Invitrogen

Calsequestrin Monoclonal Antibody (VIIID12)

Name: Calsequestrin Monoclonal Antibody (VIIID12)
Brand: Invitrogen
SKU: MA3-913

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36个已发表图片

79 篇参考文献

查看其他5个Calsequestrin抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

Calsequestrin Antibody in Immunocytochemistry (ICC/IF)

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Calsequestrin Antibody (MA3-913) in ICC/IF

Immunofluorescent analysis of Calsequestrin using Anti-Calsequestrin Monoclonal Antibody (VIIID12) (Product # MA3-913) shows staining in MCF-7 Cells. Calsequestrin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizin... View More

Calsequestrin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Calsequestrin Antibody in Western Blot (WB)

Calsequestrin Antibody in Immunohistochemistry (IHC)

Calsequestrin Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Calsequestrin Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

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Immunogen image

Immunogen Image Calsequestrin Monoclonal Antibody (VIIID12)

产品信息

MA3-913

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看64篇已发表文章 64篇已发表文章

免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:20

免疫组织化学（多聚甲醛固定） (IHC (PFA))

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免疫细胞化学 (ICC/IF)

1:50-1:500

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免疫沉淀 (IP)

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亲和纯化 (AP)

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产品规格

种属反应

Dog, Chicken, Human, Mouse, Pig, Rabbit, Rat

已发表种属

C. elegans, Chicken, Dog, Human, Mouse, Non-human primate, Pig, Rabbit, Rat, Rodent

宿主/亚型

Mouse / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

VIIID12

抗原

Purified rabbit skeletal muscle sarcoplasmic reticulum.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

Conc. Not Determined

保存液

PBS, ascites

内含物

0.05% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_325496

产品详细信息

MA3-913 detects calsequestrin from human, mouse, rat, canine, porcine, chicken, and rabbit skeletal muscle tissues. This antibody recognizes calsequestrin in both type I (slow) and type II (fast) skeletal muscle tissues.

MA3-913 has been successfully used in Western blot procedures. By Western blot, this antibody detects a 63 kDa protein representing calsequestrin from canine skeletal muscle extracts. Higher molecular weight proteins are seen on the Western blot and are believed to be calsequestrin-like proteins found in the sarcoplasmic reticulum. The staining pattern yields double rows of fluorescent dots corresponding to triad pairs on either side of the Z-line.

The MA3-913 antigen is purified rabbit skeletal muscle sarcoplasmic reticulum.

靶标信息

The sarcoplasmic reticulum (SR) is, in part, responsible for maintaining the level of intracellular calcium in cardiac and skeletal muscle by storing and releasing calcium. Several intralumenal SR calcium binding proteins have been identified, the most prominent of these is calsequestrin. Calsequstrin is a calcium binding protein known to sequester calcium accumulated in the sarcoplasmic reticulum of muscle cells during relaxation and is found discretely localized to the junctional and corbular (terminal cisternae) SR. Calsequestrin functions to localize calcium near the junctional face of the terminal cisternae from which calcium can be released into the cytosol via the ryanodine receptor. This protein is highly acidic and has a large capacity and moderate to low affinity for calcium.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: Aspartactin; calmitin; Calmitine; calsequestrin; calsequestrin 1 (fast-twitch, skeletal muscle); Calsequestrin, skeletal muscle isoform; Calsequestrin-1; calsquestrin 1; Laminin-binding protein; skeletal muscle calsequestrin 1

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基因别名: CASQ; CASQ1; CSQ; CSQ-1; CSQ1; PDIB1; sCSQ; VMCQA