Invitrogen

GLIS1 Polyclonal Antibody

GLIS1 Antibody in Immunocytochemistry (ICC/IF)

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GLIS1 Antibody (720032) in ICC/IF

Immunofluorescence was performed on fixed and permeabilized SH-SY5Y cells for detection of Glis1 using Anti-Glis1 Rabbit Polyclonal Antibody (Product # 720032, 1 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 0.4 µg/mL, 1:2500). Panel a) shows representative cells that were stained for detection and localization of GLIS1 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938, 1:50). Pa... View More

GLIS1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

720032

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-3 µg/mL

免疫组化（石蜡） (IHC (P))

1:20

免疫细胞化学 (ICC/IF)

1 µg/mL

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Multiple peptides against Human Glis1 (aa 111-128, 308-321, 349-366)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 30% glycerol, 0.1% BSA

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2532790

产品详细信息

This antibody is predicted to react with Monkey, Rat, Mouse, Pig, Bovine, Rabbit and Dog.

靶标信息

Glis1 (Gli similar 1) is also known as Gli related Kruppel-like zinc finger 1 and is encoded by the Glis1 gene located on chromosome 1 in humans. It belongs to the Kruppel-like zinc finger family of proteins that constitute mainly of transcription factors and is hence studied for its role in target gene activation and repression, specifically during embryonic development. Glis1 contains five tandem zinc finger motifs, which is the region at which it shares close homology to Glis2 and Glis 3. More recent studies on Glis1 show its interaction with Oct 3/4, Sox2 and KLF4 in efficient generation of iPSC.

仅用于科研。不用于诊断过程。未经明确授权不得转售。