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Invitrogen
| 应用 | 建议稀释比 | 已发表文章 |
|---|---|---|
免疫印迹 (WB) |
Assay-dependent | - |
酶联免疫吸附实验 (ELISA) |
Assay-dependent | - |
免疫沉淀 (IP) |
Assay-dependent | - |
其他PubMed (Misc) |
- | 查看5篇已发表文章 5篇已发表文章 |
| 产品规格 | |
|---|---|
种属 |
Human |
已发表种属 |
Not Applicable |
表达系统 |
E. coli |
分子量 |
19.2 kDa |
分类 |
Recombinant |
类型 |
Protein |
活性 |
EC50 ~ 1 U/mL; determined by use of the cytopathic effect inhibition assay described in [Budd et al. (1985) Canc. Chem. Pharm. 12:39]. The units are determined by use of a Human IFN Alpha A (Hu IFN- alpha A [2a]) laboratory standard calibrated to the international reference standard for Human Interferon Alpha A provided by the National Institutes of Health [Meager et al. (2001) J. Immunol Methods. 257(1-2):17-33]. |
偶联物 |
Unconjugated |
形式 |
Liquid |
浓度 |
Lot-specific |
纯化类型 |
Ion-exchange chromatography, Size-exclusion chromatography |
保存液 |
PBS with 0.1% BSA |
内含物 |
no preservative |
保存条件 |
-80° C, Avoid Freeze/Thaw Cycles |
Store at -70°C or below for retention of full activity. Thaw product vial by incubation in cold tap water until just thawed. Aliquot and refreeze unused contents to minimize effects of freeze thawing. Refreezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of product should be in buffers containing protein such as 0.1% BSA or tissue culture media with serum. Dilution for freezing of material below 2 x 10^5 units/mL is not recommended. One freeze thaw cycle is equivalent to thawing an aliquot prepared from the material received.
Human Interferon-alpha 2a produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 19241 Dalton. The Interferon-a 2a gene was obtained from human leukocytes. The rHuIFN-a 2a is purified by proprietary chromatographic techniques.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: alpha-2a interferon; IFN-alpha-2; interferon alpha 2a; interferon alpha 2b; interferon alpha A; Interferon alpha-2; Interferon alpha-A; interferon, alpha 2; LeIF A
基因别名: IFN-alphaA; IFNA; IFNA2; IFNA2A; IFNA2B; IFNA2C; INFA2
UniProt ID: (Human) P01563
Entrez Gene ID: (Human) 3440
分子生物学功能:
interferon superfamily
