Invitrogen

Human IFN-alpha A (alpha 2a) Recombinant Protein

产品信息

111001

应用	建议稀释比	已发表文章
免疫印迹 (WB)	Assay-dependent	-
酶联免疫吸附实验 (ELISA)	Assay-dependent	-
免疫沉淀 (IP)	Assay-dependent	-
其他PubMed (Misc)	-	查看5篇已发表文章 5篇已发表文章

产品规格
种属	Human
已发表种属	Not Applicable
表达系统	E. coli
分子量	19.2 kDa
分类	Recombinant
类型	Protein
活性	EC50 ~ 1 U/mL; determined by use of the cytopathic effect inhibition assay described in [Budd et al. (1985) Canc. Chem. Pharm. 12:39]. The units are determined by use of a Human IFN Alpha A (Hu IFN- alpha A [2a]) laboratory standard calibrated to the international reference standard for Human Interferon Alpha A provided by the National Institutes of Health [Meager et al. (2001) J. Immunol Methods. 257(1-2):17-33].
偶联物	Unconjugated
形式	Liquid
浓度	Lot-specific
纯化类型	Ion-exchange chromatography, Size-exclusion chromatography
保存液	PBS with 0.1% BSA
内含物	no preservative
保存条件	-80° C, Avoid Freeze/Thaw Cycles

产品详细信息

Store at -70°C or below for retention of full activity. Thaw product vial by incubation in cold tap water until just thawed. Aliquot and refreeze unused contents to minimize effects of freeze thawing. Refreezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of product should be in buffers containing protein such as 0.1% BSA or tissue culture media with serum. Dilution for freezing of material below 2 x 10^5 units/mL is not recommended. One freeze thaw cycle is equivalent to thawing an aliquot prepared from the material received.

靶标信息

Human Interferon-alpha 2a produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 19241 Dalton. The Interferon-a 2a gene was obtained from human leukocytes. The rHuIFN-a 2a is purified by proprietary chromatographic techniques.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

The Polybasic Cleavage Site in SARS-CoV-2 Spike Modulates Viral Sensitivity to Type I Interferon and IFITM2.

Journal of virology

Winstone H,Lista MJ,Reid AC,Bouton C,Pickering S,Galao RP,Kerridge C,Doores KJ,Swanson CM,Neil SJD

Mon Apr 12 00:00:00 EDT 2021

A Comprehensive Map of the Monocyte-Derived Dendritic Cell Transcriptional Network Engaged upon Innate Sensing of HIV.

Cell reports

Johnson JS,De Veaux N,Rives AW,Lahaye X,Lucas SY,Perot BP,Luka M,Garcia-Paredes V,Amon LM,Watters A,Abdessalem G,Aderem A,Manel N,Littman DR,Bonneau R,Ménager MM

Tue Jan 21 00:00:00 EST 2020

ARID3a gene profiles are strongly associated with human interferon alpha production.

Journal of autoimmunity

Ratliff ML,Garton J,Garman L,Barron MD,Georgescu C,White KA,Chakravarty E,Wren JD,Montgomery CG,James JA,Webb CF

Tue Jan 01 00:00:00 EST 2019

Nitazoxanide Inhibits Human Norovirus Replication and Synergizes with Ribavirin by Activation of Cellular Antiviral Response.

Antimicrobial agents and chemotherapy

Dang W,Xu L,Ma B,Chen S,Yin Y,Chang KO,Peppelenbosch MP,Pan Q

Thu Nov 01 00:00:00 EDT 2018

Reshaping of the Dendritic Cell Chromatin Landscape and Interferon Pathways during HIV Infection.

Cell host & microbe

Johnson JS,Lucas SY,Amon LM,Skelton S,Nazitto R,Carbonetti S,Sather DN,Littman DR,Aderem A

Wed Mar 14 00:00:00 EDT 2018

生物信息学

蛋白别名: alpha-2a interferon; IFN-alpha-2; interferon alpha 2a; interferon alpha 2b; interferon alpha A; Interferon alpha-2; Interferon alpha-A; interferon, alpha 2; LeIF A

基因别名: IFN-alphaA; IFNA; IFNA2; IFNA2A; IFNA2B; IFNA2C; INFA2

UniProt ID: (Human) P01563

Entrez Gene ID: (Human) 3440

分子生物学功能: interferon superfamily

功能

T cell activation involved in immune response natural killer cell activation involved in immune response apoptotic process inflammatory response humoral immune response cell surface receptor signaling pathway cell-cell signaling blood coagulation negative regulation of gene expression cytokine-mediated signaling pathway B cell differentiation positive regulation of peptidyl-serine phosphorylation of STAT protein B cell proliferation positive regulation of phosphorylation positive regulation of tyrosine phosphorylation of Stat3 protein response to exogenous dsRNA innate immune response negative regulation of T cell differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated negative regulation of viral entry into host cell defense response to virus type I interferon signaling pathway regulation of type I interferon-mediated signaling pathway negative regulation of T-helper 2 cell cytokine production negative regulation of interleukin-5 secretion negative regulation of interleukin-13 secretion

参与通路

cytokine activity type I interferon receptor binding protein binding

Human IFN-alpha A (alpha 2a) Recombinant Protein

产品信息

111001

免疫印迹 (WB)

酶联免疫吸附实验 (ELISA)

免疫沉淀 (IP)

其他PubMed (Misc)

种属

已发表种属

表达系统

分子量

分类

类型

活性

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Brands

Shopping Tools

eSolutions

Support

我们的公司

Your items have has been added!