Invitrogen

CD8 alpha Monoclonal Antibody (5H10), Pacific Blue™

CD8 alpha Antibody in Flow Cytometry (Flow)

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CD8 alpha Antibody (MCD0828TR) in Flow

Flow Cytometry analysis using a CD8 alpha monoclonal antibody (Product # MCD0828).

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产品信息

MCD0828TR

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

Assay-dependent

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其他PubMed (Misc)

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

5H10

抗原

Con A-stimulated BALB/c splenic T cells

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偶联物

Pacific Blue™ Pacific Blue™ Pacific Blue™

激发/发射光谱

404/455 nm 查看光谱

形式

Liquid

纯化类型

purified

保存液

PBS with BSA

内含物

0.1% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2539693

靶标信息

Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

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Product References

Platelet factors attenuate inflammation and rescue cognition in ageing.

Nature

Schroer AB,Ventura PB,Sucharov J,Misra R,Chui MKK,Bieri G,Horowitz AM,Smith LK,Encabo K,Tenggara I,Couthouis J,Gross JD,Chan JM,Luke A,Villeda SA

MCD0828 was used in Flow cytometry/Cell sorting to show that platelet factors transfer the benefits of young blood to the ageing brain.

Tue Aug 01 00:00:00 EDT 2023

Histological, behavioural and flow cytometric datasets relating to acute ischaemic stroke in young, aged and ApoE^-/- mice in the presence and absence of immunomodulation with fingolimod.

Data in brief

Diaz Diaz AC,Malone K,Shearer JA,Moore AC,Waeber C

MCD0828 was used in Flow cytometry/Cell sorting to assess the sphingosine-1-phosphate receptor modulator fingolimod as a preclinical candidate for the treatment of acute ischaemic stroke according to the Stroke Therapy Academic Industry Roundtable (STAIR) preclinical recommendations.

Tue Jun 01 00:00:00 EDT 2021

Histological, behavioural and flow cytometric datasets relating to acute ischaemic stroke in young, aged and ApoE^-/- mice in the presence and absence of immunomodulation with fingolimod.

Data in brief

Diaz Diaz AC,Malone K,Shearer JA,Moore AC,Waeber C

Tue Jun 01 00:00:00 EDT 2021

Assessing IL-2-Induced STAT5 Phosphorylation in Fixed, Permeabilized Foxp3⁺ Treg Cells by Multiparameter Flow Cytometry.

STAR protocols

Li C,Park JH

MCD0828 was used in Flow cytometry/Cell sorting to reveal the cytokine responsiveness of individual T cells.

Fri Dec 18 00:00:00 EST 2020

Trans-omics Impact of Thymoproteasome in Cortical Thymic Epithelial Cells.

Cell reports

Ohigashi I,Tanaka Y,Kondo K,Fujimori S,Kondo H,Palin AC,Hoffmann V,Kozai M,Matsushita Y,Uda S,Motosugi R,Hamazaki J,Kubota H,Murata S,Tanaka K,Katagiri T,Kosako H,Takahama Y

Published figure using CD8 alpha monoclonal antibody (Product # MCD0828) in Flow Cytometry

Tue Nov 26 00:00:00 EST 2019

Survival of Naïve T Cells Requires the Expression of Let-7 miRNAs.

Frontiers in immunology

Pobezinskaya EL,Wells AC,Angelou CC,Fagerberg E,Aral E,Iverson E,Kimura MY,Pobezinsky LA

MCD0828 was used in Flow cytometry/Cell sorting to study the role of let-7miRNA in peripheral naive T-cell homeostasis.

Mon Oct 19 00:00:00 EDT 2020

Mutation of an L-Type Calcium Channel Gene Leads to T Lymphocyte Dysfunction.

Frontiers in immunology

Fenninger F,Han J,Stanwood SR,Nohara LL,Arora H,Choi KB,Munro L,Pfeifer CG,Shanina I,Horwitz MS,Jefferies WA

MCD0828TR was used in Flow cytometry/Cell sorting to investigate how a mutation in an L-Type calcium channel gene influences T lymphocyte dysfunction.

Thu Oct 29 00:00:00 EDT 2020

Therapeutic antiviral T cells noncytopathically clear persistently infected microglia after conversion into antigen-presenting cells.

The Journal of experimental medicine

Herz J,Johnson KR,McGavern DB

MCD0828 was used in flow cytometry to determine if therapeutic antiviral T cells eliminate viral load in the brain of mice persistently infected from birth with lymphocytic choriomeningitis virus without causing blood-brain barrier breakdown or tissue damage

Mon Jul 27 00:00:00 EDT 2015

Continuous Antigenic Stimulation of DO11.10 TCR Transgenic Mice in the Presence or Absence of IL-1β: Possible Implications for Mechanisms of T Cell Depletion in HIV Disease.

Journal of immunology (Baltimore, Md. : 1950)

Ladell K,Hazenberg MD,Fitch M,Emson C,McEvoy-Hein Asgarian BK,Mold JE,Miller C,Busch R,Price DA,Hellerstein MK,McCune JM

MCD0828 was used in flow cytometry to explore the impact of chronic antigenic stimulation on CD4(+) T cell dynamics.

Sun Nov 01 00:00:00 EDT 2015

PD-1 promotes immune exhaustion by inducing antiviral T cell motility paralysis.

The Journal of experimental medicine

Zinselmeyer BH,Heydari S,Sacristán C,Nayak D,Cammer M,Herz J,Cheng X,Davis SJ,Dustin ML,McGavern DB

MCD0828 was used in flow cytometry to study immune exhaustion

Mon Apr 08 00:00:00 EDT 2013

The location of splenic NKT cells favours their rapid activation by blood-borne antigen.

The EMBO journal

Barral P,Sánchez-Niño MD,van Rooijen N,Cerundolo V,Batista FD

MCD0828 was used in flow cytometry to study splenic natural killer T cells

Wed May 16 00:00:00 EDT 2012

生物信息学

蛋白别名: CD8a; CD8alpha; CD8b; CD8beta; fCD8; Leu-2; leu-2a; Lymphocyte antigen 3; Lyt-2.1 lymphocyte differentiation antigen (AA at 100); T-cell membrane glycoprotein Ly-3; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-cell surface glycoprotein Lyt-2; T-cell surface glycoprotein Lyt-3

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基因别名: BB154331; Cd8a; Cd8b; Cd8b1; Ly-2; Ly-3; Ly-35; Ly-B; Ly-C; Lyt-2; Lyt-3; Lyt2; Lyt3

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UniProt ID: (Mouse) P01731, (Mouse) P10300

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Entrez Gene ID: (Mouse) 12525, (Mouse) 12526

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功能

protein binding protein kinase binding protein homodimerization activity defense/immunity protein immunoglobulin receptor superfamily

参与通路

immune system process T cell mediated immunity cell surface receptor signaling pathway T cell activation cytotoxic T cell differentiation positive regulation of calcium-mediated signaling defense response to virus