Invitrogen

GSK3 alpha/beta Monoclonal Antibody (21A)

GSK3 alpha/beta Antibody in Immunocytochemistry (ICC/IF)

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GSK3 alpha/beta Antibody (44-610) in ICC/IF

Immunofluorescence analysis of GSK3 alpha/beta was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with GSK3 alpha/beta Monoclonal Antibody (21A) (Product # 44-610) at 1:100 dilution in 0.1% BSA, incubated at... View More

GSK3 alpha/beta Antibody in Western Blot (WB)

GSK3 alpha/beta Antibody in Western Blot, Immunocytochemistry, Immunoprecipitation (WB, ICC/IF, IP)

GSK3 alpha/beta Antibody in Western Blot, Immunohistochemistry (WB, IHC)

GSK3 alpha/beta Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

产品信息

44-610

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看47篇已发表文章 47篇已发表文章

免疫组化 (IHC)

Assay-Dependent

查看2篇已发表文章 2篇已发表文章

免疫细胞化学 (ICC/IF)

1:100

查看4篇已发表文章 4篇已发表文章

流式细胞分析 (Flow)

Assay-dependent

酶联免疫吸附实验 (ELISA)

1:1,000

免疫沉淀 (IP)

5-10 µg

查看4篇已发表文章 4篇已发表文章

其他PubMed (Misc)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Mouse, Rat, Xenopus, Human

已发表种属

Human, Mouse, Non-human primate, Rat, Shrew

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

21A

抗原

The antibody was produced in mouse against a recombinant Xenopus laevis protein

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS, pH 7.2

内含物

0.01% sodium azide

保存条件

4°C short term, -80°C long term

AB_2533693

产品详细信息

44-610 is an antibody that's produced in mouse against a recombinant Xenopus laevis protein. |Has been successfully used ELISA, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, and Western Blot procedures.

靶标信息

Glycogen synthase kinase-3 (GSK3) is a protein kinase that was originally identified as a regulator of glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. GSK3's substrate specificity is unique in that phosphorylation of the substrate only occurs if a phosphoserine or phosphotyrosine is present - four residues C-terminal to the site of GSK phosphorylation. There exists two isoforms of GSK3, GSK3 alpha and GSK3 beta, and they are strictly regulated via phosphorylation. Phosphorylation of GSK3 beta on Ser9 (Ser21 in GSK3alpha) by protein kinase B (PKB) causes its inactivation and is the primary mechanism responsible for growth factor inhibition of this kinase. Activation of GSK3 beta is dependent upon the phosphorylation of Tyr216 (Tyr279 in GSK3 alpha). Upon activation, it has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor-1 (HSF-1), and cyclin D1. GSK3 beta also has been shown to phosphorylate aberrant sites on the microtubule associated protein tau, which is critical for the progression of Alzheimer's disease.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Mechanical Study of Jian-Gan-Xiao-Zhi Decoction on Nonalcoholic Fatty Liver Disease Based on Integrated Network Pharmacology and Untargeted Metabolomics.

Evidence-based complementary and alternative medicine : eCAM

Cao YJ,Li HZ,Zhao J,Sun YM,Jin XW,Lv SQ,Luo JY,Fang XX,Wen WB,Liao JB

44-610 was used in Western Blotting to investigate the mechanisms by which Jian-Gan-Xiao-Zhi decoction improve nonalcoholic fatty liver disease.

Tue Jul 19 00:00:00 UTC 2022

GSK3α, not GSK3β, drives hippocampal NMDAR-dependent LTD via tau-mediated spine anchoring.

The EMBO journal

Draffin JE,Sánchez-Castillo C,Fernández-Rodrigo A,Sánchez-Sáez X,Ávila J,Wagner FF,Esteban JA

Published figure using GSK3 alpha/beta monoclonal antibody (Product # 44-610) in Western Blot

Fri Jan 15 00:00:00 UTC 2021

A-769662 inhibits adipocyte glucose uptake in an AMPK-independent manner.

