应用 | 建议稀释比 | 已发表文章 |
---|---|---|
免疫印迹 (WB) |
Assay-dependent | - |
酶联免疫吸附实验 (ELISA) |
Assay-dependent | - |
免疫沉淀 (IP) |
Assay-dependent | - |
对照试剂 (Ctrl) |
Assay-dependent | - |
产品规格 | |
---|---|
种属 |
Human |
表达系统 |
E. coli |
氨基酸序列 |
Human IL-7 recombinant protein contains 153 amino acids |
分子量 |
17.4 kDa |
分类 |
Recombinant |
类型 |
Protein |
纯度 |
>98% by SDS-PAGE and HPLC |
活性 |
ED50 ≤ 0.5 ng/mL; determined by the dose-dependent proliferation of mouse 2E8 cells. |
偶联物 |
Unconjugated |
形式 |
Lyophilized |
纯化类型 |
purified |
内含物 |
no preservative |
保存条件 |
-20° C, Avoid Freeze/Thaw Cycles |
Use: Human IL-7 exerts activity in the range of 0.1 to 10 ng/mL for most in vitro applications.
Reconstitution and Dilution: Reconstitute in distilled water to a concentration of 100 µg/mL. After reconstitution, dilute cytokine in a neutral buffer containing carrier protein such as 5-10% FBS or 1-4% high purity BSA (fatty acid free fraction V).
Lyophilized product is stable until expiration if stored desiccated below 0°C. Cytokines diluted as indicated above should be divided into aliquots and stored at -70°C. Recombinant proteins should be stored at concentrations >500 ng/mL to reduce loss of activity.
Interleukin 7 (IL-7) is a hematopoietic cytokine that is constitutively produced by stromal cells from the bone marrow and thymus. IL-7 signals through the IL-7R to promote the differentiation of hematopoietic stem cells to lymphoid precursor cells, which give rise to T cells, B cells, and natural killer cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: H-IL-7; IL-7; ILN; Interleukin; Interleukin-7; Interleukin7; M-IL-7
基因别名: IL-7; IL7
UniProt ID: (Human) P13232
Entrez Gene ID: (Human) 3574