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Invitrogen
| 应用 | 建议稀释比 | 已发表文章 |
|---|---|---|
功能检测 (FN) |
Assay-Dependent | - |
对照试剂 (Ctrl) |
Assay-Dependent | - |
| 产品规格 | |
|---|---|
种属 |
Human |
表达系统 |
E. coli |
氨基酸序列 |
Human IL-3, amino acids Ala20-Phe152 (Accession # NM_000558) |
分子量 |
15 kDa |
分类 |
Recombinant |
类型 |
Protein |
纯度 |
>98% by SDS-PAGE and HPLC |
内毒素浓度 |
<0.1 ng/µg |
活性 |
ED50 ≤ 0.1 ng/mL; determined by the dose-dependent proliferation of human TF-1 cells. This corresponsdes with a specific activity greater than or equal to 1x10e7 Units/mg. |
偶联物 |
Unconjugated |
形式 |
Lyophilized |
纯化类型 |
Affinity chromatography |
保存液 |
PBS |
内含物 |
no preservative |
保存条件 |
-20°C |
Description: Interleukin 3 (IL-3), also called Mast Cell Growth Factor (MCGF), Multi-Colony Stimulating Factor (multi-CSF), or Eosinophil-CSF (E-CSF), is produced by activated T cells, mast cells and eosinophils. IL-3 is a hematopoietic growth factor that stimulates colony formation of erythroid, megakaryocyte, neutrophil, eosinophil, basophil, mast cell and monocytic lineages. Most of these functions are enhanced or dependent on co-stimulation with other cytokines. Human and mouse IL-3 share only 29% sequence identity at the amino acid level. The bioactivity of IL-3 is highly species specific. Human IL-3 has no activity on mouse cells.
Applications Reported: Human IL-3 Recombinant Protein Carrier-Free is biologically active.
Applications Tested: The bioactivity of this protein was determined by its ability to induce proliferation of human TF-1 cells. The ED50 for this is less than or equal to 0.1 ng/mL, which corresponds to a specific activity of greater than or equal to 1x10^7 Units/mg.
Source: E. coli-expressed amino acids Ala20-Phe152 (Accession #NM_000588).
Bioactivity: The bioactivity of this protein was determined by its ability to induce proliferation of human TF-1 cells. The ED50 for this is less than or equal to 0.1 ng/mL, which corresponds to a specific activity of greater than or equal to 1x10e7 Units/mg.
Endotoxin: Less than 0.1 ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE and HPLC.
Molecular Weight: 15 kDa.
Storage and handling: Use in a sterile environment. Store lyophilized protein at less than or equal to -20°C. Reconstituted protein solution can be stored with carrier protein (e.g., 0.1% BSA) at less than or equal to -20°C.
Interleukin 3 (IL-3), also called Mast Cell Growth Factor (MCGF), Multi-Colony Stimulating Factor (multi-CSF), or Eosinophil-CSF (E-CSF), is produced by activated T cells, mast cells and eosinophils. IL-3 is a hematopoietic growth factor that stimulates colony formation of erythroid, megakaryocyte, neutrophil, eosinophil, basophil, mast cell and monocytic lineages. Most of these functions are enhanced or dependent on co-stimulation with other cytokines. Human and mouse IL-3 share only 29% sequence identity at the amino acid level. The bioactivity of IL-3 is highly species specific. For example, human IL-3 has no activity on mouse cells. IL-3 is a potent growth promoting cytokine. IL-3 is capable of supporting the proliferation of a broad range of hematopoietic cell types, and is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. IL-3 has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: BP/BPA; Colony stimulating factor multiple; colony-stimulating factor, multiple; Eo-CSF; Hematopoietic growth factor; IL-3; ILN; Interleukin; Interleukin 3 (colony stimulating factor; Interleukin-3; Interleukin3; Mast cell growth factor; mast-cell growth factor; MCGF; MGC79398; MGC79399; Multi CSF; Multilineage colony stimulating factor; multilineage-colony-stimulating factor; multiple); Multipotential colony-stimulating factor; P cell stimulating factor; P-cell stimulating factor; P-cell-stimulating factor; PCSA
基因别名: IL-3; IL3; MCGF; MULTI-CSF
UniProt ID: (Human) P08700
Entrez Gene ID: (Human) 3562