The Biochemical journal

Kopietz F,Alshuweishi Y,Bijland S,Alghamdi F,Degerman E,Sakamoto K,Salt IP,Göransson O

44-610 was used in Western Blot to suggest that AMPK activation per se does not inhibit glucose uptake in adipocytes and that the effects of AICAR and A-769662 are AMPK-independent.

Fri Feb 12 00:00:00 UTC 2021

A-769662 inhibits adipocyte glucose uptake in an AMPK-independent manner.

The Biochemical journal

Kopietz F,Alshuweishi Y,Bijland S,Alghamdi F,Degerman E,Sakamoto K,Salt IP,Göransson O

44-610 was used in Western Blot to suggest that AMPK activation per se does not inhibit glucose uptake in adipocytes and that the effects of AICAR and A-769662 are AMPK-independent.

Fri Feb 12 00:00:00 UTC 2021

Glycogen Synthase Kinase 3 Regulates the Genesis of Displaced Retinal Ganglion Cells3.

eNeuro

Kisseleff E,Vigouroux RJ,Hottin C,Lourdel S,Thomas L,Shah P,Chédotal A,Perron M,Swaroop A,Roger JE

Published figure using GSK3 alpha/beta monoclonal antibody (Product # 44-610) in Western Blot

Wed Oct 20 00:00:00 UTC 2021

Glycogen Synthase Kinase 3 Regulates the Genesis of Displaced Retinal Ganglion Cells3.

eNeuro

Kisseleff E,Vigouroux RJ,Hottin C,Lourdel S,Thomas L,Shah P,Chédotal A,Perron M,Swaroop A,Roger JE

Published figure using GSK3 alpha/beta monoclonal antibody (Product # 44-610) in Western Blot

Wed Oct 20 00:00:00 UTC 2021

Glycogen Synthase Kinase 3 Regulates the Genesis of Displaced Retinal Ganglion Cells3.

eNeuro

Kisseleff E,Vigouroux RJ,Hottin C,Lourdel S,Thomas L,Shah P,Chédotal A,Perron M,Swaroop A,Roger JE

Published figure using GSK3 alpha/beta monoclonal antibody (Product # 44-610) in Western Blot

Wed Oct 20 00:00:00 UTC 2021

Inhibition of AMPK activity in response to insulin in adipocytes: involvement of AMPK pS485, PDEs, and cellular energy levels.

American journal of physiology. Endocrinology and metabolism

Kopietz F,Rupar K,Berggreen C,Säll J,Vertommen D,Degerman E,Rider MH,Göransson O

44-610 was used in Western Blot to improve the understanding of how insulin regulates AMP-activated protein kinase activity, and with that, de novo fatty acid synthesis, in adipose tissue.

Tue Sep 01 00:00:00 UTC 2020

Roles of glycogen synthase kinase 3 alpha and calcineurin in regulating the ability of sperm to fertilize eggs.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Dey S,Eisa A,Kline D,Wagner FF,Abeysirigunawardena S,Vijayaraghavan S

44-610 was used in Western Blotting to explore the relationship between calcineurin and GSK3 in sperm with an emphasis on the events of sperm capacitation and fertilization.

Wed Jan 01 00:00:00 UTC 2020

The pivotal role of glycogen synthase kinase 3 (GSK-3) in vomiting evoked by specific emetogens in the least shrew (Cryptotis parva).

Neurochemistry international

Zhong W,Darmani NA

44-610 was used in Western Blotting to support the notion that vomiting is accompanied by a downregulation of GSK-3 activity and pharmacological inhibition of GSK-3 protects against pharmacologically evoked vomiting.

Wed Jan 01 00:00:00 UTC 2020

Estrogens Inhibit Amyloid-β-Mediated Paired Helical Filament-Like Conformation of Tau Through Antioxidant Activity and miRNA 218 Regulation in hTau Mice.

Journal of Alzheimer's disease : JAD

Guglielmotto M,Manassero G,Vasciaveo V,Venezia M,Tabaton M,Tamagno E

44-610 was used in Western Blot to find that biological sex influences the effect of amyloid-β42 (Aβ42) monomers on pathological tau conformational change.

