应用 | 建议稀释比 | 已发表文章 |
---|---|---|
免疫印迹 (WB) |
Assay-dependent | - |
免疫组化 (IHC) |
Assay-dependent | - |
酶联免疫吸附实验 (ELISA) |
Assay-dependent | - |
功能检测 (FN) |
Assay-dependent | - |
对照试剂 (Ctrl) |
Assay-dependent | - |
产品规格 | |
---|---|
种属 |
Human |
表达系统 |
E. coli |
氨基酸序列 |
GKKCIRTPKI SKPIKFELSG CTSMKTYRAK FCGVCTDGRC CTPHRTTTLP VEFKCPDGEV MKKNMMFIKT CACHYNCPGD NDIFESLYYR KMYGDMA |
分子量 |
11.2 kDa |
分类 |
Recombinant |
类型 |
Protein |
纯度 |
≥95% by SDS-PAGE and HPLC |
内毒素浓度 |
<0.1 ng/µg |
活性 |
ED50 = 1 - 2 µg/mL; determined by the dose-dependent proliferation of HUVECs. |
偶联物 |
Unconjugated |
形式 |
Lyophilized |
纯化类型 |
sequential chromatography |
保存液 |
10mM sodium acetate, pH 6 |
内含物 |
no preservative |
保存条件 |
-20°C |
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile, distilled water, to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2°C to 8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein, such as 0.1% BSA and store in working aliquots at -20°C to -80°C.
Storage: The lyophilized protein is stable at room temperature for 1 month, but should be kept at -20°C for long term storage, preferably desiccated. Working aliquots stored with a carrier protein are stable for at least 10 months at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
Connective Tissue Growth Factor (CTGF), also called CCN2, is a member of the CCN family. CTGF is a secreted protein produced by umbilical veins and vascular endothelial cells. CTGF possesses an Insulin-like Growth Factor (IGF)-binding domain, a thrombospondin type 1 domain, and a cysteine knot region. CTGF plays important roles in the proliferation and differentiation of chondrocytes, induces angiogenesis, and promotes cell adhesion in fibroblasts, endothelial, and epithelial cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: 2-Nov; CCN family member 2; Cellular communication network factor 2; Connective tissue growth factor; H-CTGF; Hypertrophic chondrocyte-specific protein 24; IBP-8; IGF-binding protein 8; IGFBP-8; Insulin-like growth factor-binding protein 8; MGC102839
基因别名: CCN2; CTGF; HCS24; IGFBP8; NOV2
UniProt ID: (Human) P29279
Entrez Gene ID: (Human) 1490
分子生物学功能:
growth factor