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          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • HIF1A Antibodies

          Invitrogen

          HIF1A Monoclonal Antibody (H1alpha67)

          2个已发表图片
          44 篇参考文献
          查看其他65个HIF1A抗体

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          说明书
          实验方案
          问题与答案
          技术支持
          说明书
          实验方案
          问题与答案
          技术支持

          Cite HIF1A Monoclonal Antibody (H1alpha67)

          • 抗体检测数据 (2)
          • 已发表图片 (2)
          HIF1A Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.
          HIF1A Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          图: 1 / 4

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          HIF1A Antibody (MA1-16511) in IHC (P)

          Immunohistochemical analysis of HIF1A in human kidney. Samples were incubated in HIF1A monoclonal antibody (Product # MA1-16511). {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
          已发表图片由___提供 benchsci-logo
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          查看产品

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          HIF1A Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          HIF1A Antibody in Western Blot (WB)
          HIF1A Antibody in Immunohistochemistry (IHC)
          HIF1A Antibody in ChIP Assay (ChIP)

          产品信息

          MA1-16511

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          1:500-1:1,000
          查看12篇已发表文章 12篇已发表文章

          免疫组化 (IHC)

          -
          查看28篇已发表文章 28篇已发表文章

          免疫组化(石蜡) (IHC (P))

          1:100-1:300
          查看1篇已发表文章 1篇已发表文章

          免疫细胞化学 (ICC/IF)

          Assay-Dependent
          查看1篇已发表文章 1篇已发表文章

          流式细胞分析 (Flow)

          1:10-1:1,000
          -

          酶联免疫吸附实验 (ELISA)

          1:100-1:2,000
          -

          免疫沉淀 (IP)

          1:10
          -

          染色质免疫沉淀 实验 (ChIP)

          1:10-1:500
          查看1篇已发表文章 1篇已发表文章

          凝胶阻滞分析 (GS)

          1:1-1:100
          查看1篇已发表文章 1篇已发表文章
          产品规格

          种属反应

          Avian, Bovine, Dog, Ferret, Human, Mouse, Non-human primate, Sheep, Pig, Rabbit, Rat

          已发表种属

          Human, Mouse, Pig, Rabbit, Rat

          宿主/亚型

          Mouse / IgG2b

          分类

          Monoclonal

          类型

          Antibody

          克隆号

          H1alpha67

          抗原

          Fusion protein containing residues 432-528 of human HIF-1alpha
          查看免疫原

          偶联物

          Unconjugated Unconjugated Unconjugated
          • Biotin 
          • DyLight 488
          • DyLight 550
          • DyLight 650
          • Request custom conjugation

          形式

          Liquid

          浓度

          1.0 mg/mL

          纯化类型

          Protein A

          保存液

          PBS with 1% BSA

          内含物

          0.05% sodium azide

          保存条件

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          运输条件

          Ambient (domestic); Wet ice (international)

          RRID

          AB_568568

          产品详细信息

          In Western blot, multiple bands may be seen at 100-120 kDa representing post-translational modification of HIF-1 alpha.

          For WB, testing on nuclear extracts is recommended.

          靶标信息

          HIF1-alpha (HIF1A) is a subunit of HIF1, which is a transcription factor found in mammalian cells cultured under reduced oxygen tension. HIF-1 is a heterodimer consisting of an alpha and beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of transcription factors. HIF1 functions as a transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, HIF-1 activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF1-alpha regulates hypoxia-mediated apoptosis, cell proliferation and tumor angiogenesis. Hypoxia which induces p53 protein accumulation, directly interacts with HIF1-alpha and reduces hypoxia-induced expression of HIF1-alpha by promoting MDM2-mediated ubiquitination and proteasomal degradation under hypoxic conditions. Recent studies suggest that induction of NOX4 by HIF1-alpha contributes to maintain ROS levels after hypoxia and hypoxia-induced proliferation. In humans, it is located on the q arm of chromosome 14. The C-terminal of HIF1A binds to p300. p300/CBP-HIF complexes participate in the induction of hypoxia-responsive genes, including VEGF. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          生物信息学

          蛋白别名: ARNT interacting protein; ARNT-interacting protein; ARNT2; Basic-helix-loop-helix-PAS protein MOP1; bHLHe78; Class E basic helix-loop-helix protein 78; HIF; hif 1; hif 1a; HIF-1 alpha; HIF-1-alpha; HIF1 alpha; HIF1-alpha; hypoxia inducible factor 1 alpha subunit; hypoxia inducible factor 1, alpha subunit; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor 1 alpha; hypoxia-inducible factor 1 alpha isoform I.3; hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); Hypoxia-inducible factor 1-alpha; hypoxia-inducible factor1alpha; Member of PAS protein 1; member of PAS superfamily 1; PAS domain-containing protein 8