Thu Sep 09 00:00:00 UTC 2021

Age-associated cholesterol reduction triggers brain insulin resistance by facilitating ligand-independent receptor activation and pathway desensitization.

Aging cell

Martín-Segura A,Ahmed T,Casadomé-Perales Á,Palomares-Perez I,Palomer E,Kerstens A,Munck S,Balschun D,Dotti CG

Published figure using GSK3 alpha/beta monoclonal antibody (Product # 44-610) in Western Blot

Sat Jun 01 00:00:00 UTC 2019

Age-associated cholesterol reduction triggers brain insulin resistance by facilitating ligand-independent receptor activation and pathway desensitization.

Aging cell

Martín-Segura A,Ahmed T,Casadomé-Perales Á,Palomares-Perez I,Palomer E,Kerstens A,Munck S,Balschun D,Dotti CG

Published figure using GSK3 alpha/beta monoclonal antibody (Product # 44-610) in Western Blot

Sat Jun 01 00:00:00 UTC 2019

Ovarian Hormone-Dependent Effects of Dietary Lipids on APP/PS1 Mouse Brain.

Frontiers in aging neuroscience

Herrera JL,Ordoñez-Gutierrez L,Fabrias G,Casas J,Morales A,Hernandez G,Acosta NG,Rodriguez C,Prieto-Valiente L,Garcia-Segura LM,Wandosell FG,Alonso R

44-610 was used in Western Blot to suggest that ovarian hormones and dietary composition affect the brain lipidome and amyloid burden in female APP/PS1 mice models.

Wed Sep 30 00:00:00 UTC 2020

Isoform-specific GSK3A activity is negatively correlated with human sperm motility.

Molecular human reproduction

Freitas MJ,Silva JV,Brothag C,Regadas-Correia B,Fardilha M,Vijayaraghavan S

44-610 was used in Western Blotting to demonstrate that sperm motility modulation could be achieved by targeting isoform-specific GSK3A in human sperm.

Mon Apr 01 00:00:00 UTC 2019

GSK3β activity alleviates epileptogenesis and limits GluA1 phosphorylation.

EBioMedicine

Urbanska M,Kazmierska-Grebowska P,Kowalczyk T,Caban B,Nader K,Pijet B,Kalita K,Gozdz A,Devijver H,Lechat B,Jaworski T,Grajkowska W,Sadowski K,Jozwiak S,Kotulska K,Konopacki J,Van Leuven F,van Vliet EA,Aronica E,Jaworski J

Published figure using GSK3 alpha/beta monoclonal antibody (Product # 44-610) in Western Blot

Tue Jan 01 00:00:00 UTC 2019

GSK3β activity alleviates epileptogenesis and limits GluA1 phosphorylation.

EBioMedicine

Published figure using GSK3 alpha/beta monoclonal antibody (Product # 44-610) in Western Blot

Tue Jan 01 00:00:00 UTC 2019

GSK3α and GSK3β Phosphorylate Arc and Regulate its Degradation.

Frontiers in molecular neuroscience

Gozdz A,Nikolaienko O,Urbanska M,Cymerman IA,Sitkiewicz E,Blazejczyk M,Dadlez M,Bramham CR,Jaworski J

44-610 was used in Western Blotting to identify GSK3α/β-catalyzed Arc phosphorylation and degradation as a novel mechanism for controlling the duration of Arc expression and function.

Wed Sep 30 00:00:00 UTC 2020

Lack of liver glycogen causes hepatic insulin resistance and steatosis in mice.

The Journal of biological chemistry

Irimia JM,Meyer CM,Segvich DM,Surendran S,DePaoli-Roach AA,Morral N,Roach PJ

Published figure using GSK3 alpha/beta monoclonal antibody (Product # 44-610) in Western Blot

Fri Jun 23 00:00:00 UTC 2017

Beta-amyloid 1-42 monomers, but not oligomers, produce PHF-like conformation of Tau protein.

