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| 应用 | 建议稀释比 | 已发表文章 |
|---|---|---|
对照试剂 (Ctrl) |
Assay-dependent | - |
其他PubMed (Misc) |
- | 查看1篇已发表文章 1篇已发表文章 |
| 产品规格 | |
|---|---|
种属 |
Human |
已发表种属 |
Not Applicable |
表达系统 |
Insect |
标签 |
His-tag |
分子量 |
0.05252 kDa |
分类 |
Recombinant |
类型 |
Protein |
活性 |
Specific Activity > 200 mU/mg; with 1 U defined as the activity that hydrolyzes 1 µmol peptide within 1 minute in an enzyme assay. |
偶联物 |
Unconjugated |
形式 |
Liquid |
浓度 |
0.2 mg/mL |
纯化类型 |
purified |
保存液 |
TBS, pH 7.5, with 0.05% Brij-35, 5mM CaCl2 |
内含物 |
no preservative |
保存条件 |
-80° C, Avoid Freeze/Thaw Cycles |
RP-77530 is a purified recombinant MMP-13 protein, expressed in Sf9-insect cells using the baculovirus expression vector system. Upon incubation with APMA, active collagenase-3 is formed. RP-77530 may be used as a control with corresponding anti-MMP-13 antibodies.
MMP13 (collagense 3) plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC, and ACAN. MMP13 cleaves tripal helical collagens, including type I, II, and III collagen - but has the highest activity with soluble type II collagen. It can degrade collagen type IV, XIV, and X. MMP13 plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization, and ossification. It is required for normal embryonic bone development and ossificaiton. Mutations affecting the gene can lead to spondyloepimetaphyseal dysplasia Missouri type, metaphyseal anadysplasia 1, and metaphyseal dysplasia Sphar type.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: Collagenase; Collagenase 3; Collagenase3; matrix metallo protease; matrix metalloproteinase 13 (collagenase 3); Matrix metalloproteinase-13; MMP; MMP-13; MMPs; Procollagenase 3
基因别名: CLG3; MANDP1; MMP-13; MMP13
UniProt ID: (Human) P45452
Entrez Gene ID: (Human) 4322
分子生物学功能:
metalloprotease
