Invitrogen

CD45 Monoclonal Antibody (HI30), Qdot™ 655

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CD45 Antibody (Q22154) in Flow

Histogram overlay plot of gated human lymphocytes, the black line represents cells labeled with CD45 Mouse Anti-Human mAb (clone HI30), Qdot® 655 Conjugate and the gray line represents unstained cells. Samples were acquired and analyzed using 405 nm excitation and a 660/20 band pass emission filter using the Attune® Acoustic Cytometer Blue/Violet option.

Product Image Gallery

CD45 Antibody — Antibody clone (HI30) specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Lossof signal was observed for target protein in HI30 KOcells (blue histogram) compared to the control Cas9cells (pink histogram) using CD45 antibody (HI30). The Yellow histogram represents staining with the isotype control. {KO}

产品信息

Q22154

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

Assay-dependent

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其他PubMed (Misc)

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

HI30

抗原

CD45 antigen

偶联物

Qdot™ 655 Qdot™ 655 Qdot™ 655

激发/发射光谱

300/654 nm 查看光谱

形式

Liquid

纯化类型

purified

保存液

0.05M borate, pH 8.3, with 1M betaine

内含物

0.05% sodium azide

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2556510

靶标信息

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Dynamic change in natural killer cell type in the human ocular mucosa in situ as means of immune evasion by adenovirus infection.

Mucosal immunology

Yawata N,Selva KJ,Liu YC,Tan KP,Lee AW,Siak J,Lan W,Vania M,Arundhati A,Tong L,Li J,Mehta JS,Yawata M

Q22154 was used in flow cytometry to investigate the dynamics and characteristics of natural killer cell types in the human ocular mucosal surface in situ during infection with group D human adenoviruses.

Fri Jan 01 00:00:00 UTC 2016

Transcriptional regulation of Munc13-4 expression in cytotoxic lymphocytes is disrupted by an intronic mutation associated with a primary immunodeficiency.

The Journal of experimental medicine

Cichocki F,Schlums H,Li H,Stache V,Holmes T,Lenvik TR,Chiang SC,Miller JS,Meeths M,Anderson SK,Bryceson YT

Q22154 was used in flow cytometry to find Munc13-4 is highly expressed in differentiated human natural killer cells and effector CD8(+) T lymphocytes

Mon Jun 02 00:00:00 UTC 2014

Telomere attrition occurs during ex vivo expansion of human dental pulp stem cells.

Journal of biomedicine & biotechnology

Mokry J,Soukup T,Micuda S,Karbanova J,Visek B,Brcakova E,Suchanek J,Bouchal J,Vokurkova D,Ivancakova R

Q22154 was used in flow cytometry to characterize stem cells isolated and expanded from the human dental pulp

Wed May 18 00:00:00 UTC 2011

生物信息学

蛋白别名: CD45; CD45 antigen; L-CA; Leukocyte common antigen; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen; T220 and B220

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基因别名: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; PTPRC; T200

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UniProt ID: (Human) P08575

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Entrez Gene ID: (Human) 5788

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功能

protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding protein kinase binding protein phosphatase protein modifying enzyme

参与通路