Invitrogen

Complement Factor I Polyclonal Antibody

Name: Complement Factor I Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-79036
Availability: InStock

查看其他15个Complement Factor I抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (3)

Complement Factor I Antibody in Western Blot (WB)

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Complement Factor I Antibody (PA5-79036) in WB

Western blot analysis of Complement Factor I in Lane 1: rat liver tissue lysate, Lane 2: HeLa whole cell lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, incubated with Complement Factor I polyclonal antibody (Product # PA5-79036) at a dilution of 0.5 µg/mL (overnight ... View More

Complement Factor I Antibody in Flow Cytometry (Flow)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

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Immunogen image

Immunogen Image Complement Factor I Polyclonal Antibody

产品信息

PA5-79036

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

免疫组化（冰冻） (IHC (F))

0.5-1 µg/mL

免疫细胞化学 (ICC/IF)

0.5-1 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

产品规格

种属反应

Human, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E. coli-derived human Factor I recombinant protein (Position: K19-D220).

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

100 µg

纯化类型

Antigen affinity chromatography

保存液

PBS with 5mg BSA

内含物

0.05mg sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2746152

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: rat liver tissue, HELA whole cell. Flow: U-87 cell, HEPG2 cell.

靶标信息

Factor I, a serine protease, is a regulatory component of both the alternative and the classical complement pathway. Factor I is synthesized as a single chain precursor of 88 kDa, processed to yield the active form, composed of two disulfide-linked chains, the a-chain (50 kDa) and the b-chain (38 kDa). Plasma concentration is 35 µg/mL.

仅用于科研。不用于诊断过程。未经明确授权不得转售。