Aging cell

Manassero G,Guglielmotto M,Zamfir R,Borghi R,Colombo L,Salmona M,Perry G,Odetti P,Arancio O,Tamagno E,Tabaton M

Published figure using GSK3 alpha/beta monoclonal antibody (Product # 44-610) in Western Blot

Sat Oct 01 00:00:00 UTC 2016

Skeletal Muscle-specific G Protein-coupled Receptor Kinase 2 Ablation Alters Isolated Skeletal Muscle Mechanics and Enhances Clenbuterol-stimulated Hypertrophy.

The Journal of biological chemistry

Woodall BP,Woodall MC,Luongo TS,Grisanti LA,Tilley DG,Elrod JW,Koch WJ

Published figure using GSK3 alpha/beta monoclonal antibody (Product # 44-610) in Western Blot

Fri Oct 14 00:00:00 UTC 2016

Skeletal Muscle-specific G Protein-coupled Receptor Kinase 2 Ablation Alters Isolated Skeletal Muscle Mechanics and Enhances Clenbuterol-stimulated Hypertrophy.

The Journal of biological chemistry

Woodall BP,Woodall MC,Luongo TS,Grisanti LA,Tilley DG,Elrod JW,Koch WJ

Published figure using GSK3 alpha/beta monoclonal antibody (Product # 44-610) in Western Blot

Fri Oct 14 00:00:00 UTC 2016

Class I PI3-kinase or Akt inhibition do not impair axonal polarization, but slow down axonal elongation.

Biochimica et biophysica acta

Diez H,Benitez MJ,Fernandez S,Torres-Aleman I,Garrido JJ,Wandosell F

44610 was used in western blot to propose that PTEN's role in axonal polarity can be independent of PIP3

Tue Nov 01 00:00:00 UTC 2016

Estradiol and Progesterone Administration After pMCAO Stimulates the Neurological Recovery and Reduces the Detrimental Effect of Ischemia Mainly in Hippocampus.

Molecular neurobiology

Perez-Alvarez MJ,Mateos L,Alonso A,Wandosell F

44-610 was used in western blot to investigate the effects of estradiol and progesterone co-administered on ischemia.

Tue Dec 01 00:00:00 UTC 2015

Targeted disruption of glycogen synthase kinase 3A (GSK3A) in mice affects sperm motility resulting in male infertility.

Biology of reproduction

Bhattacharjee R,Goswami S,Dudiki T,Popkie AP,Phiel CJ,Kline D,Vijayaraghavan S

Published figure using GSK3 alpha/beta monoclonal antibody (Product # 44-610) in Western Blot

Sun Mar 01 00:00:00 UTC 2015

Loss of glycogen synthase kinase 3 isoforms during murine oocyte growth induces offspring cardiac dysfunction.

Biology of reproduction

Monteiro da Rocha A,Ding J,Slawny N,Wolf AM,Smith GD

44-610 was used in western blot to study the role of oocyte GSK3 on fertility, fetal development, and offspring health using transgenic mice

Fri May 01 00:00:00 UTC 2015

APPL1 endocytic adaptor as a fine tuner of Dvl2-induced transcription.

FEBS letters

Banach-Orlowska M,Szymanska E,Miaczynska M

44-610 was used in western blot to demonstrate a role for APPL1 in regulating Dvl2-dependent transcriptional activity of AP-1

Fri Feb 13 00:00:00 UTC 2015

Neuritic complexity of hippocampal neurons depends on WIP-mediated mTORC1 and Abl family kinases activities.

Brain and behavior

Franco-Villanueva A,Wandosell F,Antón IM

Published figure using GSK3 alpha/beta monoclonal antibody (Product # 44-610) in Western Blot

Sun Nov 01 00:00:00 UTC 2015

GSK3β inhibition promotes synaptogenesis in Drosophila and mammalian neurons.

PloS one

Cuesto G,Jordán-Álvarez S,Enriquez-Barreto L,Ferrús A,Morales M,Acebes Á

44-610 was used in western blot to determine the the role of GSK3beta in synaptogenesis by comparing rats and fruit flies.

Wed Nov 25 00:00:00 UTC 2015

GSK3β inhibition promotes synaptogenesis in Drosophila and mammalian neurons.

