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| 应用 | 建议稀释比 | 已发表文章 |
|---|---|---|
免疫印迹 (WB) |
Assay-dependent | 查看2篇已发表文章 2篇已发表文章 |
中和实验 (Neu) |
- | 查看1篇已发表文章 1篇已发表文章 |
对照试剂 (Ctrl) |
Assay-dependent | - |
其他PubMed (Misc) |
- | 查看1篇已发表文章 1篇已发表文章 |
| 产品规格 | |
|---|---|
种属 |
Human |
已发表种属 |
Human |
表达系统 |
Non-human primate |
氨基酸序列 |
Human Furin convertase protein, amino acids 1-713 |
分类 |
Recombinant |
类型 |
Protein |
活性 |
Specific Activity = 1 U/µL; where 1 U is defined as the amount of furin that can release 1 pmol of AMC from the fluorogenic peptide pERTKR-MCA in 1 min. |
偶联物 |
Unconjugated |
形式 |
Liquid |
纯化类型 |
purified |
保存液 |
0.02M Bis-tris with 1mg/mL BSA |
内含物 |
no preservative |
保存条件 |
-80° C, Avoid Freeze/Thaw Cycles |
RP-062 has an activity of 1 U/ul, where 1 U (a unit of activity) is defined as the amount of furin that can release 1 pmol of AMC from the fluorogenic peptide pERTKR-MCA in 1 min.
As a fully functional enzyme, RP-062 can also be used in a variety of enzymatic assays. 1 U (a unit of activity) is defined as the amount of furin that can release 1 pmol of AMC from the fluorogenic peptide pERTKR-MCA in one minute (1 pmol of AMC/min).
This furin construct represents amino acid residues 1-713 from human furin convertase protein.
Furin is a membrane-associated, calcium-dependent, serine protease that belongs to the subtilisin-like prohormone convertase (PC) family. Members of this family of cellular enzymes cleave most prohormones and neuropeptide precursors. Numerous other cellular proteins, some viral proteins, and bacterial toxins that are transported by the constitutive secretory pathway are also targeted for maturation by PCs. Furin and other PC family members share structural similarities which include a heterogeneous ~10 kDa amino-terminal proregion, a highly conserved ~55 kDa subtilisin-like catalytic domain, and carboxyl-terminal domain that is heterogeneous in length and sequence. These enzymes become catalytically active following proregion cleavage within the appropriate cellular compartment. Furin is the only known PC to possess a transmembrane domain. Cleavage of target proteins occurs at the carboxyl-terminus of the furin consensus sequence, RX(K/R)R. It has been shown that the acidic peptide sequence, C771PSDSEEDEG780, localizes furin to the trans-Golgi-network. Phosphorylation of serine residues within this region modulates intracellular routing of furin protein.
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