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| 应用 | 建议稀释比 | 已发表文章 |
|---|---|---|
免疫印迹 (WB) |
Assay-dependent | - |
免疫组化 (IHC) |
Assay-dependent | - |
酶联免疫吸附实验 (ELISA) |
Assay-dependent | - |
功能检测 (FN) |
Assay-dependent | - |
对照试剂 (Ctrl) |
Assay-dependent | - |
| 产品规格 | |
|---|---|
种属 |
Human |
表达系统 |
HEK293 cells |
标签 |
His-tag |
分子量 |
26 kDa |
分类 |
Recombinant |
类型 |
Protein |
纯度 |
&(8)805;95% by SDS-PAGE |
内毒素浓度 |
<1 EU/µg |
活性 |
ED50 ≤ 0.2 µg/mL; determined by the dose-dependent inhibition of MCF-7 cell proliferation in the presence of IGF2 (14 ng/mL). |
偶联物 |
Unconjugated |
形式 |
Lyophilized |
纯化类型 |
sequential chromatography |
内含物 |
no preservative |
保存条件 |
4° C |
Carrier-Free
Reconstitution: We recommend that the vial be briefly centrifuged to bring the contents to the bottom prior to opening. Lyophilized recombinant human IGFBP-4 should be reconstituted in sterile PBS to 0.1-1.0 mg/mL to regain full activity. These stock solutions should be apportioned into working aliquots and stored at -20°C. Further dilutions should be made in medium or buffered solution containing carrier protein, such as PBS with 0.1% BSA.
Storage: Lyophilized human IGFBP-4 should be stored at 2-8°C, preferably desiccated. Store reconstituted human IGFBP-4 at -20°C (not in a frost-free freezer). Keep freeze-thaw cycles to a minimum.
This gene is a member of the insulin-like growth factor binding protein family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors I and II and circulates in the plasma in both glycosylated and non-glycosylated forms. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: IBP-4; IGF-binding protein 4; IGFBP; Insulin like growth factor binding protein; insulin-like growth factor binding protein 4; Insulin-like growth factor-binding protein 4
基因别名: BP-4; HT29-IGFBP; IBP4; IGFBP-4; IGFBP4
UniProt ID: (Human) P22692
Entrez Gene ID: (Human) 3487
分子生物学功能:
protease inhibitor