PloS one

Cuesto G,Jordán-Álvarez S,Enriquez-Barreto L,Ferrús A,Morales M,Acebes Á

44-610 was used in western blot to determine the the role of GSK3beta in synaptogenesis by comparing rats and fruit flies.

Wed Nov 25 00:00:00 UTC 2015

GSK3β inhibition promotes synaptogenesis in Drosophila and mammalian neurons.

PloS one

Cuesto G,Jordán-Álvarez S,Enriquez-Barreto L,Ferrús A,Morales M,Acebes Á

44-610 was used in western blot to determine the the role of GSK3beta in synaptogenesis by comparing rats and fruit flies.

Wed Nov 25 00:00:00 UTC 2015

Structural Plasticity of Dendritic Spines Requires GSK3α and GSK3β.

PloS one

Cymerman IA,Gozdz A,Urbanska M,Milek J,Dziembowska M,Jaworski J

Published figure using GSK3 alpha/beta monoclonal antibody (Product # 44-610) in Immunofluorescence

Fri May 13 00:00:00 UTC 2016

Structural Plasticity of Dendritic Spines Requires GSK3α and GSK3β.

PloS one

Cymerman IA,Gozdz A,Urbanska M,Milek J,Dziembowska M,Jaworski J

Published figure using GSK3 alpha/beta monoclonal antibody (Product # 44-610) in Immunofluorescence

Fri May 13 00:00:00 UTC 2016

Glycogen synthase kinase 3β ubiquitination by TRAF6 regulates TLR3-mediated pro-inflammatory cytokine production.

Nature communications

Ko R,Park JH,Ha H,Choi Y,Lee SY

44-610 was used in western blot to study TLR3-mediated pro-inflammatory cytokine production regulated by glycogen synthase kinase 3-beta ubiquitination by TRAF6

Wed Apr 01 00:00:00 UTC 2015

Muscle RING finger-1 attenuates IGF-I-dependent cardiomyocyte hypertrophy by inhibiting JNK signaling.

American journal of physiology. Endocrinology and metabolism

Wadosky KM,Rodríguez JE,Hite RL,Min JN,Walton BL,Willis MS

Published figure using GSK3 alpha/beta monoclonal antibody (Product # 44-610) in Western Blot

Tue Apr 01 00:00:00 UTC 2014

Terminal hypothermic Tau.P301L mice have increased Tau phosphorylation independently of glycogen synthase kinase 3α/β.

The European journal of neuroscience

Maurin H,Lechat B,Borghgraef P,Devijver H,Jaworski T,Van Leuven F

Published figure using GSK3 alpha/beta monoclonal antibody (Product # 44-610) in Western Blot

Tue Jul 01 00:00:00 UTC 2014

Inhibitory phosphorylation of GSK-3β by AKT, PKA, and PI3K contributes to high NaCl-induced activation of the transcription factor NFAT5 (TonEBP/OREBP).

American journal of physiology. Renal physiology

Zhou X,Wang H,Burg MB,Ferraris JD

44-610 was used in western blot to elucidate mechanisms involved in NaCl-induced NFAT5 activity.

Mon Apr 01 00:00:00 UTC 2013

Carboxyl terminus of Hsp70-interacting protein (CHIP) is required to modulate cardiac hypertrophy and attenuate autophagy during exercise.

Cell biochemistry and function

Willis MS,Min JN,Wang S,McDonough H,Lockyer P,Wadosky KM,Patterson C

44-610 was used in western blot to identify the role of carboxyl terminus of Hsp70-interacting protein in cardiac hypertrophy and attenuate autophagy during exercise

Sun Dec 01 00:00:00 UTC 2013

AS160 deficiency causes whole-body insulin resistance via composite effects in multiple tissues.

The Biochemical journal

Wang HY,Ducommun S,Quan C,Xie B,Li M,Wasserman DH,Sakamoto K,Mackintosh C,Chen S

44-610 was used in western blot to study the role of AS160 in the insulin responses in multiple tissues

Tue Jan 15 00:00:00 UTC 2013

Insulin receptor substrate 2 is required for testicular development.

