,Tellechea M,Martínez-Martínez S,Redondo JM,Martín-Caballero J,Flores JM,Bosch E,Rodríguez-Fernández JL,Aramburu J,López-Rodríguez C

Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Flow Cytometry

Mon Nov 05 00:00:00 EST 2018

Runx-dependent and silencer-independent repression of a maturation enhancer in the Cd4 gene.

Nature communications

Kojo S,Yasmin N,Muroi S,Tenno M,Taniuchi I

Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Flow Cytometry

Wed Sep 05 00:00:00 EDT 2018

IL-36 Signaling Facilitates Activation of the NLRP3 Inflammasome and IL-23/IL-17 Axis in Renal Inflammation and Fibrosis.

Journal of the American Society of Nephrology : JASN

Chi HH,Hua KF,Lin YC,Chu CL,Hsieh CY,Hsu YJ,Ka SM,Tsai YL,Liu FC,Chen A

Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Flow Cytometry

Sat Jul 01 00:00:00 EDT 2017

Enhanced T-cell activation and differentiation in lymphocytes from transgenic mice expressing ubiquitination-resistant 2KR LAT molecules.

Gene therapy

Rodriguez-Peña AB,Gomez-Rodriguez J,Kortum RL,Palmer DC,Yu Z,Guittard GC,Wohlfert EA,Silver PB,Misplon JA,Sommers CL,Feigenbaum L,Epstein SL,Caspi RR,Belkaid Y,Restifo NP,Samelson LE,Balagopalan L

Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2015

Near-infrared photoactivatable control of Ca(2+) signaling and optogenetic immunomodulation.

eLife

He L,Zhang Y,Ma G,Tan P,Li Z,Zang S,Wu X,Jing J,Fang S,Zhou L,Wang Y,Huang Y,Hogan PG,Han G,Zhou Y

Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Flow Cytometry

Tue Dec 08 00:00:00 EST 2015

P2X7 receptor modulates inflammatory and functional pulmonary changes induced by silica.

PloS one

Monção-Ribeiro LC,Faffe DS,Santana PT,Vieira FS,da Graça CL,Marques-da-Silva C,Machado MN,Caruso-Neves C,Zin WA,Borojevic R,Takiya CM,Coutinho-Silva R

Published figure using CD4 monoclonal antibody (Product # 64-0042-82) in Flow Cytometry

Thu Sep 17 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: CD4; CD4 antigen p55; cd4a; fCD4; Leu-3; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4)

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Gene Aliases: Cd4; L3T4; Ly-4

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UniProt ID: (Mouse) P06332

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Entrez Gene ID: (Mouse) 12504

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Function(s)

cytokine production immune system process induction by virus of host cell-cell fusion immune response cell adhesion cell surface receptor signaling pathway T cell differentiation maintenance of protein location in cell T cell activation T cell selection protein palmitoleylation positive regulation of protein kinase activity positive regulation of peptidyl-tyrosine phosphorylation defense response to Gram-negative bacterium positive regulation of calcium-mediated signaling regulation of T cell activation positive regulation of T cell activation

Process(es)

glycoprotein binding protein binding zinc ion binding coreceptor activity enzyme binding protein kinase binding protein homodimerization activity

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CD4 Monoclonal Antibody (RM4-5), Super Bright™ 645, eBioscience™

CD4 Antibody (64-0042-82) in Flow

Product Details

64-0042-82

免疫组化 (IHC)

免疫组织化学（多聚甲醛固定） (IHC (PFA))

免疫组化（冰冻） (IHC (F))

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

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