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Invitrogen
| 应用 | 建议稀释比 | 已发表文章 |
|---|---|---|
对照试剂 (Ctrl) |
Assay-dependent | - |
其他PubMed (Misc) |
- | 查看1篇已发表文章 1篇已发表文章 |
| 产品规格 | |
|---|---|
种属 |
Human |
已发表种属 |
Not Applicable |
表达系统 |
E. coli |
氨基酸序列 |
Human EGF recombinant protein contains 53 amino acids |
分子量 |
6.2 kDa |
分类 |
Recombinant |
类型 |
Protein |
活性 |
1x10^7 Units/mg compared to reference standards |
偶联物 |
Unconjugated |
形式 |
Lyophilized |
浓度 |
0.1 mg/mL |
纯化类型 |
purified |
内含物 |
no preservative |
保存条件 |
-20°C |
This is a recombinant protein suitable for use as a control in ELISA and Western blot applications.
Lyophilized product is stable at room temperature for 3 weeks. Store lyophilized product desiccated at -20°C. Upon reconstitution, store at 4°C for 2-7 days or aliquot and store at -20°C. For long-term storage add either HSA or BSAQ to 0.1%. Avoid repeated freeze-thaw cycles.
Reconstitute using distilled water to a concentration not less than 100 µg/mL.
EGF (Epidermal growth factor) exerts its actions by binding to the EGF Receptor, a 170 kDa protein kinase. Activation of EGFR initiates diverse cellular pathways in response to toxic environmental stimuli, or to EGF binding to the receptor, the EGFR forms homo- or heterodimers with other family members. Each dimeric receptor complex initiates a distinct signaling pathway by recruiting different Src homology 2 (SH2) containing effector proteins. EGF is far and wide expressed in kidney, cerebrum, prostrate and salivary glands. EGF acts as a potent mitogenic factor and the phosphorylated receptor recruits adapter proteins like GRB2 that activates complex downstream signaling cascades. EGF activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STAT modules. Research studies suggest the protein may also play important role in activating the NF-kappa-B signaling cascade. Defects in the EGGF gene are the cause of hypomagnesemia type 4 and dysregulation has been associated with the growth and progression of certain cancers.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: beta-urogastrone; EGF; Epidermal growth factor; H-EGF; Pro-epidermal growth factor; Urogastrone
基因别名: EGF; HOMG4; URG
UniProt ID: (Human) P01133
Entrez Gene ID: (Human) 1950
分子生物学功能:
transmembrane signal receptor
