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Invitrogen
Fc Receptor Binding Inhibitor Polyclonal Antibody, eBioscience™
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Fc Receptor Binding Inhibitor Antibody (14-9161-73) in Flow
Fc receptor-mediated non-specific binding is blocked by Fc Receptor Binding Inhibitor. THP-1 cells were left untreated (purple histogram) or treated with Fc Receptor Binding Inhibitor Antibody (blue histogram) for 15 minutes at 4C. The cells were then stained with 0.5 µg of Mouse IgG2a kappa Isotype Control, APC (Product # 17-4724-81). The grey histogram represents autofluorescence of unstained cells. Viabl... View More
产品信息
14-9161-73
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
C. elegans,
Fish,
Human,
Mouse,
Pig,
Rhesus monkey,
Yeast,
Zebrafish
分类
Polyclonal
类型
Antibody
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: The human FcgammaR-binding inhibitor can be used to inhibit the non-specific Fc-gamma receptor (FcgammaR)-mediated binding of mouse monoclonal antibodies used for flow cytometric analysis of human tissue. Four different classes of Fcgamma receptors have been identified: FcgammaRI (CD64), FcgammaRII (CD32), and FcgammaRIII (CD16), and they are expressed at varying levels in multiple cell lineages including high expression in myeloid, granulocyte and B cells. The biological function of the FcgammaR, including initiation of endocytosis, phagocytosis and antigen presentation, is elicited upon binding of host-immunoglobulin. The extent to which mouse monoclonal antibodies will bind to human FcgammaR varies depending on the isotype of the monoclonal antibody. Furthermore, different monoclonal antibodies of the same isotype will display different binding to human FcgammaRs.
Applications Reported: Purified Fc Receptor Binding Inhibitor has been reported for use in flow cytometric analysis.
To inhibit the non-specific binding of mouse monoclonal antibodies add the human Fc Receptor Binding Inhibitor to samples and incubate on ice for 20 minutes. Without washing proceed to stain with primary antibody.
Applications Tested: Purified human Fc gammaR-binding inhibitor has been pre-titrated and tested by inhibiting binding of fluorochrome-conjugated isotype controls to U937 cells. This can be used at 20 µL per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Four different classes of Fcy receptors have been identified: FcyRI (CD64), FcyRII (CD32), and FcyRIII (CD16), and they are expressed at varying levels in multiple cell lineages including high expression in myeloid, granulocyte and B cells. The biological function of the FcyR, including initiation of endocytosis, phagocytosis and antigen presentation, is elicited upon binding of host-immunoglobulin. The extent to which mouse monoclonal antibodies will bind to human FcyR varies depending on the isotype of the monoclonal antibody. Furthermore, different monoclonal antibodies of the same isotype will display different binding to human FcyRs.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
