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Invitrogen
CD62L (L-Selectin) Monoclonal Antibody (DREG.55), eBioscience™
产品信息
BMS121
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
DREG.55
抗原
Rec. Human CD62L (L-selectin)
偶联物
形式
Liquid
浓度
0.1 mg/mL
纯化类型
Protein G
保存液
PBS, pH 7.2-7.4
内含物
no preservative
保存条件
4° C
运输条件
Wet ice
RRID
AB_10609216
产品详细信息
Description: The antibody can be used to stain acetone-fixed cryostat sections or cell smears. BMS121 is not suitable for paraffin sections but is suitable as primary antibody in staining for FACS analysis.
BMS121FI can be used for direct staining of cells, BMS121BT can be used in a 2-step procedure using the biotin-(strept)avidin system.
BMS121 has not been tested in functional assays but is suitable for ELISA application.
Leukocyte-Endothelial Cell Adhesion Molecule-1 belongs to the selectin family of adhesion molecule. Together with E-selectin, P-selectin and L-selectin mediates the initial interactions of leukocytes with endothelial cells.
Selectins guide non-activated polymorphonuclear cells to the areas of inflammation in creating first, loose contacts with the endothelial layer. L-selectin in this aspect mediates rolling of PMN's on endothelial cells. The potential binding partners of L-selectin carry a negative charge, probably a sialic acid and/or sulphate, and may contain mannose and fucose. In addition, L-selectin may also interact with E-selectin which is expressed on cytokine-activated endothelial cells. L-selectin is constitutively expressed on most leukocytes in a seemingly functional form. It is required for the binding of lymphocytes to the high endothelial venules of peripheral lymph nodes and for the invasion of neutrophils into sites of inflammation.
When neutrophils are activated, L-selectin is shed by proteolytic cleveage near the transmembrane span. Lymphocytes and monocytes can also shed L-selectin upon activation although the kinetics are significantly lower. A broad range of activating agents are effective in inducing this response. The shed form of sL-selectin is functionally active and at high concentrations can inhibit leukocyte attachment to endothelium. The main source for sL-selectin in serum seems to be tissue localized leukocytes.
Applications Tested: ELISA, Flow Cytometry, Immunohistochemistry (Frozen).
Purity: >95%.
靶标信息
The human CD62L is a 74-95 kDa glycoprotein member of the selectin family of adhesion receptors. L-Selectin is comprised of an aminoterminal C-type lectin binding domain, an epidermal growth factor-like domain, two short consensus repeat (SCR) sequences homologous to those found in complement binding proteins, a short spacer region, a transmembrane region and a short cytoplasmic region. Human CD62L (L-Selectin) is constitutively expressed on all classes of leukocytes including lymphocytes (except a substantial population of memory T cells), monocytes and polymorphonuclear cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CD62 antigen-like family member L; CD62L; gp90-MEL; hLHRc; L-selectin; LAM-1; LECAM1; Leu-8; Leukocyte adhesion molecule 1; Leukocyte surface antigen Leu-8; Leukocyte-endothelial cell adhesion molecule 1; Lymph node homing receptor; lymphocyte adhesion molecule 1; pln homing receptor; TQ1
基因别名: CD62L; LAM1; LECAM1; LEU8; LNHR; LSEL; LYAM1; PLNHR; SELL; TQ1
UniProt ID: (Human) P14151
Entrez Gene ID: (Human) 6402
