Invitrogen

EGFRvIII Recombinant Rabbit Monoclonal Antibody (RM419), Biotin

查看其他2个EGFRvIII抗体

问题与答案

问题与答案

抗体检测数据 (4)

EGFRvIII Antibody in Western Blot (WB)

EGFRvIII Antibody in Western Blot (WB)

图: 1 / 4

EGFRvIII Antibody (MA562099) in WB

Western Blot analysis of DKMG (EGFRvIII positive), U118MG, A431, and HeLa whole cell lysates using EGFRvIII recombinant monoclonal antibody (Product # MA562099) at 1:1,000 dilution.

EGFRvIII Antibody in Western Blot (WB)

EGFRvIII Antibody in Immunohistochemistry (Paraffin) (IHC (P))

EGFRvIII Antibody in Flow Cytometry (Flow)

EGFRvIII Antibody in ELISA (ELISA)

Product Image Gallery

EGFRvIII Antibody in Western Blot (WB) — Western Blot analysis of DKMG (EGFRvIII positive), U118MG, A431, and HeLa whole cell lysates using EGFRvIII recombinant monoclonal antibody (Product # MA562099) at 1:1,000 dilution.

EGFRvIII Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of human Glioblastoma Multiforme tissue sections with or without EGFRvIIII using EGFRvIII recombinant monoclonal antibody (Product # MA562099) at 1:100 dilution.

EGFRvIII Antibody in Flow Cytometry (Flow) — Flow cytometry analysis of DKMG and 293 cells, using EGFRvIII recombinant monoclonal antibody (Product # MA562099) at 1:50 dilution.

EGFRvIII Antibody in ELISA (ELISA) — ELISA analysis of EGFRvIII recombinant monoclonal antibody (Product # MA562099) showing clone RM419 does not react to wild type EGFR protein. The plate was coated with recombinant EGFR variant III and EGFRwt proteins. A serial dilution of anti-EGFRvlll (Clone RM419) or anti-EGFR (Clone RM229) antibodies were used.

产品信息

MA562099

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:2,000

-

免疫组化（石蜡） (IHC (P))

1:100-1:200

-

流式细胞分析 (Flow)

1:10-1:100

-

酶联免疫吸附实验 (ELISA)

1:100-1:1,000

-

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

RM419

抗原

A peptide corresponding to epidermal growth factor receptor variant III (EGFRvIII).

偶联物

Biotin Biotin Biotin

形式

Liquid

浓度

Conc. Not Determined

纯化类型

Protein A

保存液

PBS with 1% BSA, 50% glycerol

内含物

0.09% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

This antibody reacts to human EGFRvIII.

靶标信息

EGFRvIII is included in receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin. Isoform 2 may act as an antagonist of EGF action.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; Epidermal growth factor receptor; erb-b2 receptor tyrosine kinase 1; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

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基因别名: EGFR; ERBB; ERBB1; HER1; mENA; NISBD2; PIG61

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UniProt ID: (Human) P00533

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Entrez Gene ID: (Human) 1956

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chromatin binding double-stranded DNA binding MAP kinase kinase kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity transmembrane signaling receptor activity epidermal growth factor-activated receptor activity Ras guanyl-nucleotide exchange factor activity protein binding ATP binding enzyme binding protein phosphatase binding nitric-oxide synthase regulator activity ubiquitin protein ligase binding identical protein binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity protein heterodimerization activity actin filament binding cadherin binding involved in cell-cell adhesion transmembrane signal receptor

MAPK cascade activation of MAPKK activity cell morphogenesis ossification embryonic placenta development positive regulation of protein phosphorylation hair follicle development response to stress signal transduction cell surface receptor signaling pathway epidermal growth factor receptor signaling pathway activation of phospholipase C activity salivary gland morphogenesis learning or memory cell proliferation positive regulation of cell proliferation regulation of phosphatidylinositol 3-kinase signaling single organismal cell-cell adhesion peptidyl-tyrosine phosphorylation cerebral cortex cell migration positive regulation of cell growth positive regulation of cell migration positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle positive regulation of catenin import into nucleus ERBB2 signaling pathway negative regulation of epidermal growth factor receptor signaling pathway negative regulation of protein catabolic process positive regulation of phosphorylation activation of phospholipase A2 activity by calcium-mediated signaling negative regulation of apoptotic process positive regulation of MAP kinase activity positive regulation of GTPase activity positive regulation of nitric oxide biosynthetic process positive regulation of DNA repair positive regulation of DNA replication positive regulation of transcription from RNA polymerase II promoter protein autophosphorylation phosphatidylinositol phosphorylation phosphatidylinositol-mediated signaling positive regulation of fibroblast proliferation digestive tract morphogenesis positive regulation of epithelial cell proliferation regulation of peptidyl-tyrosine phosphorylation regulation of nitric-oxide synthase activity protein insertion into membrane positive regulation of protein kinase B signaling morphogenesis of an epithelial fold eyelid development in camera-type eye response to UV-A positive regulation of ERK1 and ERK2 cascade cellular response to amino acid stimulus cellular response to epidermal growth factor stimulus cellular response to estradiol stimulus positive regulation of production of miRNAs involved in gene silencing by miRNA regulation of cell motility