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Invitrogen
CHST4 Polyclonal Antibody
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CHST4 Antibody (PA5-47752) in WB
Western blot analysis of CHST4 in bEnd.3 mouse endothelioma cell line. Samples were incubated in CHST4 polyclonal antibody (Product # PA5-47752) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Sheep IgG secondary antibody. A specific band was detected for Carbohydrate Sulfotransferase 4/CHST4 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-47752
产品规格
种属反应
Mouse
宿主/亚型
Sheep
/ IgG
分类
Polyclonal
类型
Antibody
抗原
CHO-derived recombinant mouse Carbohydrate Sulfotransferase 4/CHST4 Arg30-Gly388
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
浓度
0.2 mg/mL
规格
100 µg
纯化类型
Antigen affinity chromatography
保存液
PBS with 5% trehalose
内含物
No Preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
In direct ELISAs and Western blots, approximately 40% cross-reactivity with recombinant human (rh) CHST4 is observed and less than 5% cross-reactivity with recombinant mouse CHST5 and rhCHST6 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
靶标信息
This gene encodes an N-acetylglucosamine 6-O sulfotransferase. The encoded enzyme transfers sulfate from 3'phosphoadenosine 5'phospho-sulfate to the 6-hydroxyl group of N-acetylglucosamine on glycoproteins. This protein is localized to the Golgi and is involved in the modification of glycan structures on ligands of the lymphocyte homing receptor L-selectin. Alternate splicing in the 5' UTR results in multiple transcript variants that encode the same protein.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: carbohydrate (chondroitin 6/keratan) sulfotransferase 4; galactose/N-acetylglucosamine/N-acetylglucosamine; galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 3; glcNAc6ST-2; high endothelial cell GlcNAC-6-sulphotransferase; high endothelial cells N-acetylglucosamine 6-O-sulfotransferase; L-selectin ligand sulfotransferase; LSST; N-acetylglucosamine 6-O-sulfotransferase 2; unnamed protein product
基因别名: Gn6st-2; GST-3; HEC-GlcNAc6ST
UniProt ID: (Mouse) Q9R1I1
Entrez Gene ID: (Mouse) 26887
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