          查看更多 收起

          基因别名: AA959795; BHLHE78; HIF-1-alpha; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; HIF1A; HIF1alpha; MOP1; PASD8

          查看更多 收起

          UniProt ID: (Human) Q16665, (Mouse) Q61221, (Rat) O35800

          查看更多 收起

          Entrez Gene ID: (Bovine) 281814, (Pig) 396696, (Sheep) 443519, (Human) 3091, (Dog) 480348, (Rabbit) 100009579, (Mouse) 15251, (Rat) 29560

          查看更多 收起

          功能
          transcription factor activity, sequence-specific DNA binding histone acetyltransferase binding sequence-specific DNA binding protein heterodimerization activity transcription factor activity, transcription factor binding transcription factor activity, RNA polymerase II transcription factor binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding protein binding transcription factor binding enzyme binding protein kinase binding ubiquitin protein ligase binding protein complex binding nuclear hormone receptor binding histone deacetylase binding Hsp90 protein binding RNA polymerase II transcription factor activity, sequence-specific DNA binding DNA binding signal transducer activity protein dimerization activity double-stranded DNA binding basic helix-loop-helix transcription factor
          参与通路
          response to hypoxia positive regulation of vascular endothelial growth factor production axonal transport of mitochondrion positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of transcription from RNA polymerase II promoter in response to hypoxia cellular response to hypoxia negative regulation of transcription from RNA polymerase II promoter angiogenesis neural crest cell migration epithelial to mesenchymal transition embryonic placenta development B-1 B cell homeostasis positive regulation of endothelial cell proliferation heart looping positive regulation of neuroblast proliferation connective tissue replacement involved in inflammatory response wound healing outflow tract morphogenesis cardiac ventricle morphogenesis lactate metabolic process regulation of transcription, DNA-templated transcription from RNA polymerase II promoter cellular iron ion homeostasis acute-phase response signal transduction lactation visual learning response to salt stress response to X-ray regulation of gene expression vascular endothelial growth factor production positive regulation of gene expression positive regulation of epithelial cell migration positive regulation of receptor biosynthetic process response to auditory stimulus response to purine-containing compound response to muscle activity positive regulation of macroautophagy neural fold elevation formation cerebral cortex development negative regulation of bone mineralization positive regulation of vascular endothelial growth factor receptor signaling pathway negative regulation of TOR signaling response to estradiol oxygen homeostasis positive regulation of chemokine production cellular response to insulin stimulus regulation of transforming growth factor beta2 production collagen metabolic process embryonic hemopoiesis cellular response to drug positive regulation of insulin secretion involved in cellular response to glucose stimulus hemoglobin biosynthetic process mRNA transcription from RNA polymerase II promoter positive regulation of apoptotic process response to alkaloid regulation of transcription from RNA polymerase II promoter in response to oxidative stress response to estrogen positive regulation of erythrocyte differentiation positive regulation of angiogenesis positive regulation of cell size positive regulation of glycolytic process negative regulation of vasoconstriction negative regulation of growth muscle cell cellular homeostasis positive regulation of hormone biosynthetic process digestive tract morphogenesis positive regulation of smooth muscle cell proliferation positive regulation of nitric-oxide synthase activity cartilage development response to glucocorticoid elastin metabolic process maternal process involved in female pregnancy intestinal epithelial cell maturation response to fungicide epithelial cell differentiation involved in mammary gland alveolus development iris morphogenesis retina vasculature development in camera-type eye regulation of transcription from RNA polymerase II promoter in response to hypoxia positive regulation of chemokine-mediated signaling pathway negative regulation of thymocyte apoptotic process cellular response to hydrogen peroxide cellular response to lipopolysaccharide cellular response to carbon monoxide cellular response to nitrite cellular response to electrical stimulus cellular response to mechanical stimulus cellular response to cobalt ion cellular response to glucose stimulus cellular response to interleukin-1 cellular response to organic cyclic compound cellular response to light stimulus dopaminergic neuron differentiation response to anesthetic cellular response to toxic substance hypoxia-inducible factor-1alpha signaling pathway regulation of cellular response to hypoxia positive regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway positive regulation of mitophagy regulation of aerobic respiration cellular response to cyanide negative regulation of reactive oxygen species metabolic process negative regulation of mesenchymal cell apoptotic process blood vessel development vasculature development regulation of glycolytic process transcription, DNA-templated regulation of transcription from RNA polymerase II promoter positive regulation of cell proliferation positive regulation of autophagy cell differentiation negative regulation of transcription elongation from RNA polymerase II promoter regulation of cell proliferation glucose homeostasis negative regulation of apoptotic process negative regulation of neuron apoptotic process blood vessel morphogenesis regulation of catalytic activity regulation of thymocyte apoptotic process response to mechanical stimulus response to glucose response to activity response to cobalt ion response to drug cellular response to lipid
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