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Invitrogen
CHRNA1 Monoclonal Antibody (88B)
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CHRNA1 Antibody (MA3-043) in ICC/IF
Immunofluorescent analysis of Nicotinic Acetylcholine Receptor using Anti-Nicotinic Acetylcholine Receptor Monoclonal Antibody (88B) (Product # MA3-043) shows staining in Neuro-2a Cells. Nicotinic Acetylcholine Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Nicotinic Acetylcholine Receptor (Product # MA3-043) at a dilution of 1:100 o... View More
产品信息
MA3-043
应用
建议稀释比
已发表文章
产品规格
种属反应
Amphibian,
Chicken,
Fish,
Human,
Mouse,
Rat
已发表种属
Human,
Mouse,
Rabbit,
Rat
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
88B
抗原
Purified Torpedo californica acetylcholine receptor.
偶联物
形式
Liquid
浓度
Conc. Not Determined
保存液
ascites
内含物
0.05% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
MA3-043 detects nicotinic acetylcholine receptor (nAChR) gamma and delta subunits in torpedo and the delta subunit in mouse, human, rat, chicken and frog. This antibody does not detect the alpha 1 and beta 1 subunits.
MA3-043 has been successfully used in Western blot, immunohistochemistry and immunoprecipitation procedures. By Western blot, this antibody detects a 60 kDa protein representing the gamma subunit and a 65 kDa protein representing the delta subunit from torpedo skeletal muscle homogenates. Under non-reducing conditions the delta subunit migrates mostly as a dimer of ~130 kDa. Immunohistochemical staining of nAChR in rat skeletal muscle with MA3-043 results in strong staining of the motor endplate.
In Immunohistochemical staining, cryostat or permeabilization of the sections is recommended due to the reactivity of this antibody with the cytoplasmic side of the receptor.
The MA3-043 antigen is purified Torpedo californica acetylcholine receptor.
靶标信息
Acetylcholine receptors (AChRs) mediate synaptic transmission at the neuromuscular junction. The channel-linked AChR that mediates rapid, excitatory actions of acetylcholine is called nicotinic AChR (nAChR) because it can be activated by nicotine. The non-channel linked AChR that medicates the slow actions of acetylcholine, which can be either inhibitory or excitatory, is called muscarinic AChR (mAChR) because it can be activated by muscarine.The nAChRs fall into two subclasses, the muscle-type and the neuronal-type. The muscle-type receptor is a pentameric glycoprotein of five membrane spanning subunits, (2 alpha 1's, beta 1, gamma or epsilon and delta) which form a ligand gated ion channel. The neuronal nAChR is also a pentamer, but unlike the muscle-type, is made up of only two general types of subunits, alpha and beta of which multiple subtypes have been described (alpha 2 to alpha 8 and beta 2 to beta 4).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: acetylcholine receptor; Acetylcholine receptor subunit alpha; acetylcholine receptor, nicotinic, alpha 1 (muscle); alpha-1 subunit, nicotinic acetylcholine receptor; cholinergic receptor; cholinergic receptor nicotinic alpha polypeptide 1; cholinergic receptor, nicotinic alpha 1; cholinergic receptor, nicotinic, alpha 1 (muscle); cholinergic receptor, nicotinic, alpha polypeptide 1 (muscle); muscle nicotinic acetylcholine receptor; nAChR; nicotinic acetylcholine receptor alpha subunit; nicotinic cholinergic receptor alpha 1
基因别名: Achr-1; ACHRA; ACHRD; Acra; AI385656; AI608266; CHNRA; CHRNA; CHRNA1; CMS1A; CMS1B; CMS2A; FCCMS; NACHRA1; SCCMS
UniProt ID: (Chicken) P09479, (Human) P02708, (Mouse) P04756, (Rat) P25108
Entrez Gene ID: (Chicken) 395608, (Human) 1134, (Mouse) 11435, (Rat) 79557
transmembrane signaling receptor activity
acetylcholine-gated cation channel activity
neurotransmitter receptor activity
acetylcholine-activated cation-selective channel activity
ion channel activity
ligand-gated ion channel activity
acetylcholine receptor activity
acetylcholine binding
extracellular ligand-gated ion channel activity
protein binding
ligand-gated ion channel
signal transduction
chemical synaptic transmission
ion transmembrane transport
regulation of membrane potential
neurological system process
excitatory postsynaptic potential
skeletal muscle contraction
transport
synaptic transmission, cholinergic
neuromuscular synaptic transmission
neuromuscular junction development
neuronal action potential
response to nicotine
muscle cell cellular homeostasis
skeletal muscle tissue growth
musculoskeletal movement
neuromuscular process
neuron cellular homeostasis
cation transmembrane transport
ion transport
cation transport
