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Gibco
| 应用 | 建议稀释比 | 已发表文章 |
|---|---|---|
免疫印迹 (WB) |
Assay-dependent | - |
免疫组化 (IHC) |
Assay-dependent | - |
酶联免疫吸附实验 (ELISA) |
Assay-dependent | - |
功能检测 (FN) |
Assay-dependent | - |
对照试剂 (Ctrl) |
Assay-dependent | - |
| 产品规格 | |
|---|---|
种属 |
Human |
表达系统 |
E. coli |
氨基酸序列 |
Human FGF-acidic (FGF-1) recombinant protein contains 141 amino acids |
分子量 |
15.8 kDa |
分类 |
Recombinant |
类型 |
Protein |
纯度 |
≥95% by SDS-PAGE and HPLC |
内毒素浓度 |
<0.1 ng/µg |
活性 |
ED50 ≤ 0.5 ng/mL; determined by the dose-dependent proliferation of Balb/c 3T3 cells. |
偶联物 |
Unconjugated |
形式 |
Lyophilized |
纯化类型 |
sequential chromatography |
保存液 |
5mM sodium phosphate, pH 8, with 100mM NaCl |
内含物 |
no preservative |
保存条件 |
-20°C |
Carrier-Free
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 5 mM Sodium Phosphate, pH 8.0, to a concentration of less than 0.5 mg/mL. Do not vortex. This solution can be stored at 2°C to 8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein, such as 0.1% BSA and store in working aliquots at -20°C to -80°C.
Storage: The lyophilized protein is stable at room temperature for 1 month, but should be kept at -20°C for long term storage. Working aliquots stored with a carrier protein are stable for at least 10 months at -80°C to -20°C. Avoid repeated freeze/thaw cycles.
FGF1 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: Acidic fibroblast growth factor; aFGF; beta-endothelial cell growth factor; ECGF; Endothelial cell growth factor; endothelial cell growth factor, alpha; endothelial cell growth factor, beta; FGF; FGF-1; Fibroblast growth factor; Fibroblast growth factor 1; fibroblast growth factor 1 (acidic); H-FGF-a; HBGF-1; Heparin-binding growth factor 1; M-FGF-a
基因别名: AFGF; ECGF; ECGF-beta; ECGFA; ECGFB; FGF-1; FGF-alpha; FGF1; FGFA; GLIO703; HBGF-1; HBGF1
UniProt ID: (Human) P05230
Entrez Gene ID: (Human) 2246