PloS one

Griffeth RJ,Carretero J,Burks DJ

Published figure using GSK3 alpha/beta monoclonal antibody (Product # 44-610) in Western Blot

Tue Feb 18 00:00:00 UTC 2014

Neurological characterization of mice deficient in GSK3α highlight pleiotropic physiological functions in cognition and pathological activity as Tau kinase.

Molecular brain

Maurin H,Lechat B,Dewachter I,Ris L,Louis JV,Borghgraef P,Devijver H,Jaworski T,Van Leuven F

44-610 was used in western blot to investigate the contribution of GSK3α to neurological diseases.

Sat May 25 00:00:00 UTC 2013

Neurological characterization of mice deficient in GSK3α highlight pleiotropic physiological functions in cognition and pathological activity as Tau kinase.

Molecular brain

Maurin H,Lechat B,Dewachter I,Ris L,Louis JV,Borghgraef P,Devijver H,Jaworski T,Van Leuven F

44-610 was used in western blot to investigate the contribution of GSK3α to neurological diseases.

Sat May 25 00:00:00 UTC 2013

Post-ischemic estradiol treatment reduced glial response and triggers distinct cortical and hippocampal signaling in a rat model of cerebral ischemia.

Journal of neuroinflammation

Pérez-Álvarez MJ,Maza Mdel C,Anton M,Ordoñez L,Wandosell F

Published figure using GSK3 alpha/beta monoclonal antibody (Product # 44-610) in Western Blot

Mon Jul 02 00:00:00 UTC 2012

Inhibition of glycogen synthase kinase-3 alleviates Tcf3 repression of the pluripotency network and increases embryonic stem cell resistance to differentiation.

Nature cell biology

Wray J,Kalkan T,Gomez-Lopez S,Eckardt D,Cook A,Kemler R,Smith A

44-610 was used in western blot to demonstrate that β-catenin is not necessary for embryonic stem cell identity or expansion, but its absence eliminates the self-renewal response to Gsk3 inhibition.

Sun Jun 19 00:00:00 UTC 2011

Inhibition of glycogen synthase kinase-3 alleviates Tcf3 repression of the pluripotency network and increases embryonic stem cell resistance to differentiation.

Nature cell biology

Wray J,Kalkan T,Gomez-Lopez S,Eckardt D,Cook A,Kemler R,Smith A

44-610 was used in western blot to demonstrate that β-catenin is not necessary for embryonic stem cell identity or expansion, but its absence eliminates the self-renewal response to Gsk3 inhibition.

Sun Jun 19 00:00:00 UTC 2011

Effect of PI3K- and mTOR-specific inhibitors on spontaneous B-cell follicular lymphomas in PTEN/LKB1-deficient mice.

British journal of cancer

García-Martínez JM,Wullschleger S,Preston G,Guichard S,Fleming S,Alessi DR,Duce SL

44-610 was used in western blot to test if inhibitors of PI3K or mTOR kinase are effective for treating cancer.

Tue Mar 29 00:00:00 UTC 2011

Calpain regulates N-terminal interaction of GSK-3β with 14-3-3ζ, p53 and PKB but not with axin.

Neurochemistry international

Goñi-Oliver P,Avila J,Hernández F

44-610 was used in western blot to analyze the effect of GSK3β truncation on protein-protein interactions.

Mon Aug 01 00:00:00 UTC 2011

Calpain regulates N-terminal interaction of GSK-3β with 14-3-3ζ, p53 and PKB but not with axin.

Neurochemistry international

Goñi-Oliver P,Avila J,Hernández F

44-610 was used in western blot to analyze the effect of GSK3β truncation on protein-protein interactions.

Mon Aug 01 00:00:00 UTC 2011

Role of TAPP1 and TAPP2 adaptor binding to PtdIns(3,4)P2 in regulating insulin sensitivity defined by knock-in analysis.

The Biochemical journal

Wullschleger S,Wasserman DH,Gray A,Sakamoto K,Alessi DR

Tue Mar 01 00:00:00 UTC 2011

Role of TAPP1 and TAPP2 adaptor binding to PtdIns(3,4)P2 in regulating insulin sensitivity defined by knock-in analysis.

