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Gibco
| 应用 | 建议稀释比 | 已发表文章 |
|---|---|---|
免疫印迹 (WB) |
Assay-dependent | - |
免疫组化 (IHC) |
Assay-dependent | - |
酶联免疫吸附实验 (ELISA) |
Assay-dependent | - |
功能检测 (FN) |
Assay-dependent | - |
对照试剂 (Ctrl) |
Assay-dependent | - |
| 产品规格 | |
|---|---|
种属 |
Human |
表达系统 |
Human |
分类 |
Native |
类型 |
Protein |
活性 |
Corning 24-well plates were coated with fibronectin in PBS (0.3 mL/well) at 37°C overnight. After blocking by BSA, TIG-3 cells (5 x 10^4 cells/1 mL DMEM/well) were added and incubated at 37°C for 90 minutes.When cells are coated on fibronectin-coated plates (6 µg/mL), greater than 70% will adhere specifically. |
偶联物 |
Unconjugated |
形式 |
Liquid |
浓度 |
1 mg/mL |
纯化类型 |
Size-exclusion - Gel filtration |
保存液 |
20mM phosphate/0.2µM PBS, pH 7.5 |
内含物 |
no preservative |
保存条件 |
-20°C |
Source: Human plasma
Fibronectin is a disulfide-bonded dimer with a molecular weight range of 230-250 kDa. In the extracellular matrix of several connective tissues and vessels, fibronectin is present as an insoluble protein that is extensively cross-linked by interchain disulfide bonds forming high molecular mass polymers. Fibronectin is most abundant during embryonic development and tissue remodeling. Fibronectin is also present at high concentrations as a soluble plasma protein. Fibronectin is organized as a linear series of repeating modules which form domains for interaction with fibronectin itself, other matrix components (e.g. collagen and heparin) and receptors on cells (e.g. integrins). Fibronectin is present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. Fibronectin has been implicated in carcinoma development in lung cancer. Further, Fibronectin expression is increased especially in non-small cell lung carcinoma. The adhesion of lung carcinoma cells to Fibronectin enhances tumorgenecity and confers resistance to apoptosis induced by standard chemotherapeutic agents. The gene encoding Fibronectin has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some Fibronectin variants has not been determined.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: Anastellin; CIG; Cold insoluble globulin (CIG); Cold-insoluble globulin; DKFZp686F10164; DKFZp686H0342; DKFZp686I1370; DKFZp686O13149; ferritin L subunit; ferritin L-chain; Fibronectin; FN; Migration stimulating factor (MSF); migration-stimulating factor; Ugl-Y1; Ugl-Y2; Ugl-Y3
基因别名: CIG; ED-B; FINC; FN; FN1; FNZ; GFND; GFND2; LETS; MSF
UniProt ID: (Human) P02751
Entrez Gene ID: (Human) 2335
分子生物学功能:
extracellular matrix protein
extracellular matrix structural protein
