Invitrogen

GABRA5 Polyclonal Antibody

Product Image Gallery

GABRA5 Antibody in Western Blot (WB) — Western Blot analysis of GABRA5 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a GABRA5 Polyclonal Antibody (Product # PA5-31163) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

GABRA5 Antibody in Immunohistochemistry (IHC) — Figure 3. Representative photomicrographs of the CA1 region showing GABA A R alpha1, alpha2, alpha3, alpha5, beta3, and gamma2 subunit expression (red) and alpha1, alpha2, alpha3, alpha5, beta3, and gamma2 immunoreactivity overlaid with Hoechst (blue) labeling for representative young and old mice ( A-X ). The strong alpha2 subunit immunoreactivity is evident around the soma, proximal dendrites, and possibly in the axon-initial segment of individual pyramidal cells of the CA1 region in young animals but the labeling is decreased at these sites in aged mice ( E-H ). Scale bar, 50 mum. Startum (str), oriens (ori), pyramidale (pyr), radiatum (rad).

产品信息

PA5-31163

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1:500-1:3,000	查看2篇已发表文章 2篇已发表文章
免疫组化 (IHC)	-	查看2篇已发表文章 2篇已发表文章

产品规格
种属反应	Human, Mouse
已发表种属	Mouse
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	Recombinant fragment corresponding to a region within amino acids 142 and 379 of Human GABA A Receptor alpha 5
偶联物	Unconjugated
形式	Liquid
浓度	0.93 mg/mL
纯化类型	Antigen affinity chromatography
保存液	PBS, pH 7, with 20% glycerol
内含物	0.025% Proclin 300
保存条件	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2548637

产品详细信息

Recommended positive controls: GABRA5-transfected 293T.

Predicted reactivity: Mouse (100%), Rat (100%), Bovine (98%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

GABRA5 is a part of GABA, a major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. Transcript variants utilizing three different alternative non-coding first exons have been described.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

GABA_A Receptors Are Well Preserved in the Hippocampus of Aged Mice.

eNeuro

Palpagama TH,Sagniez M,Kim S,Waldvogel HJ,Faull RL,Kwakowsky A

PA5-31163 was used in Western Blotting to show, that GABAA Receptors are well preserved in ageing mice.Western blotting and immunohistochemistry were used.

Mon Mar 02 00:00:00 EST 2020

GABA_A Receptors Are Well Preserved in the Hippocampus of Aged Mice.

eNeuro

Palpagama TH,Sagniez M,Kim S,Waldvogel HJ,Faull RL,Kwakowsky A

PA5-31163 was used in Western Blotting to show, that GABAA Receptors are well preserved in ageing mice.Western blotting and immunohistochemistry were used.

Mon Mar 02 00:00:00 EST 2020

Amyloid-Beta_1-42 -Induced Increase in GABAergic Tonic Conductance in Mouse Hippocampal CA1 Pyramidal Cells.

Molecules (Basel, Switzerland)

Calvo-Flores Guzmán B,Kim S,Chawdhary B,Peppercorn K,Tate WP,Waldvogel HJ,Faull RL,Montgomery J,Kwakowsky A

PA5-31163 was used in Immunohistochemistry to show that hippocampal injection of Aβ1-42 is sufficient to induce cognitive deficits 7 days post-injection.

Thu Feb 06 00:00:00 EST 2020

Biology of sex differences

Pandya M,Palpagama TH,Turner C,Waldvogel HJ,Faull RL,Kwakowsky A

Published figure using GABRA5 polyclonal antibody (Product # PA5-31163) in Western Blot

Mon Jan 14 00:00:00 EST 2019

生物信息学

蛋白别名: GABA(A) receptor subunit alpha-5; GABAA receptor alpha5 subunit; gamma-aminobutyric acid; gamma-aminobutyric acid (GABA) A receptor, alpha 5; gamma-aminobutyric acid (GABA-A) receptor, subunit alpha 5; Gamma-aminobutyric acid receptor subunit alpha-5; ligand-gated ion channel subunit

基因别名: A230018I05Rik; GABRA5

UniProt ID: (Human) P31644, (Mouse) Q8BHJ7

Entrez Gene ID: (Human) 2558, (Mouse) 110886

分子生物学功能: ligand-gated ion channel