The Biochemical journal

Wullschleger S,Wasserman DH,Gray A,Sakamoto K,Alessi DR

Tue Mar 01 00:00:00 UTC 2011

Characterization of PF-4708671, a novel and highly specific inhibitor of p70 ribosomal S6 kinase (S6K1).

The Biochemical journal

Pearce LR,Alton GR,Richter DT,Kath JC,Lingardo L,Chapman J,Hwang C,Alessi DR

Fri Oct 15 00:00:00 UTC 2010

Characterization of PF-4708671, a novel and highly specific inhibitor of p70 ribosomal S6 kinase (S6K1).

The Biochemical journal

Pearce LR,Alton GR,Richter DT,Kath JC,Lingardo L,Chapman J,Hwang C,Alessi DR

Fri Oct 15 00:00:00 UTC 2010

Ku-0063794 is a specific inhibitor of the mammalian target of rapamycin (mTOR).

The Biochemical journal

García-Martínez JM,Moran J,Clarke RG,Gray A,Cosulich SC,Chresta CM,Alessi DR

44-610 was used in western blot to characterize the mTORC1 and 2 inhibitor Ku-0063794

Fri Jun 12 00:00:00 UTC 2009

Insulin promotes glycogen synthesis in the absence of GSK3 phosphorylation in skeletal muscle.

American journal of physiology. Endocrinology and metabolism

Bouskila M,Hirshman MF,Jensen J,Goodyear LJ,Sakamoto K

44-610 was used in western blot to test if dephosphorylation of glycogen synthase mediated via GSK3 is required for normal glycogen synthesis in skeletal muscle

Tue Jan 01 00:00:00 UTC 2008

LY294002 inhibits glucocorticoid-induced COX-2 gene expression in cardiomyocytes through a phosphatidylinositol 3 kinase-independent mechanism.

Toxicology and applied pharmacology

Sun H,Xu B,Sheveleva E,Chen QM

44-610 was used in western blot to test if phosphatidylinositol 3 kinase contributes to corticosterone-induced COX-2

Wed Oct 01 00:00:00 UTC 2008

Phosphorylation of human respiratory syncytial virus P protein at serine 54 regulates viral uncoating.

Virology

Asenjo A,González-Armas JC,Villanueva N

Fri Oct 10 00:00:00 UTC 2008

Measuring GSK3 expression and activity in cells.

Methods in molecular biology (Clifton, N.J.)

Cole AR,Sutherland C

44-610 was used in western blot to discuss the benefits and limitations of different assays to measure glycogen synthase kinase activity

Thu Jan 22 00:00:00 UTC 2009

Kinase activities increase during the development of tauopathy in htau mice.

Journal of neurochemistry

Kelleher I,Garwood C,Hanger DP,Anderton BH,Noble W

Sat Dec 01 00:00:00 UTC 2007

Phosphorylation of beta-catenin by cyclic AMP-dependent protein kinase.

The Journal of biological chemistry

Taurin S,Sandbo N,Qin Y,Browning D,Dulin NO

Published figure using GSK3 alpha/beta monoclonal antibody (Product # 44-610) in Western Blot

Fri Apr 14 00:00:00 UTC 2006

Phosphorylation of beta-catenin by cyclic AMP-dependent protein kinase.

The Journal of biological chemistry

Taurin S,Sandbo N,Qin Y,Browning D,Dulin NO

Published figure using GSK3 alpha/beta monoclonal antibody (Product # 44-610) in Western Blot

Fri Apr 14 00:00:00 UTC 2006

Role that phosphorylation of GSK3 plays in insulin and Wnt signalling defined by knockin analysis.

The EMBO journal

McManus EJ,Sakamoto K,Armit LJ,Ronaldson L,Shpiro N,Marquez R,Alessi DR

Published figure using GSK3 alpha/beta monoclonal antibody (Product # 44-610) in Western Blot

Wed Apr 20 00:00:00 UTC 2005

Role of the PDK1-PKB-GSK3 pathway in regulating glycogen synthase and glucose uptake in the heart.

