Invitrogen

DR3 Recombinant Polyclonal Antibody (11HCLC)

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DR3 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence was performed on fixed and permeabilized K562 cells for detection of endogenous DR3/TNFRSF25 using Anti- DR3/TNFRSF25 Recombinant Rabbit Polyclonal Antibody (Product # 711309, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of DR3/TNFRSF25 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating membrane localization of DR3/TNFRSF25. Panel e) represents control cells with no primary antibody to assess background.

DR3 Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extracts (30 µg lysate) of K-562 (Lane 1), Jurkat (Lane 2), KARPAS 299 (Lane 3), THP-1 (Lane 4), PC-3 (Lane 5), SH-SY5Y (Lane 6), HeLa (Lane 7) and Caco-2 (Lane 8). The blots were probed with Anti-DR3/TNFRSF25 Recombinant Rabbit Polyclonal Antibody (Product # 711309, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 45kDa band along with 13 kDa isoform corresponding to DR3/TNFRSF25 was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).

产品信息

711309

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1-2 µg/mL	-
免疫细胞化学 (ICC/IF)	2 µg/mL	-

产品规格
种属反应	Human, Mouse, Rat
宿主/亚型	Rabbit / IgG
分类	Recombinant Polyclonal
类型	Antibody
克隆号	11HCLC
抗原	Peptides corresponding to Human TNFRSF25 (aa 398-414, 29-42)
偶联物	Unconjugated
形式	Liquid
浓度	0.5 mg/mL
纯化类型	Protein A
保存液	PBS, pH 7.2
内含物	0.09% sodium azide
保存条件	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2633156

产品详细信息

This antibody is predicted to react with Monkey, Cat, Pig, Sheep and Bovine.

Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.

靶标信息

DR3/TNFRSF25 is a member of the TNF-receptor superfamily. This receptor is expressed preferentially in the tissues enriched in lymphocytes, and it may play a role in regulating lymphocyte homeostasis. This receptor has been shown to stimulate NF-kappa B activity and regulate cell apoptosis. The signal transduction of this receptor is mediated by various death domain containing adaptor proteins. Knockout studies in mice suggested the role of DR3/TNFRSF25 in the removal of self-reactive T cells in the thymus. Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported, most of which are potentially secreted molecules. The alternative splicing of this gene in B and T cells encounters a programmed change upon T-cell activation, which predominantly produces full-length, membrane bound isoforms, and is thought to be involved in controlling lymphocyte proliferation induced by T-cell activation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Apo-3; apoptosis inducing receptor; Apoptosis-inducing receptor AIR; Apoptosis-mediating receptor DR3; Apoptosis-mediating receptor TRAMP; death domain receptor 3 soluble form; Death receptor 3; death receptor beta; LARD; Lymphocyte-associated receptor of death; Protein WSL; Protein WSL-1; RP4-650H14.2; Tumor necrosis factor receptor superfamily member 25; tumor necrosis factor receptor superfamily, member 12; tumor necrosis factor receptor superfamily, member 12 (translocating chain-association membrane protein); tumor necrosis factor receptor superfamily, member 25

基因别名: APO-3; APO3; DDR3; DR3; LARD; TNFRSF12; TNFRSF25; TR3; TRAMP; UNQ455/PRO779; WSL; WSL-1; WSL-LR; WSL1

UniProt ID: (Human) Q93038

Entrez Gene ID: (Human) 8718, (Rat) 500592, (Mouse) 85030

分子生物学功能: transmembrane signal receptor