Invitrogen

HOXB6 Polyclonal Antibody

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HOXB6 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of HOXB6 in paraffin-embedded human lung following steamed antigen retrieval with a citrate buffer, pH 6, using a HOXB6 polyclonal antibody (Product # PA5-37867) at a dilution of 5 µg/mL. Detection was performed using an AP-conjugated secondary antibody.

HOXB6 Antibody in Western Blot (WB) — Western blot of HOXB6 in HEK293 lysate was performed using 35 µg protein in RIPA buffer. Lysates were probed with a HOXB6 polyclonal antibody (Product # PA5-37867) at a dilution of 1 µg/mL and incubated for 1 hour, followed by chemiluminescent detection.

HOXB6 Antibody in Immunocytochemistry (ICC/IF) — Fig. 3 Immunofluorescence of selected cell lines. a Classification profiles (left) and IF expression (middle) of Caov-4 (OV-positive control), HEC-59 (UCEC-positive control), and SK-OV-3 for WT1 (OV biomarker) and HOXB6 (uterine biomarker). The rightmost bar plots quantify the average percentage of positive cells for WT1 (top-right) and HOXB6 (bottom-right). b Classification profiles (left) and IF expression (middle) of Caov-4, NCCIT (germ cell tumor-positive control), and A2780 for WT1 and LIN28A (germ cell tumor biomarker). Classification was performed on replicates of NCCIT RNA-seq profiles. The rightmost bar plots quantify the average percentage of positive cells for WT1 (top-right) and LIN28A (bottom-right). c Classification profiles (left) and IF expression (middle) of Vcap (PRAD positive control), RT4 (BLCA-positive control), and PC-3 for FOLH1 (prostate biomarker) and PPARG (urothelial biomarker). The rightmost bar plots quantify the average percentage of positive cells for FOLH1 (top-right) and PPARG (bottom-right)

产品信息

PA5-37867

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1-3 µg/mL	-
免疫组化（石蜡） (IHC (P))	5-10 µg/mL	-
免疫细胞化学 (ICC/IF)	-	查看1篇已发表文章 1篇已发表文章

产品规格
种属反应	Human
已发表种属	Not Applicable
宿主/亚型	Goat / IgG
分类	Polyclonal
类型	Antibody
抗原	Peptide with sequence EPRKSDCAQDKS, from the internal region of HOXB6
偶联物	Unconjugated
形式	Liquid
浓度	0.5 mg/mL
纯化类型	Antigen affinity chromatography
保存液	TBS, pH 7.3, with 0.5% BSA
内含物	0.02% sodium azide
保存条件	-20°C
RRID	AB_2554475

产品详细信息

This antibody is predicted to react with mouse, rat, dog, pig and cow based on sequence homology.

靶标信息

HOXB6 belongs to the homeobox family. The homeobox genes encode a highly conserved family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, located on different chromosomes, consisting of 9 to 11 genes arranged in tandem. This gene is one of several homeobox HOXB genes located in a cluster on chromosome 17.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Evaluating the transcriptional fidelity of cancer models.

Genome medicine

Peng D,Gleyzer R,Tai WH,Kumar P,Bian Q,Isaacs B,da Rocha EL,Cai S,DiNapoli K,Huang FW,Cahan P

Published figure using HOXB6 polyclonal antibody (Product # PA5-37867) in Immunocytochemistry

Thu Apr 29 00:00:00 EDT 2021

生物信息学

蛋白别名: homeo box 2B; homeo box B6; Homeobox protein Hox-2.2; Homeobox protein Hox-2B; Homeobox protein Hox-B6; Homeobox protein Hu-2

基因别名: Hox-2.2; HOX2; HOX2B; HOXB6; HU-2

UniProt ID: (Human) P17509

Entrez Gene ID: (Human) 3216

分子生物学功能: homeodomain transcription factor

功能

transcription, DNA-templated regulation of transcription, DNA-templated anterior/posterior pattern specification erythrocyte homeostasis embryonic skeletal system morphogenesis

参与通路

transcription factor activity, sequence-specific DNA binding protein binding sequence-specific DNA binding poly(A) RNA binding

HOXB6 Polyclonal Antibody

产品信息

PA5-37867

免疫印迹 (WB)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

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