FEBS letters

Mora A,Sakamoto K,McManus EJ,Alessi DR

Published figure using GSK3 alpha/beta monoclonal antibody (Product # 44-610) in Immunoprecipitation

Mon Jul 04 00:00:00 UTC 2005

Role of the PDK1-PKB-GSK3 pathway in regulating glycogen synthase and glucose uptake in the heart.

FEBS letters

Mora A,Sakamoto K,McManus EJ,Alessi DR

Published figure using GSK3 alpha/beta monoclonal antibody (Product # 44-610) in Immunoprecipitation

Mon Jul 04 00:00:00 UTC 2005

Glycogen synthase kinase-3 interacts with and phosphorylates estrogen receptor alpha and is involved in the regulation of receptor activity.

The Journal of biological chemistry

Medunjanin S,Hermani A,De Servi B,Grisouard J,Rincke G,Mayer D

Published figure using GSK3 alpha/beta monoclonal antibody (Product # 44-610) in Western Blot

Fri Sep 23 00:00:00 UTC 2005

Glycogen synthase kinase-3 interacts with and phosphorylates estrogen receptor alpha and is involved in the regulation of receptor activity.

The Journal of biological chemistry

Medunjanin S,Hermani A,De Servi B,Grisouard J,Rincke G,Mayer D

Published figure using GSK3 alpha/beta monoclonal antibody (Product # 44-610) in Western Blot

Fri Sep 23 00:00:00 UTC 2005

Glycogen synthase kinase-3 interacts with and phosphorylates estrogen receptor alpha and is involved in the regulation of receptor activity.

The Journal of biological chemistry

Medunjanin S,Hermani A,De Servi B,Grisouard J,Rincke G,Mayer D

Published figure using GSK3 alpha/beta monoclonal antibody (Product # 44-610) in Western Blot

Fri Sep 23 00:00:00 UTC 2005

Glycogen synthase kinase-3 interacts with and phosphorylates estrogen receptor alpha and is involved in the regulation of receptor activity.

The Journal of biological chemistry

Medunjanin S,Hermani A,De Servi B,Grisouard J,Rincke G,Mayer D

Published figure using GSK3 alpha/beta monoclonal antibody (Product # 44-610) in Western Blot

Fri Sep 23 00:00:00 UTC 2005

Glycogen synthase kinase-3 interacts with and phosphorylates estrogen receptor alpha and is involved in the regulation of receptor activity.

The Journal of biological chemistry

Medunjanin S,Hermani A,De Servi B,Grisouard J,Rincke G,Mayer D

Published figure using GSK3 alpha/beta monoclonal antibody (Product # 44-610) in Western Blot

Fri Sep 23 00:00:00 UTC 2005

生物信息学

蛋白别名: FA; Factor A; Glycogen synthase kinase-3 alpha; Glycogen synthase kinase-3 beta; GSK-3 alpha; GSK-3 beta; gsk-3-alpha; GSK3beta isoform; Serine/threonine-protein kinase GSK3A; Serine/threonine-protein kinase GSK3B

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基因别名: 2700086H06Rik; 7330414F15Rik; 8430431H08Rik; C86142; GSK-3; GSK-3beta; GSK3; GSK3A; GSK3B

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功能

protein serine/threonine kinase activity protein binding ATP binding protein kinase A catalytic subunit binding tau-protein kinase activity RNA polymerase II transcription factor binding p53 binding protein kinase activity integrin binding beta-catenin binding kinase activity protein kinase binding ubiquitin protein ligase binding ionotropic glutamate receptor binding tau protein binding NF-kappaB binding nucleotide binding transferase activity transferase activity, transferring phosphorus-containing groups transcription factor binding non-receptor serine/threonine protein kinase

参与通路

GSK3 alpha/beta Monoclonal Antibody (21A)

GSK3 alpha/beta Antibody (44-610) in ICC/IF

产品信息

44-610

免疫印迹 (WB)

免疫组化 (IHC)

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

酶联免疫吸附实验 (ELISA)

免疫沉淀 (IP)

其他PubMed (Misc)

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生物信息学

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