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Gibco
| 应用 | 建议稀释比 | 已发表文章 |
|---|---|---|
免疫印迹 (WB) |
Assay-dependent | - |
免疫组化 (IHC) |
Assay-dependent | - |
酶联免疫吸附实验 (ELISA) |
Assay-dependent | - |
功能检测 (FN) |
Assay-dependent | - |
对照试剂 (Ctrl) |
Assay-dependent | - |
| 产品规格 | |
|---|---|
种属 |
Human |
表达系统 |
E. coli |
氨基酸序列 |
Human FGF-7 (KGF) recombinant protein contains 163 amino acids |
分子量 |
18.9 kDa |
分类 |
Recombinant |
类型 |
Protein |
纯度 |
≥95% by SDS-PAGE and HPLC |
内毒素浓度 |
<0.1 ng/µg |
活性 |
ED50 = 0.1 - 15 ng/mL; determined by the dose-dependent proliferation of 4MBr-5 cells. |
偶联物 |
Unconjugated |
形式 |
Lyophilized |
纯化类型 |
sequential chromatography |
保存液 |
PBS, pH 7, with 0.25M NaCl |
内含物 |
no preservative |
保存条件 |
-20°C |
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute KGF in 1X PBS to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2°C to 8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein, such as 0.1% BSA and store in working aliquots at -20°C to -80°C.
Storage: The lyophilized protein is stable at room temperature for 1 month, but should be kept at -20°C for long term storage, preferably desiccated. Working aliquots stored with a carrier protein are stable for at least 3 months at -20°C to -80°C. Avoid repeated freeze/thaw cycles.
Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. Growth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: FGF-7; Fibroblast growth factor 7; HBGF-7; Heparin-binding growth factor 7; Keratinocyte growth factor
基因别名: FGF7; HBGF-7; KGF
UniProt ID: (Human) P21781
Entrez Gene ID: (Human) 2252
